Skeletal Muscle Regenerative Engineering

Tang, Xiaoyan; Daneshmandi, Leila; Awale, Guleid; Nair, Lakshmi S.; Laurencin, Cato T.

doi:10.1007/s40883-019-00102-9

Cited by 26 publications

(18 citation statements)

References 194 publications

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“…Regenerative Engineering is defined as the Convergence of Advanced Materials Sciences, Stem Cell Sciences, Physics, Developmental Biology, and Clinical Translation for the regeneration of complex tissues and organ systems (Laurencin & Khan, 2012;Laurencin & Nair, 2016;Tang, Daneshmandi, Awale, Nair, & Laurencin, 2019). At the forefront of this transdisciplinary approach is the study of epimorphic regeneration and understanding the morphogenetic cues involved in a regenerative limb to help develop translational strategies for tissue and organ regeneration (Laurencin & Nair, 2016;Stoick-Cooper, Moon, & Weidinger, 2007).…”

Section: Discussionmentioning

confidence: 99%

Regenerative engineered vascularized bone mediated by calcium peroxide

Daneshmandi

Laurencin

2020

J Biomedical Materials Res

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One of the main challenges hindering the clinical translation of bone tissue engineering scaffolds is the lack of establishment of functional vasculature. Insufficient vascularization and poor oxygen supply limit cell survival within the constructs resulting in poor osseointegration with the host tissue and eventually leading to inadequate bone regeneration. Inspired by cues from developmental biology, we regenerative engineered a composite matrix by incorporating calcium peroxide (CaO2) into poly(lactide‐co‐glycolide) (PLGA) microsphere‐based matrices and sought to assess whether the delivery of the byproducts of CaO2 decomposition, namely O2, Ca2+, and H2O2 could enhance the regeneration of vascularized bone tissue. The composite microspheres were successfully fabricated via the oil‐in‐water emulsion method. The presence and encapsulation of CaO2 was confirmed using scanning electron microscopy, energy dispersive x‐ray spectroscopy, thermogravimetric analysis, and X‐ray powder diffraction. The microspheres were further heat sintered into three‐dimensional porous scaffolds and characterized for their degradation and release of byproducts. The in vitro cytocompatibility of the matrices and their ability to support osteogenic differentiation was confirmed using human adipose‐derived stem cells. Lastly, an in vivo study was performed in a mouse critical‐sized calvarial defect model to evaluate the capacity of these matrices in supporting vascularized bone regeneration. Results demonstrated that the presence of CaO2 increased cellularization and biological activity throughout the matrices. There was greater migration of host cells to the interior of the matrices and greater survival and persistence of donor cells after 8 weeks, which in synergy with the composite matrices led to enhanced vascularized bone regeneration.

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Section: Discussionmentioning

confidence: 99%

Regenerative engineered vascularized bone mediated by calcium peroxide

Daneshmandi

Laurencin

2020

J Biomedical Materials Res

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“…In this regard, tremendous efforts in the research fields of tissue engineering and regenerative medicine have been devoted to addressing the unmet medical needs for artificial organs and tissues 134 . Regenerative medicine or tissue engineering is a multidisciplinary field that is based on the convergent technologies of materials science, biology, stem cells technology, and clinical translation to regenerate and/or restore damaged tissues as a consequence of disease, congenital abnormalities, or trauma 135 . Engineering functional organs or tissues is a complicated process, which starts with a rational design of scaffold/implant biomaterials 136 .…”

Section: Materdicine In Regeneration Medicinementioning

confidence: 99%

Materdicine: Interdiscipline of materials and medicine

Xiang

Chen

2020

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The clinical medicine and biomaterials are two fields that are conducive to achieve the goal of precise medicine on diagnostics and therapeutics of various diseases. The interdiscipline of materials and medicine, termed/abbreviated as “materdicine,” seeks to address the dominant medical shortcomings and challenges faced by conventional medicine, including inferior bioavailability, systemic toxicity, poor targeting specificity, and unsatisfied diagnostic/therapeutic efficacy. In this review, we present the perspective and discussion on the use of diverse biomaterials for disease diagnosis and treatment from a broad perspective, especially on nanoscale biomaterials. We initially highlight the recent advances on the engineering of abundant contrast agents for diagnostic bioimaging, such as optical fluorescence imaging, magnetic resonance imaging, and photoacoustic imaging. In addition, discussions on the diagnostic biosensing for point‐of‐care diagnosis, such as fluorescent and plasmonic sensors, are supplemented. Furthermore, we outline several materdicine‐enabled therapeutic modalities for disease treatments, including the following exemplified scaffold biomaterials for regenerative medicine. Especially, rational modulation of toxicity issues of micro‐/nanoscale biomaterials is also accounted for addressing the possible concerns of biocompatibility and biosafety on further clinical translation of materdicine. Finally, we summarize facing challenges and outlook future developments relating to the clinical translation of these distinctive biomaterials in materdicine.

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“…To develop strategies to regenerate complex tissues in mammals, and a limb one day, regenerative engineering approaches will be needed ( Laurencin and Khan, 2012 ; Laurencin and Nair, 2016 ). Regenerative engineering is a convergence field whereby advanced material science, physical science, stem cell science, developmental biology and translational medicine with the goal of creating tools that regenerate or reconstruct functional tissues and organs ( Ameer, 2020 ; Beachy et al, 2020 ; Bowers and Brown, 2019 ; Clegg et al, 2019 ; Heath, 2019 ; Ifegwu et al, 2018 ; James et al, 2016 ; Mengsteab et al, 2020 ; Moore and West, 2019 ; Nelson et al, 2018 ; Ogueri et al, 2019 ; Tang et al, 2019 , 2020 ). As a part of this approach, developmental and regeneration biology will provide clues that can direct new engineering strategies for coordinated regeneration of tissues with limited regeneration capacity.…”

Section: Introductionmentioning

confidence: 99%

Control of mesenchymal cell fate via application of FGF-8b in vitro

2021

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In order to develop strategies to regenerate complex tissues in mammals, understanding the role of signaling in regeneration competent species and mammalian development is of critical importance. Fibroblast growth factor 8 (FGF-8) signaling has an essential role in limb morphogenesis and blastema outgrowth. Therefore, we aimed to study the effect of FGF-8b on the proliferation and differentiation of mesenchymal stem cells (MSCs), which have tremendous potential for therapeutic use of cell-based therapy. Rat adipose derived stem cells (ADSCs) and muscle progenitor cells (MPCs) were isolated and cultured in growth medium and various types of differentiation medium (osteogenic, chondrogenic, adipogenic, tenogenic, and myogenic medium) with or without FGF-8b supplementation. We found that FGF-8b induced robust proliferation regardless of culture medium. Genes related to limb development were upregulated in ADSCs by FGF-8b supplementation. Moreover, FGF-8b enhanced chondrogenic differentiation and suppressed adipogenic and tenogenic differentiation in ADSCs. Osteogenic differentiation was not affected by FGF-8b supplementation. FGF-8b was found to enhance myofiber formation in rat MPCs. Overall, this study provides foundational knowledge on the effect of FGF-8b in the proliferation and fate determination of MSCs and provides insight in its potential efficacy for musculoskeletal therapies.

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Skeletal Muscle Regenerative Engineering

Cited by 26 publications

References 194 publications

Regenerative engineered vascularized bone mediated by calcium peroxide

Regenerative engineered vascularized bone mediated by calcium peroxide

Materdicine: Interdiscipline of materials and medicine

Control of mesenchymal cell fate via application of FGF-8b in vitro

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