Skeletal muscle targeting in vivo electroporation-mediated HGF gene therapy of bleomycin-induced pulmonary fibrosis in mice

The bleomycin animal model: A useful tool to investigate treatment options for idiopathic pulmonary fibrosis?

“…Li, Azuma, Takahashi et al, 2002) etc. ), Cytokines (Interferon-β (Azuma, Li et al, 2005), Interferon-γ (Gurujeyalakshmi & Giri, 1995;Hyde, Henderson, Giri, Tyler, & Stovall, 1988;Okada, Sugie, & Aisaka, 1993), Interleukin (IL)-1beta (M. Yasui et al, 1991), IL-10 (Arai et al, 2000), IL-18 (Nakatani-Okuda et al, 2005), Keratinocyte growth factor (Deterding et al, 1997;Sugahara, Iyama, Kuroda, & Sano, 1998;Yi et al, 1996), Hepatocyte growth factor (Dohi, Hasegawa, Yamamoto, & Marshall, 2000;Mizuno, Matsumoto, Li, & Nakamura, 2005;Umeda et al, 2004;Yaekashiwa et al, 1997), Chemokine ligand (CXCL)-10 (Tager et al, 2004),CXCL11 (Burdick et al, 2005), CD (cluster of differentiation)-36 (Yehualaeshet et al, 2000) etc. ), Cytokine antibodies (Transforming growth factor-β (Giri, Hyde, & Hollinger, 1993), Tumor necrosis factor-α (Fichtner-Feigl, Strober, Kawakami, Puri, & Kitani, 2006;Fujita et al, 2003;Piguet & Vesin, 1994), Connective tissue growth factor (Matsuoka et al, 2002), IL-12 , IL-13 (Fichtner-Feigl, Strober, Kawakami, Puri, & Kitani, 2006), Platelet derived growth factor (Aono et al, 2005;Chaudhary, Schnapp, & Park, 2006;Daniels et al, 2004;Yoshida et al, 1999), Vascular endothelial growth factor (Hamada et al, 2005), CCR-1 (Tokuda et al, 2000), CCR-3 (Huaux et al, 2005), CCL-11 (Huaux et al, 2005), CD-11 (Piguet, Rosen, Vesin, & Grau, 1993), MCP-1 etc.…”

“…Heme oxygenase 1 early (Tsuburai et al, 2002) 17 6 HGF early (Umeda et al, 2004) 17 7 SB 239063 early (Underwood et al, 2000) 17 8…”

Non-Viral Delivery Systems in Gene Therapy and Vaccine Development

“…In vivo use of electroporation is done by injecting naked DNA followed by electric pulses from electrodes that are located in situ in the target tissues. Successful use of electroporation was observed in transfecting muscles, brain, skin, liver, and tumors [Gardlik et al, 2005;Garcia-Frigola et al, 2007;Umeda et al, 2004;Babiuk et al, 2006;Harrison et al, 1998;Kang et al, 2011]. Since every tissue is specific and has its own characteristics, there are no generally accepted optimal conditions of electroporation that are suitable for effective transfection.…”

Paracrine factors from mesenchymal stem cells attenuate epithelial injury and lung fibrosis

“…Transplantation of alveolar epithelial cells, aimed at protecting cells from endogenous and exogenous injuries and stimulating regeneration, has been shown to ameliorate BLM-induced lung fibrosis (10). In addition, hepatocyte growth factor (HGF) and keratinocyte growth factor (KGF), which are both capable of promoting development and proliferation of AECs, can attenuate BLM-induced lung fibrosis (11)(12)(13). Therefore, any treatment capable of promoting proliferation or reducing apoptosis of AECs may ameliorate pulmonary fibrosis.…”