Skeletal muscle protein accretion rates and hindlimb growth are reduced in late gestation intrauterine growth‐restricted fetal sheep

Abstract

Key pointsr Adults who were affected by intrauterine growth restriction (IUGR) suffer from reductions in muscle mass, which may contribute to insulin resistance and the development of diabetes.r We demonstrate slower hindlimb linear growth and muscle protein synthesis rates that match the reduced hindlimb blood flow and oxygen consumption rates in IUGR fetal sheep. These adaptations resulted in hindlimb blood flow rates in IUGR that were similar to control fetuses on a weight-specific basis.r Net hindlimb glucose uptake and lactate output rates were similar between groups, whereas amino acid uptake was significantly lower in IUGR fetal sheep.r Among all fetuses, blood O 2 saturation and plasma glucose, insulin and insulin-like growth factor-1 were positively associated and norepinephrine was negatively associated with hindlimb weight.r These results further our understanding of the metabolic and hormonal adaptations to reduced oxygen and nutrient supply with placental insufficiency that develop to slow hindlimb growth and muscle protein accretion.Abstract Reduced skeletal muscle mass in the fetus with intrauterine growth restriction (IUGR) persists into adulthood and may contribute to increased metabolic disease risk. To determine how placental insufficiency with reduced oxygen and nutrient supply to the fetus affects hindlimb blood flow, substrate uptake and protein accretion rates in skeletal muscle, late gestation control (CON) (n = 8) and IUGR (n = 13) fetal sheep were catheterized with aortic and femoral catheters and a flow transducer around the external iliac artery. Muscle protein kinetic rates were measured using isotopic tracers. Hindlimb weight, linear growth rate, muscle protein accretion rate and fractional synthetic rate were lower in IUGR compared to CON (P < 0.05). Absolute hindlimb blood flow was reduced in IUGR (IUGR: 32.9 ± 5.6 ml min −1 ; CON: 60.9 ± 6.5 ml min −1 ; P < 0.005), although flow normalized to hindlimb weight was similar between groups. Hindlimb oxygen consumption rate was lower in IUGR (IUGR: 10.4 ± 1.4 μmol min −1 100 g −1 ; CON: 14.7 ± 1.3 μmol min −1 100 g −1 ; P < 0.05). Hindlimb glucose uptake and lactate output rates were similar between groups, whereas amino acid uptake was lower in IUGR (IUGR: 1.3 ± 0.5 μmol min −1 100 g −1 ; CON: 2.9 ± 0.2 μmol min −1 100 g −1 ; P < 0.05). Blood O 2 saturation (r 2 = 0.80, P < 0.0001) and plasma glucose (r 2 = 0.68, P < 0.0001), insulin (r 2 = 0.40, P < 0.005) and insulin-like growth factor (IGF)-1 (r 2 = 0.80, P < 0.0001) were positively associated and norepinephrine (r 2 = 0.59, P < 0.0001) was negatively associated with hindlimb weight. Slower hindlimb linear growth and muscle protein synthesis rates match reduced hindlimb blood flow and oxygen consumption rates in the IUGR fetus. Metabolic adaptations to slow hindlimb growth are probably hormonally-mediated by mechanisms that include increased fetal norepinephrine and reduced IGF-1 and insulin.

Postnatal β2 adrenergic treatment improves insulin sensitivity in lambs with IUGR but not persistent defects in pancreatic islets or skeletal muscle

“…Equivalent lactate output, nutrient quotients and skeletal muscle glycogen content among all of our lambs indicates that the lower fractional glucose oxidation was not offset by greater glycolytic rates and glucose storage. Hindlimb glucose and lactate fluxes in IUGR fetal sheep further indicate that faster glycolytic rates are not a component of IUGR skeletal muscle programming (Rozance et al 2018). Thus, additional studies will be needed to characterize the mechanisms responsible for the programmed defects in skeletal muscle glucose metabolism demonstrated by this study.…”

“…Hindlimb glucose and lactate fluxes in IUGR fetal sheep further indicate that faster glycolytic rates are not a component of IUGR skeletal muscle programming (Rozance et al . ). Thus, additional studies will be needed to characterize the mechanisms responsible for the programmed defects in skeletal muscle glucose metabolism demonstrated by this study.…”

“…Mean hindlimb glucose, lactate, [ 14 C]glucose and 14 CO 2 fluxes were calculated from the simultaneously collected femoral arterial and venous blood sample pairs. Weight-specific net uptake rates of oxygen, [ 14 C]glucose and glucose as well as outputs of lactate were calculated by the Fick principle as the product of artery blood flow and arteriovenous difference (Rozance et al 2018). Arterial blood [ 14 C]glucose concentrations (d.p.m.…”

Impact of thermal stress on placental function and fetal physiology

“…Mean plasma insulin (A), glucagon (B), norepinephrine (C), cortisol (D), and insulin-like growth factor I (IGF-I, E) concentrations are presented for fetuses at 90% of gestation. Group means for thermoneutral control and heat stressed ewes were reported in Limesand et al, 2006;Brown et al, 2012Brown et al, , 2016Thorn et al, 2013;Macko et al, 2016;and Rozance et al, 2018. An ANOVA with reported study as the repeated measure identifies group (control and heat stress) differences (P-values) for hormone concentrations.…”

Messing with metabolism: lessons from an IUGR fetus

“…As highlighted in this issue of The Journal of Physiology , maternal–fetal hypoperfusion necessitates a hard choice: the sparing of critical fetal organs by limiting nutrient/O 2 delivery and utilisation by non‐essential fetal tissue beds (Rozance et al . ).…”