Skeletal muscle tissue engineering

Bach, Alexander D.; Beier, Justus P.; Stern-Staeter, J.; Horch, Raymund E.

doi:10.1111/j.1582-4934.2004.tb00466.x

Cited by 263 publications

(211 citation statements)

References 76 publications

(101 reference statements)

Supporting

Mentioning

208

Contrasting

Unclassified

Order By: Relevance

“…The C2C12 cells were chosen as model cells as this cell line is already widely used for studies of muscle biology and regeneration [26] . Myogenic differentiation on control tissue culture polystyrene surfaces is a well understood process in terms of the sequence of events and protein expression [27] [28] .…”

Section: Introductionmentioning

confidence: 99%

Polyelectrolyte Multilayer Films of Controlled Stiffness Modulate Myoblast Cell Differentiation

Ren

Crouzier

Roy

et al. 2008

Adv Funct Materials

244

263

View full text Add to dashboard Cite

Beside chemical properties and topographical features, mechanical properties of gels have been recently demonstrated to play an important role in various cellular processes, including cell attachment, proliferation, and differentiation. In this work, we used multilayer films made of poly (L-lysine)/Hyaluronan (PLL/HA) of controlled stiffness to investigate the effects of mechanical properties of thin films on skeletal muscle cells (C2C12 cells) differentiation. Prior to differentiation, cells need to adhere and proliferate in growth medium. Stiff films (E 0 > 320 kPa) promoted formation of focal adhesions and organization of the cytoskeleton as well as an enhanced proliferation, whereas soft films were not favorable for cell anchoring, spreading or proliferation. Then C2C12 cells were switched to a low serum containing medium to induce cell differentiation, which was also greatly dependent on film stiffness. Although myogenin and troponin T expressions were only moderately affected by film stiffness, the morphology of the myotubes exhibited striking stiffness-dependent differences. Soft films allowed differentiation only for few days and the myotubes were very short and thick. Cell clumping followed by aggregates detachment could be observed after ~2 to 4 days. On stiffer films, significantly more elongated and thinner myotubes were observed for up to ~ 2 weeks. Myotube striation was also observed but only for the stiffer films. These results demonstrate that film stiffness modulates deeply adhesion, proliferation and differentiation, each of these processes having its own stiffness requirement.

show abstract

Section: Introductionmentioning

confidence: 99%

Polyelectrolyte Multilayer Films of Controlled Stiffness Modulate Myoblast Cell Differentiation

Ren

Crouzier

Roy

et al. 2008

Adv Funct Materials

244

263

View full text Add to dashboard Cite

show abstract

“…Necessary host cell invasion and stem cell homing are very likely inevitable when applied to porous biomimetic scaffolds being used as carriers for delivering stem cells [7]. Although MSCs acquired through bone marrow are beneficial for the previously mentioned reasons, the fact that myogenic lineage induction in MSCs is an additional differentiation step, as well as the fact that it is a relatively invasive procedure for the patient [52]. By comparison, skeletal muscles harbor their own endogenous organ-specific MSCs (satellite cells).…”

Section: Progenitor Cellsmentioning

confidence: 99%

“…By comparison, skeletal muscles harbor their own endogenous organ-specific MSCs (satellite cells). These satellite cells are already prone to myogenic differentiation and are more suitable for skeletal muscle TE as a result [52]. These are also very beneficial due to the fact that myogenic satellite cells start to proliferate in response to specific local challenges such as muscle damage [52].…”

Section: Progenitor Cellsmentioning

confidence: 99%

“…These satellite cells are already prone to myogenic differentiation and are more suitable for skeletal muscle TE as a result [52]. These are also very beneficial due to the fact that myogenic satellite cells start to proliferate in response to specific local challenges such as muscle damage [52]. The myogenic satellite cells migrate through the basal lamina sheets to the areas of injury and then differentiate into myoblasts and fuse with the preexisting damaged fibers or with each other in order to differentiate into muscle fibers.…”

