Skeletal muscle satellite cell populations in healthy young and older men and women

Roth, Stephen M.; Martel, Gregory F.; Ivey, Frederick M.; Lemmer, Jeffrey T.; Metter, E. Jeffrey; Hurley, Ben F.; Rogers, M. A.

doi:10.1002/1097-0185(200012)260:4<350::aid-ar30>3.0.co;2-6

Cited by 133 publications

(102 citation statements)

References 39 publications

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“…One of the initial hypotheses proposes a possible age-dependent decrease in the satellite cell pool, which may explain the reduced efficiency of muscle regeneration in older subjects (Renault et al, 2002a). However, this theory was disputed by reports showing that satellite cell proportion and morphology are generally conserved during the aging time-course, at least for the same muscle from healthy subjects (Roth et al, 2000b).…”

Section: Introductionmentioning

confidence: 99%

Molecular basis of the myogenic profile of aged human skeletal muscle satellite cells during differentiation

Pietrangelo¹,

Puglielli²,

Mancinelli³

et al. 2009

Experimental Gerontology

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ular basis of the myogenic profile of aged human skeletal muscle satellite cells during differentiation. Experimental Gerontology, Elsevier, 2009, 44 (8) This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 2 ACCEPTED MANUSCRIPT ACCEPTED MANUSCRIPT AbstractSarcopenia is the age-related loss of muscle mass, strength and function. Human muscle proteins are synthesized at a slower rate in the elderly than in young adults, leading to atrophy and muscle mass loss with a decline in the functional capability. Additionally, aging is accompanied by a decrease in the ability of muscle tissue to regenerate following injury or overuse due to the impairment of intervening satellite cells, in which we previously reported oxidative damage evidences. The aim of the present study was to determine the effects of aging on myoblasts and myotubes obtained from human skeletal muscle, and characterize the transcriptional profile as molecular expression patterns in relation to age-dependent modifications in their regenerative capacity. Our data show that the failure to differentiate does not depend on reduced myogenic cell number, but difficulty to complete the differentiation program. Data reported here suggested the following findings: i) oxidative damage accumulation in molecular substrates, probably due to impaired antioxidant activity and insufficient repair capability, ii) limited capability of elderly myoblasts to execute a complete differentiation program; restricted fusion, possibly due to altered cytoskeleton turnover and extracellular matrix degradation, and iii) activation of atrophy mechanism by activation of a specific FOXO-dependent program.

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Section: Introductionmentioning

confidence: 99%

Molecular basis of the myogenic profile of aged human skeletal muscle satellite cells during differentiation

Pietrangelo¹,

Puglielli²,

Mancinelli³

et al. 2009

Experimental Gerontology

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“…There is debate as to whether an age-related reduction in the number of SCs occurs, some works reporting a decrease in the SC number with aging (Brack et al 2005;Carlson et al 2009;Kadi et al 2004;Renault et al 2002a, b;Sajko et al 2004;Verdijk et al 2007) and some others reporting no agerelated changes (Brooks et al 2009;Dreyer et al 2006;Petrella et al 2006;Roth et al 2000). By contrast, there is consensus regarding an age-related decline in the performance of SCs as a primary cause of sarcopenia.…”

mentioning

confidence: 99%

Human muscle satellite cells show age-related differential expression of S100B protein and RAGE

Beccafico

Riuzzi

Puglielli

et al. 2010

AGE

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During aging, skeletal muscles show reduced mass and functional capacity largely due to loss of the regenerative ability of satellite cells (SCs), the quiescent stem cells located beneath the basal lamina surrounding each myofiber. While both the external environment and intrinsic properties of SCs appear to contribute to the age-related SC deficiency, the latter ones have been poorly investigated especially in humans. In the present work, we analyzed several parameters of SCs derived from biopsies of vastus lateralis muscle from healthy non-trained young (28.7±5.9 years; n=10) and aged (77.3±6.4 years; n= 11) people. Compared with young SCs, aged SCs showed impaired differentiation when cultured in differentiation medium, and exhibited the following: (1) reduced proliferation; (2) higher expression levels of S100B, a negative regulator of myoblast differentiation; (3) undetectable levels in growth medium of fulllength RAGE (receptor for advanced glycation end products), a multiligand receptor of the immunoglobulin superfamily, the engagement of which enhances myoblast differentiation; and (4) lower expression levels of the transcription factors, MyoD and Pax7. Also, either overexpression of full-length RAGE or knockdown of S100B in aged SCs resulted in enhanced differentiation, while overexpression of either a nontransducing mutant of RAGE (RAGEΔcyto) or S100B in young SCs resulted in reduced differentiation compared with controls. Moreover, while aged SCs maintained the ability to respond to mitogenic factors (e.g., bFGF and S100B), they were no longer able to secrete these factors, unlike young SCs. These data support a role for intrinsic factors, besides the extracellular environment in the defective SC function in aged skeletal muscles.

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“…During the ageing process, satellite cells may decrease in number [35][36][37]. Other studies found no reduction in satellite cell numbers in ageing skeletal muscle [27,38]. Similar to aged myonuclei, it appears that satellite cells have a decreased functional ability [39,40].…”

Section: Myonuclear Domain and Atrophy/sarcopeniamentioning

confidence: 88%

Prevention of Skeletal Muscle Wasting: Disuse Atrophy and Sarcopenia

Brooks¹,

Myburgh²

2012

Skeletal Muscle - From Myogenesis to Clinical Relations

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Skeletal muscle satellite cell populations in healthy young and older men and women

Cited by 133 publications

References 39 publications

Molecular basis of the myogenic profile of aged human skeletal muscle satellite cells during differentiation

Molecular basis of the myogenic profile of aged human skeletal muscle satellite cells during differentiation

Human muscle satellite cells show age-related differential expression of S100B protein and RAGE

Prevention of Skeletal Muscle Wasting: Disuse Atrophy and Sarcopenia

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