Skeletal myotube formation enhanced by electrospun polyurethane carbon nanotube scaffolds

Sirivisoot, Sirinrath; Harrison, Benjamin S.

doi:10.2147/ijn.s24073

Cited by 66 publications

(61 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, these scaffolds can be loaded with nanoparticles such as carbon nanotubes (CNT) and graphene oxide to increase electrical conductivity of the scaffolds. These particles have been shown to promote myoblast differentiation and also provide a platform which is electrically conductive [56][57][58]. Hence, upon myoblast cell seeding and adhesion, the scaffolds can be electrically stimulated [59].…”

Section: Cell Attachment and Viabilitymentioning

confidence: 99%

Nanofibrous composite scaffolds of poly(ester amides) with tunable physicochemical and degradation properties

Mukundan

Sant

Goenka

et al. 2015

European Polymer Journal

View full text Add to dashboard Cite

iii Polymeric elastomers like Poly (1,3-diamino-2-hydroxypropane-co-polyol sebacate) (APS) have gained importance in soft tissue engineering applications due to their tunable mechanical properties and biodegradability. The fabrication of extracellular matrix (ECM)-mimetic nanofibrous scaffolds using APS is however limited due to its poor solubility in commonly used solvents, low viscosity and high temperatures required for thermal curing. In this study, we have NANOFIBROUS COMPOSITE SCAFFOLDS OF POLY(ESTER AMIDES) WITH TUNABLE PHYSICOCHEMICAL AND DEGRADATION PROPERTIESFNU Shilpaa Mukundan, B.Tech.University of Pittsburgh, 2015 iv scaffolds. Biocompatibility studies using C2C12 mouse myoblast cells showed enhanced cell spreading on APS containing scaffolds after 6h as compared to PCL-only scaffolds. Thus, the present study demonstrates successful development of APS-based thermoset elastomeric nanofibrous scaffolds by blending with semicrystalline PCL polymer for the first time. Tunable physicochemical, mechanical and degradation properties of these composite APS-PCL scaffolds will be further exploited for skeletal muscle tissue engineering applications.

show abstract

Section: Cell Attachment and Viabilitymentioning

confidence: 99%

Nanofibrous composite scaffolds of poly(ester amides) with tunable physicochemical and degradation properties

Mukundan

Sant

Goenka

et al. 2015

European Polymer Journal

View full text Add to dashboard Cite

show abstract

“…Sirivisoot and Harrison (2011) reported that skeletal myotubes cultures grown on a polyurethane-CNTs composite scaffold and electrically stimulated through the scaffold itself, show a higher number and length of cells compared to cultures grown without electrical stimulation. Furthermore, in the presence of electrical stimulation, myotube formation increased in the polyurethane-CNTs composite compared to polyurethane alone.…”

Section: Cnts For Cardiac Cells Electrical Interfacingmentioning

confidence: 99%

Improving cardiac myocytes performance by carbon nanotubes platforms†

Martinelli¹,

Cellot²,

Fabbro³

et al. 2013

Front. Physiol.

View full text Add to dashboard Cite

The application of nanotechnology to the cardiovascular system has increasingly caught scientists' attention as a potentially powerful tool for the development of new generation devices able to interface, repair, or boost the performance of cardiac tissue. Carbon nanotubes (CNTs) are considered as promising materials for nanomedicine applications in general and have been recently tested toward excitable cell growth. CNTs are cylindrically shaped structures made up of rolled-up graphene sheets, with unique electrical, thermal, and mechanical properties, able to effectively conducting electrical current in electrochemical interfaces. CNTs-based scaffolds have been recently found to support the in vitro growth of cardiac cells: in particular, their ability to improve cardiomyocytes proliferation, maturation, and electrical behavior are making CNTs extremely attractive for the development and exploitation of interfaces able to impact on cardiac cells physiology and function.

show abstract

“…For example, myoblasts increase myotube formation on electrically conductive scaffolds, even in the absence of electrical stimuli [14,15]. Extracellular cues influence myotube formation and muscle contraction on electrically conductive scaffolds in the presence of electrical stimulation [16][17][18]. Therefore, by incorporating conducting polymer nanofibres into the three-dimensional scaffolds, the electrical conduction of scaffolds may increase, while maintaining biocompatibility and supporting cell differentiation.…”

Section: Introductionmentioning

confidence: 99%

Protocol and cell responses in three-dimensional conductive collagen gel scaffolds with conductive polymer nanofibres for tissue regeneration

2014

Self Cite

View full text Add to dashboard Cite

It has been established that nerves and skeletal muscles respond and communicate via electrical signals. In regenerative medicine, there is current emphasis on using conductive nanomaterials to enhance electrical conduction through tissue-engineered scaffolds to increase cell differentiation and tissue regeneration. We investigated the role of chemically synthesized polyaniline (PANI) and poly(3,4-ethylenedioxythiophene) (PEDOT) conductive polymer nanofibres for conductive gels. To mimic a naturally derived extracellular matrix for cell growth, type I collagen gels were reconstituted with conductive polymer nanofibres and cells. Cell viability and proliferation of PC-12 cells and human skeletal muscle cells on these three-dimensional conductive collagen gels were evaluated in vitro. PANI and PEDOT nanofibres were found to be cytocompatible with both cell types and the best results (i.e. cell growth and gel electrical conductivity) were obtained with a low concentration (0.5 wt%) of PANI. After 7 days of culture in the conductive gels, the densities of both cell types were similar and comparable to collagen positive controls. Moreover, PC-12 cells were found to differentiate in the conductive hydrogels without the addition of nerve growth factor or electrical stimulation better than collagen control. Importantly, electrical conductivity of the three-dimensional gel scaffolds increased by more than 400% compared with control. The increased conductivity and injectability of the cell-laden collagen gels to injury sites in order to create an electrically conductive extracellular matrix makes these biomaterials very conducive for the regeneration of tissues.

show abstract

Skeletal myotube formation enhanced by electrospun polyurethane carbon nanotube scaffolds

Cited by 66 publications

References 40 publications

Nanofibrous composite scaffolds of poly(ester amides) with tunable physicochemical and degradation properties

Nanofibrous composite scaffolds of poly(ester amides) with tunable physicochemical and degradation properties

Improving cardiac myocytes performance by carbon nanotubes platforms†

Protocol and cell responses in three-dimensional conductive collagen gel scaffolds with conductive polymer nanofibres for tissue regeneration

Contact Info

Product

Resources

About