Section: Progenitor Cellsmentioning

confidence: 99%

See 1 more Smart Citation

Cartilage and facial muscle tissue engineering and regeneration: a mini review

et al. 2018

View full text Add to dashboard Cite

Cartilage and facial muscle tissue provide basic yet vital functions for homeostasis throughout the body, making human survival and function highly dependent upon these somatic components. When cartilage and facial muscle tissues are harmed or completely destroyed due to disease, trauma, or any other degenerative process, homeostasis and basic body functions consequently become negatively affected. Although most cartilage and cells can regenerate themselves after any form of the aforementioned degenerative disease or trauma, the highly specific characteristics of facial muscles and the specific structures of the cells and tissues required for the proper function cannot be exactly replicated by the body itself. Thus, some form of cartilage and bone tissue engineering is necessary for proper regeneration and function. The use of progenitor cells for this purpose would be very beneficial due to their highly adaptable capabilities, as well as their ability to utilize a high diffusion rate, making them ideal for the specific nature and functions of cartilage and facial muscle tissue. Going along with this, once the progenitor cells are obtained, applying them to a scaffold within the oral cavity in the affected location allows them to adapt to the environment and create cartilage or facial muscle tissue that is specific to the form and function of the area. The principal function of the cartilage and tissue is vascularization, which requires a specific form that allows them to aid the proper flow of bodily functions related to the oral cavity such as oxygen flow and removal of waste. Facial muscle is also very thin, making its reproduction much more possible. Taking all these into consideration, this review aims to highlight and expand upon the primary benefits of the cartilage and facial muscle tissue engineering and regeneration, focusing on how these processes are performed outside of and within the body.

show abstract

“…This option is challenged by host muscle tissue availability and donor site morbidity such as functional loss and volume deficiency. 84,85 Recent advances in cell therapy using myoblasts have provided an alternate therapeutic opportunity to regenerate muscle tissue for functional augmentation. 86,87 Injection of cultured myoblasts has shown some efficacy; [88][89][90] however, this approach may not be suitable for treating large muscle injuries.…”

Section: Tissue Engineering Applicationsmentioning

confidence: 99%

Recent Applications of Polymeric Biomaterials and Stem Cells in Tissue Engineering and Regenerative Medicine

Lee

Yoo

Atala³

2014

Polymer Korea

View full text Add to dashboard Cite

Tissue engineering and regenerative medicine strategies could offer new hope for patients with serious tissue injuries or end-stage organ failure. Scientists are now applying the principles of cell transplantation, material science, and engineering to create biological substitutes that can restore and maintain normal function in diseased or injured tissues/ organs. Specifically, creation of engineered tissue construct requires a polymeric biomaterial scaffold that serves as a cell carrier, which would provide structural support until native tissue forms in vivo. Even though the requirements for scaffolds may be different depending on the target applications, a general function of scaffolds that need to be fulfilled is biodegradability, biological and mechanical properties, and temporal structural integrity. The scaffold's internal architecture should also enhance the permeability of nutrients and neovascularization. In addition, the stem cell field is advancing, and new discoveries in tissue engineering and regenerative medicine will lead to new therapeutic strategies. Although use of stem cells is still in the research phase, some therapies arising from tissue engineering endeavors that make use of autologous adult cells have already entered the clinic. This review discusses these tissue engineering and regenerative medicine strategies for various tissues and organs.

show abstract

Skeletal muscle tissue engineering

Cited by 263 publications

References 76 publications

Polyelectrolyte Multilayer Films of Controlled Stiffness Modulate Myoblast Cell Differentiation

Polyelectrolyte Multilayer Films of Controlled Stiffness Modulate Myoblast Cell Differentiation

Cartilage and facial muscle tissue engineering and regeneration: a mini review

Recent Applications of Polymeric Biomaterials and Stem Cells in Tissue Engineering and Regenerative Medicine

Contact Info

Product

Resources

About