Skeletal stem cell physiology on functionally distinct titania nanotopographies

McNamara, Laura E.; Sjöström, Terje; Burgess, Karl; Kim, Joseph J.W.; Liu, Er; Gordonov, Simon; Moghe, Prabhas V.; Meek, R.M. Dominic; Oreffo, Richard O.C.; Su, Bo; Dalby, Matthew J.

doi:10.1016/j.biomaterials.2011.06.063

Cited by 117 publications

(135 citation statements)

References 37 publications

Supporting

Mentioning

121

Contrasting

Unclassified

Order By: Relevance

“…With the rapid development of nanotechnology, surface engineering of titanium using nanotopography to regulate cell behavior is now attracting much research attention. 8,9 In our previous research, we confirmed that hierarchically microstructured or nanostructured surfaces 10 and nanostructured titanium substrates 11 could efficiently regulate the proliferation and differentiation of MSC.…”

Section: Introductionsupporting

confidence: 54%

Effects of titanium nanoparticles on adhesion, migration, proliferation, and differentiation of mesenchymal stem cells

Hou¹,

Cai²,

Li³

et al. 2013

IJN

View full text Add to dashboard Cite

Background:The purpose of this study was to investigate the influences of nanoscale wear particles derived from titanium/titanium alloy-based implants on integration of bone. Here we report the potential impact of titanium oxide (TiO 2 ) nanoparticles on adhesion, migration, proliferation, and differentiation of mesenchymal stem cells (MSC) from the cellular level to the molecular level in the Wistar rat. Methods: A series of TiO 2 nanoparticles (14 nm, 108 nm, and 196 nm) were synthesized and characterized by scanning electron microscopy and transmission electron microscopy, respectively. Results: The TiO 2 nanoparticles had negative effects on cell viability, proliferation, and the cell cycle of MSC in a dose-dependent and size-dependent manner. Confocal laser scanning microscopy was used to investigate the effects of particle internalization on adhesion, spreading, and morphology of MSC. The integrity of the cell membrane, cytoskeleton, and vinculin of MSC were negatively influenced by large TiO 2 nanoparticles. Conclusion: The Transwell migration assay and a wound healing model suggested that TiO 2 nanoparticles had a strong adverse impact on cell migration as particle size increased (P , 0.01). Furthermore, alkaline phosphatase, gene expression of osteocalcin (OC) and osteopontin (OPN), and mineralization measurements indicate that the size of the TiO 2 nanoparticles negatively affected osteogenic differentiation of MSC.

show abstract

Section: Introductionsupporting

confidence: 54%

Effects of titanium nanoparticles on adhesion, migration, proliferation, and differentiation of mesenchymal stem cells

Hou¹,

Cai²,

Li³

et al. 2013

IJN

View full text Add to dashboard Cite

show abstract

“…21 An increased capacity for protein synthesis on the titanium substrates was proposed as explanation for the accumulation of amino acids. Concerning our materials, cells cultured on PEG-based gels (PEG, 3BC and 8-PEG) possibly increased their capacity for protein synthesis as well.…”

Section: Papermentioning

confidence: 99%

Cell phenotypic changes of mouse connective tissue fibroblasts (L-929) to poly(ethylene glycol)-based gels

et al. 2013

View full text Add to dashboard Cite

a Cellular responses to various gels fabricated by photoinitiated crosslinking using acrylated linear and multi-arm poly(ethylene glycol) (PEG)-based and poly(propylene glycol)-b-poly(ethylene glycol) precursors were investigated. While no protein adsorption and cell adhesion were observed on the hydrophilic PEG-based gels, protein adsorption and cell adhesion did occur on the more hydrophobic gel generated from the block copolymer precursor. Murine fibroblast viability on the poly(ethylene glycol)-based gels was studied in the course of 72 h and the results indicated no cytotoxicity. In a systematic study, extraand intracellular metabolites of the murine fibroblasts cultured on these PEG-based gels were examined by GC-MS. Distinct intra-and extracellular changes in primary metabolism, namely amino acid metabolism, glycolysis and fatty acid metabolism, were observed. Cells cultured on the polymeric gels induced more intense intracellular changes in the metabolite profile by means of higher metabolite intensities with time in comparison to cells cultured on the reference substrate (tissue culture polystyrene). In contrast, extracellular changes of metabolite intensities were comparable.

show abstract

“…Hence, adsorption from protein mixtures is a selective phenomenon that leads to enrichment of the interface in particular proteins [11,13,19]. In addition to the time-dependent compositional changes, those proteins that finally remain on the surface may undergo conformational and orientational rearrangements to increase the contact area with the substrate and minimize the interaction energy with it; moreover, as their concentration on the surface increases, they may go through ordering transitions because of the protein-protein interaction [19,[53][54]. Adsorption rates can be increased as a result of increasing surface coverages [55][56][57].…”

Section: Protein Adsorptionmentioning

confidence: 99%

“…The initial cell adhesion of human skeletal mesenchymal stem cells (hMSCs) was evaluated after 3 days of culture on the FN-coated surfaces to ensure a good adhesion on the surface and still be individual cells [54], which allow focusing on the cell-material interaction rather than cell-cell interaction. It is important to note that the initial cell-material interaction occurred in serum-free medium, thus the initial cell contact is only via the initial layer of adsorbed FN on the material surfaces.…”

Section: Cell Adhesionmentioning

confidence: 99%

“…Taking into account the range of stiffness measured on these materials, differentiation along the osteoblastic lineage was determined. The immunofluorescence staining of secreted matrix-associated proteins (OCN and OPN) were used as markers of osteoblastic differentiation [6][7]54]. A methodology to quantify imunofluorescence images for osteogenic markers was developed (see details in the experimental section, page 145) and is shown in In contrast, the dependence of the phosphorylation of Runx2 was upregulated for cells on PBA (x=4), although no significant differences were found between PMA (x=1) and PEA (x=2) (Figure 5.10).…”

Section: Skeletal Stem Cell Differentiationmentioning

confidence: 99%

See 1 more Smart Citation

Biological Activity of Fibronectin at the Cell-Material Interface

García¹

View full text Add to dashboard Cite

Skeletal stem cell physiology on functionally distinct titania nanotopographies

Cited by 117 publications

References 37 publications

Effects of titanium nanoparticles on adhesion, migration, proliferation, and differentiation of mesenchymal stem cells

Effects of titanium nanoparticles on adhesion, migration, proliferation, and differentiation of mesenchymal stem cells

Cell phenotypic changes of mouse connective tissue fibroblasts (L-929) to poly(ethylene glycol)-based gels

Biological Activity of Fibronectin at the Cell-Material Interface

Contact Info

Product

Resources

About