Skeletal tissue engineering using silk biomaterials

MacIntosh, Ana C.; Kearns, Victoria; Crawford, Aileen; Hatton, Paul V.

doi:10.1002/term.68

Cited by 111 publications

(72 citation statements)

References 108 publications

(132 reference statements)

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“…Materials such as collagen, alginate, hyaluronic acid, silk fibroin, chitosan and starch are among the most studied polymers with numerous advantages depending on the specific applications [5][6][7][8][9][10][11][12][13]. One of the most relevant benefits of using materials of natural origin is their biodegradability inside the human body.…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of starch-poly-ε-caprolactone microparticles incorporating bioactive agents for drug delivery and tissue engineering applications

et al. 2009

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One limitation associated with the delivery of bioactive agents concerns the short half-life of these molecules when administered intravenously, which results in their loss from the desired site. Incorporation of bioactive agents into depot vehicles provides a means to increase their persistence at the disease site. Major issues are involved in the development of a proper carrier system able to deliver the correct drug, at the desired dose, place and time. In this work, starch-poly-e-caprolactone (SPCL) microparticles were developed for use in drug delivery and tissue engineering (TE) applications. SPCL microparticles were prepared by using an emulsion solvent extraction/evaporation technique, which was demonstrated to be a successful procedure to obtain particles with a spherical shape (particle size between 5 and 900 lm) and exhibiting different surface morphologies. Their chemical structure was confirmed by Fourier transform infrared spectroscopy. To evaluate the potential of the developed microparticles as a drug delivery system, dexamethasone (DEX) was used as model drug. DEX, a well-known component of osteogenic differentiation media, was entrapped into SPCL microparticles at different percentages up to 93%. The encapsulation efficiency was found to be dependent on the polymer concentration and drug-to-polymer ratio. The initial DEX release seems to be governed mainly by diffusion, and it is expected that the remaining DEX will be released when the polymeric matrix starts to degrade. In this work it was demonstrated that SPCL microparticles containing DEX can be successfully prepared and that these microparticular systems seem to be quite promising for controlled release applications, namely as carriers of important differentiation agents in TE.

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Section: Introductionmentioning

confidence: 99%

Preparation and characterization of starch-poly-ε-caprolactone microparticles incorporating bioactive agents for drug delivery and tissue engineering applications

et al. 2009

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“…I n addition to being used as sutures, silk fibers hold great potential as biomaterials for wound dressings, artificial ligaments, tendons, tissue scaffolds, microcapsules, and other applications (1)(2)(3). Silkworms are the current biological source of silk sutures, but spider silk fibers have superior mechanical properties that are ideal for procedures requiring finer sutures, such as ocular, neurological, and cosmetic surgeries (2,4,5).…”

mentioning

confidence: 99%

“…Silkworms can be cultivated en masse, but territorialism and cannibalism preclude spider farming as a viable manufacturing approach (2). Thus, there is great interest in developing an inexpensive, convenient, and reliable biotechnological approach that can be used to manufacture spider silk fibers as biomaterials (3,6).…”

mentioning

confidence: 99%

Silkworms transformed with chimeric silkworm/spider silk genes spin composite silk fibers with improved mechanical properties

Teulé

Miao

Sohn

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

239

158

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The development of a spider silk-manufacturing process is of great interest. However, there are serious problems with natural manufacturing through spider farming, and standard recombinant protein production platforms have provided limited progress due to their inability to assemble spider silk proteins into fibers. Thus, we used piggyBac vectors to create transgenic silkworms encoding chimeric silkworm/spider silk proteins. The silk fibers produced by these animals were composite materials that included chimeric silkworm/spider silk proteins integrated in an extremely stable manner. Furthermore, these composite fibers were, on average, tougher than the parental silkworm silk fibers and as tough as native dragline spider silk fibers. These results demonstrate that silkworms can be engineered to manufacture composite silk fibers containing stably integrated spider silk protein sequences, which significantly improve the overall mechanical properties of the parental silkworm silk fibers.biomaterials | biotechnology | transgenic animals

