Skeletal Muscle Triglyceride

Kelley, David E.; Goodpaster, Bret H.

doi:10.2337/diacare.24.5.933

Cited by 402 publications

(270 citation statements)

References 108 publications

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“…Since the present study included an endurancetrained group, this is consistent with data from Goodpaster and colleagues [31], and is in line with previous studies that did not find coupling when muscle triacylglycerol was measured biochemically in samples of muscle tissue [3,32]. It is generally thought that excess intramyocellular lipid storage results in decreased insulin sensitivity because an increased fatty acid precursor supply leads to increased concentrations of one or more of the lipid intermediates: diacylglycerol, long-chain fatty acyl-CoA or ceramide [9,33,34]. In support of this notion, Itani and colleagues found that insulin sensitivity was decreased after increasing plasma NEFA with a lipid infusion during an euglycaemic-hyperinsulinaemic clamp, and this occurred in parallel with an increase in intramuscular diacylglycerol, but not ceramide [15].…”

Section: Basalsupporting

confidence: 92%

Human skeletal muscle ceramide content is not a major factor in muscle insulin sensitivity

et al. 2008

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Aims/hypothesis In skeletal muscle, ceramides may be involved in the pathogenesis of insulin resistance through an attenuation of insulin signalling. This study investigated total skeletal muscle ceramide fatty acid content in participants exhibiting a wide range of insulin sensitivities. Methods The middle-aged male participants (n = 33) were matched for lean body mass and divided into four groups: type 2 diabetes (T2D, n = 8), impaired glucose tolerance (IGT, n = 9), healthy controls (CON, n = 8) and endurancetrained (TR, n = 8). A two step (28 and 80 mU m −2 min −1 ) sequential euglycaemic-hyperinsulinaemic clamp was performed for 120 and 90 min for step 1 and step 2, respectively. Muscle biopsies were obtained from vastus lateralis at baseline, and after steps 1 and 2. Results Glucose infusion rates increased in response to insulin infusion, and significant differences were present between groups (T2D

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Section: Basalsupporting

confidence: 92%

Human skeletal muscle ceramide content is not a major factor in muscle insulin sensitivity

et al. 2008

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“…The evaluation of different fat compartments in the human body is extremely important since regional fat depots and health risks are correlated (125)(126)(127). Many different methods such as MRI/MRSI, computed tomography (CT) and dual-energy X-ray absorptiometry (DXA) are used to evaluate body composition and in particular the regional distribution of fat (125)(126)(127)(128)(129).…”

Section: Mri Of Muscular Fat and Estimation Of Other Lipid Compartmenmentioning

confidence: 99%

“…Many different methods such as MRI/MRSI, computed tomography (CT) and dual-energy X-ray absorptiometry (DXA) are used to evaluate body composition and in particular the regional distribution of fat (125)(126)(127)(128)(129). While many studies have been published that were instrumental in improving understanding of lipid metabolism in general, a review of these publications would be beyond the limits of this article, which focuses on the observation of muscular lipids by means of 1 H-MRS.…”

Section: Mri Of Muscular Fat and Estimation Of Other Lipid Compartmenmentioning

confidence: 99%

Role of proton MR for the study of muscle lipid metabolism

et al. 2006

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1 H-MR spectroscopy (MRS) of intramyocellular lipids (IMCL) became particularly important when it was recognized that IMCL levels are related to insulin sensitivity. While this relation is rather complex and depends on the training status of the subjects, various other influences such as exercise and diet also influence IMCL concentrations. This may open insight into many metabolic interactions; however, it also requires careful planning of studies in order to control all these confounding influences. This review summarizes various historical, methodological, and practical aspects of 1 H-MR spectroscopy (MRS) of muscular lipids. That includes a differentiation of bulk magnetic susceptibility effects and residual dipolar coupling that can both be observed in MRS of skeletal muscle, yet affecting different metabolites in a specific way. Fitting of the intra-(IMCL) and extramyocellular (EMCL) signals with complex line shapes and the transformation into absolute concentrations is discussed. Since the determination of IMCL in muscle groups with oblique fiber orientation or in obese subjects is still difficult, potential improvement with high-resolution spectroscopic imaging or at higher field strength is considered. Fat selective imaging is presented as a possible alternative to MRS and the potential of multinuclear MRS is discussed. 1 H-MRS of muscle lipids allows non-invasive and repeated studies of muscle metabolism that lead to highly relevant findings in clinics and patho-physiology.

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“…Reduced plasma fatty acid uptake and oxidation by the leg [7] and the arm [8,9] have been observed in type 2 diabetics as compared to healthy individuals. Moreover, the relationship between intramuscular lipid accumulation and insulin resistance has been described [10,11]. Physical inactivity or muscle disuse is recognised as one of the risk factors for the development of insulin resistance and type 2 diabetes [12,13].…”

Section: Introductionmentioning

confidence: 99%

Heterogeneity in limb fatty acid kinetics in type 2 diabetes

et al. 2005

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Aims/hypothesis: In order to test the hypothesis that disturbances in skeletal muscle fatty acid metabolism with type 2 diabetes are not equally present in the upper and lower limbs, we studied fatty acid kinetics simultaneously across the arm and leg of type 2 diabetic patients (n=6) and matched control subjects (n=7) for 5 h under baseline conditions and during a 4-h hyperinsulinaemiceuglycaemic clamp. Methods: Limb fatty acid kinetics was determined by means of continuous [U-13 C]palmitate infusion and measurement of arteriovenous differences. Results: The systemic palmitate rate of appearance was 3.6±0.4 and 2.7±0.3 μmol·kg lean body mass −1 ·min −1 and decreased during the clamp by 26% (p=0.04) and 43% (p<0.01) in the diabetic patients and in the control subjects respectively. At baseline, palmitate uptake across the arm was similar in the two groups, whereas leg palmitate uptake was lower than in the arm in the diabetic patients. During the clamp, palmitate uptake decreased in the arm (−48%, p=0.02) and the leg (−38%, p=0.04) of the control subjects, whereas it decreased in the arm (−30%, p=0.04) but not in the leg of the diabetic patients. Similarly, during the clamp palmitate release was substantially suppressed in the arm (−47%, p<0.01) and the leg of the control subjects (−45%, p<0.01), but only in the arm of the diabetic patients (−45%, p<0.01). Conclusions/interpretation: The present data indicate that type 2 diabetes is characterised by heterogeneity in the dysregulation of skeletal muscle fatty acid metabolism, with only the leg, but not the arm, showing an impairment of fatty acid kinetics at baseline and during a hyperinsulinaemic-euglycaemic clamp causing a physiological increase in insulin concentration.

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Skeletal Muscle Triglyceride

Cited by 402 publications

References 108 publications

Human skeletal muscle ceramide content is not a major factor in muscle insulin sensitivity

Human skeletal muscle ceramide content is not a major factor in muscle insulin sensitivity

Role of proton MR for the study of muscle lipid metabolism

Heterogeneity in limb fatty acid kinetics in type 2 diabetes

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