Skewed immunological balance between Th17 (CD4+IL17A+) and Treg (CD4+CD25+FOXP3+) cells in human oral squamous cell carcinoma

Gaur, Poonam; Qadir, Gulam Abdul; Upadhyay, Shilpy; Singh, Avadhesh Kumar; Shukla, Nootan Kumar; Das, Srinibas

doi:10.1007/s13402-012-0093-5

Cited by 40 publications

(49 citation statements)

References 34 publications

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“…Taken together with previous studies investigating Treg cells in these tumors, 16,17 these findings corroborate the presence of this T cell subpopulation in the microenvironment of LLSCC. In the present study, 14 On the other hand, Gaur et al 12 observed a larger number of Treg cells in the peripheral blood of patients with oral SCC who had regional lymph node metastases and were in advanced clinical stages.…”

Section: 15mentioning

confidence: 40%

“…10 Several studies have associated a larger number of Treg cells in the tumor microenvironment with a reduced antitumor immune response and consequent poor prognosis. 6,11 In squamous cell carcinoma (SCC) of the oral cavity, a larger number of Treg cells have been associated with the presence of regional lymph node metastasis, 12 advanced clinical stage, 12 and low overall survival 13 and disease-free survival rates.…”

Section: 8mentioning

confidence: 99%

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Immunohistochemical analysis of FoxP3+ regulatory T cells in lower lip squamous cell carcinomas

et al. 2016

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The aim of this study was to determine the number of FoxP3+ regulatory T (Treg) cells in the microenvironment of lower lip squamous cell carcinomas (LLSCCs) and to correlate the findings with clinicopathological parameters (tumor size/extent, regional lymph node metastasis, clinical stage, and histopathological grade of malignancy). Fifty cases of LLSCC were selected. Lymphocytes exhibiting nuclear immunostaining for FoxP3 were quantified in 10 microscopic fields at the deep invasive front of LLSCCs. The results were analyzed statistically using the nonparametric Mann-Whitney test and Fisher's exact test. FoxP3+ lymphocytes were observed in all cases studied. The number of these cells tended to be higher in smaller tumors, tumors without regional lymph node metastasis, and tumors in early clinical stages, but the difference was not statistically significant (p > 0.05). Low-grade tumors contained a larger number of

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Section: 15mentioning

confidence: 40%

Section: 8mentioning

confidence: 99%

Immunohistochemical analysis of FoxP3+ regulatory T cells in lower lip squamous cell carcinomas

et al. 2016

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“…Furthermore, even established Th17 cells can be induced to produce IFN-γ in addition to IL17A (Th17/Th1 cells), suggesting a common developmental association between Th17 and at least a subset of Th1 cells (28). Notably, numerous studies have reported increased expression of IL17/Th17 cells in different cancers, including colorectal (29), prostate (30), gastric (31), lung (32), ovarian (33), oral (34) and head and neck (35) carcinomas.…”

Section: Discussionmentioning

confidence: 99%

Role of T helper 17 cytokines in the tumour immune inflammation response of patients with laryngeal squamous cell carcinoma

Tang

Tao

Lu³

et al. 2017

Oncology Letters

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Altered cytokine production can lead to immune dysfunction in patients with cancer. The present study investigated the expression of T helper (Th)17 cytokines in patients with laryngeal squamous cell carcinoma (LSCC) and their clinical significance in providing new therapeutic insights. The prevalence of Th17 cells and their receptors in patients with LSCC was studied using immunohistochemical analysis via tissue microarray technology. Flow cytometry was used to investigate the percentage of Th17 and Th1 cells in peripheral blood mononuclear cells. Furthermore, the proliferation of Th17 cells and Th17-associated cytokines, including interleukin (IL)17, IL23 and RAR-related orphan receptor γt, was analyzed by reverse transcription-quantitative polymerase chain reaction. The results revealed that the prevalence of Th17 cells in patients with LSCC was elevated in their primary tumors, as well as in peripheral blood, compared with that in healthy controls. It was further demonstrated that Th17 cells could be induced and expanded in the tumor microenvironment through cytokines produced by the tumor cells. In conclusion, Th17 cells have a substantial impact on the carcinogenesis of LSCCs, and could serve as a potential therapeutic target to modulate the anti-tumor response in these carcinomas.

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“…Induction of immune surveillance is often observed in the early stage of OSCC [20]. However, the local and systemic cell-mediated response is suppressed following disease progression in cancer patients leading to the escape of tumor cells [21]. So far, the exact role of Tregs in the progression of OSCC remains unclear.…”

Section: Discussionmentioning

confidence: 99%

Increased frequency of CD4+ CD25+ FOXP3+ cells correlates with the progression of 4-nitroquinoline1-oxide-induced rat tongue carcinogenesis

et al. 2013

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ObjectivesCD4+ CD25+ FoxP3+ T cells (Tregs) play an essential role in sustaining self-tolerance by negatively regulating immune responses. Increased frequencies of Tregs have been reported in a variety of human cancers. The aim of this study was to evaluate the prevalence of Tregs infiltration in the peripheral blood and regional lymph nodes during rat tongue carcinogenesis induced by 4-nitroquinoline-1-oxide (4NQO).Materials and methodsForty-eight Sprague–Dawley rats were divided into the control (n = 16) and experimental groups (n = 32) to which 4NQO in drinking water was administered. Flow cytometry was used to analyze the prevalence of Tregs in lymphocytes of peripheral blood and regional lymph nodes during 4NQO-induced rat tongue carcinogenesis. CD4+ CD25+ FoxP3+ cells were expressed as a percentage of the total CD4+ cells.ResultsThe frequency of Tregs in peripheral blood from squamous cell carcinoma rats was significantly higher than controls (3.82 ± 0.62 versus 1.40 ± 0.31 %, P < 0.001). The proportion of Tregs was sequentially increased from moderate dysplasia to severe dysplasia and SCC (1.94 ± 0.72, 2.29 ± 0.82, and 3.82 ± 0.62 %, respectively). The frequency of Tregs in regional lymph nodes from squamous cell carcinoma rats was also significantly higher than normal rat mucosa (14.67 ± 3.09 versus 5.53 ± 2.07 %, P < 0.001). The percentage of Tregs was gradually increased in moderate dysplasia, severe dysplasia, and SCC groups (8.93 ± 1.74, 10.15 ± 0.86, 14.67 ± 3.09 %, respectively) as compared to control group (5.53 ± 2.07 %).Conclusion and clinical relevanceTregs in peripheral blood and lymph nodes were associated with disease progression during 4NQO-induced rat tongue carcinogenesis. This study indicated that the upregulation of Tregs might play important role during oral mucosa malignant transformation.

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Skewed immunological balance between Th17 (CD4+IL17A+) and Treg (CD4+CD25+FOXP3+) cells in human oral squamous cell carcinoma

Abstract: Our results suggest an increase in Th17/Tregs ratio in early stages and a decrease in this ratio in higher stages of oral cancer. Such counter regulation of Th17 and Tregs may be a significant prognostic factor in oral cancer patients.

Cited by 40 publications

References 34 publications

Immunohistochemical analysis of FoxP3+ regulatory T cells in lower lip squamous cell carcinomas

Immunohistochemical analysis of FoxP3+ regulatory T cells in lower lip squamous cell carcinomas

Role of T helper 17 cytokines in the tumour immune inflammation response of patients with laryngeal squamous cell carcinoma

Increased frequency of CD4+ CD25+ FOXP3+ cells correlates with the progression of 4-nitroquinoline1-oxide-induced rat tongue carcinogenesis

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