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“…The steam sterilization process can be useful for silk fibroin films: several authors have applied moist heat sterilization whithout any appreciable fibroin alteration (16,17,(39)(40)(41). On the other side, Lawrence et al (25) observed that the sterilization with autoclave increased Silk II structure because hydrostatic pressure increases elastic modulus and then crystalline structure content; saturated vapor environment decreases the Tg of silk fibroin, that combined with high temperature increases fibroin chain movement.…”

Section: Discussionmentioning

confidence: 99%

Formulation and Characterization of Silk Fibroin Films as a Scaffold for Adipose-Derived Stem Cells in Skin Tissue Engineering

Chlapanidas

Tosca

Faragò

et al. 2013

Int J Immunopathol Pharmacol

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Skin substitutes are epidermal, dermal or complete bilayered constructs, composed by natural or synthetic scaffolds and by adherent cells such as fibroblasts, keratinocytes or mesenchymal stem cells. Silk fibroin is a promising polymer to realize scaffolds, since it is biocompatible, biodegradable, and exhibits excellent mechanical properties in terms of tensile strength. Moreover, fibroin can be added of others components in order to modify the biomaterial properties for the purpose. The aim of this work is to prepare silk fibroin films for adipose-derived stem cell (ADSCs) culture as a novel feeder layer for skin tissue engineering. Pectin has been added to promote the protein conformational transition and construct strength, while glycerol as plasticizer, providing biomaterial flexibility. Eighteen formulations were prepared by casting method using fibroin, pectin (range 1-10% w/w), and glycerol (range 0-20% w/w); films were characterized by Fourier transform infrared spectroscopy and differential scanning calorimetry assay, to select the optimal composition. A stable fibroin conformation was obtained using 6% w/w pectin, and the best mechanical properties were obtained using 12% w/w glycerol. Films were sterilized, and human ADSCs were seeded and cultured for 15 days. Cells adhere to the support assuming a fibroblastic-like shape and reaching confluence. The ultrastructural analysis evidences typical active-cell features and adhesion structures that promote cell anchorage to the film, thus developing a multilayered cell structure. This construct could be advantageously employed in cutaneous wound healing or where the use of ADSCs scaffold is indicated either in human or veterinary field.The development of skin substitutes dates back to 1975, when Rheinwald and Green (1) cultured keratinocytes on a layer of lethally irradiated 3T3 murine fibroblasts. Currently, this method remains the most reliable and widely used for in vitro keratinocyte culture, but some concerns are emerging about their safety (2-5). To overcome these limits, Sugiyama et al. (6) proposed the use of human irradiated adipose-derived stem cells (ADSCs) as a feeder cell layer: results showed that the morphology of irradiated stem cells was similar to 3T3, and both cell lines (stem and 3T3 cells) expressed genes promoting keratinocyte proliferation. Other researchers also showed that non-irradiated ADSCs can boost epithelialization, angiogenesis (7), capillary density and granulation thickness of transplanted cell-enriched collagen sponges (8). Altman et al. (9) observed that ADSCs, once seeded on acellular dermal matrix and applied on murine wound, spontaneously differentiated along vascular endothelial, fibroblastic and epidermal epithelial lineages and significantly improved wound healing; moreover, cells localize at implantation site.Current cutaneous substitutes are epidermal, dermal or

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Skeletal tissue engineering using silk biomaterials

Cited by 111 publications

References 108 publications

Preparation and characterization of starch-poly-ε-caprolactone microparticles incorporating bioactive agents for drug delivery and tissue engineering applications

Preparation and characterization of starch-poly-ε-caprolactone microparticles incorporating bioactive agents for drug delivery and tissue engineering applications

Silkworms transformed with chimeric silkworm/spider silk genes spin composite silk fibers with improved mechanical properties

Formulation and Characterization of Silk Fibroin Films as a Scaffold for Adipose-Derived Stem Cells in Skin Tissue Engineering

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