Skeletal muscle Kv7 (KCNQ) channels in myoblast differentiation and proliferation

Roura-Ferrer, Meritxell; Solé, Laura; Martínez-Mármol, Ramón; Villalonga, Núria; Felipe, Antônio

doi:10.1016/j.bbrc.2008.02.152

Cited by 39 publications

(49 citation statements)

References 27 publications

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“…In addition, K v 7.1 (Tsevi et al, 2005) and K v 7.5 (Lerche et al, 2000;Schroeder et al, 2000) transcripts have been detected in adult skeletal muscle; changes in K v 7.1 and K v 7.5 transcript levels occur during proliferation and differentiation of rat L6E9 myoblasts in vitro (Roura-Ferrer et al, 2008). However, no data are yet available on other K v 7 family members, and the functional role of K v 7.1 and K v 7.5 in skeletal muscle is still poorly defined.…”

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confidence: 97%

Expression, Localization, and Pharmacological Role of K_v7 Potassium Channels in Skeletal Muscle Proliferation, Differentiation, and Survival after Myotoxic Insults

Iannotti

Panza²,

Barrese³

et al. 2009

J Pharmacol Exp Ther

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confidence: 97%

Expression, Localization, and Pharmacological Role of K_v7 Potassium Channels in Skeletal Muscle Proliferation, Differentiation, and Survival after Myotoxic Insults

Iannotti

Panza²,

Barrese³

et al. 2009

J Pharmacol Exp Ther

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“…5 However, Kv7.1 and Kv7.5 are coexpressed in smooth and skeletal muscle cells. 2,6,20,21 To date, there are no reports on the association of Kv7.1 and Kv7.5, which could have important influences in vascular reactivity.…”

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confidence: 99%

Functional Assembly of Kv7.1/Kv7.5 Channels With Emerging Properties on Vascular Muscle Physiology

Oliveras

Roura-Ferrer

Solé

et al. 2014

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Objective— Voltage-dependent K + (Kv) channels from the Kv7 family are expressed in blood vessels and contribute to cardiovascular physiology. Although Kv7 channel blockers trigger muscle contractions, Kv7 activators act as vasorelaxants. Kv7.1 and Kv7.5 are expressed in many vessels. Kv7.1 is under intense investigation because Kv7.1 blockers fail to modulate smooth muscle reactivity. In this study, we analyzed whether Kv7.1 and Kv7.5 may form functional heterotetrameric channels increasing the channel diversity in vascular smooth muscles. Approach and Results— Kv7.1 and Kv7.5 currents elicited in arterial myocytes, oocyte, and mammalian expression systems suggest the formation of heterotetrameric complexes. Kv7.1/Kv7.5 heteromers, exhibiting different pharmacological characteristics, participate in the arterial tone. Kv7.1/Kv7.5 associations were confirmed by coimmunoprecipitation, fluorescence resonance energy transfer, and fluorescence recovery after photobleaching experiments. Kv7.1/Kv7.5 heterotetramers were highly retained at the endoplasmic reticulum. Studies in HEK-293 cells, heart, brain, and smooth and skeletal muscles demonstrated that the predominant presence of Kv7.5 stimulates release of Kv7.1/Kv7.5 oligomers out of lipid raft microdomains. Electrophysiological studies supported that KCNE1 and KCNE3 regulatory subunits further increased the channel diversity. Finally, the analysis of rat isolated myocytes and human blood vessels demonstrated that Kv7.1 and Kv7.5 exhibited a differential expression, which may lead to channel diversity. Conclusions— Kv7.1 and Kv7.5 form heterotetrameric channels increasing the diversity of structures which fine-tune blood vessel reactivity. Because the lipid raft localization of ion channels is crucial for cardiovascular physiology, Kv7.1/Kv7.5 heteromers provide efficient spatial and temporal regulation of smooth muscle function. Our results shed light on the debate about the contribution of Kv7 channels to vasoconstriction and hypertension.

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“…21 K V 7 channels are also important in nonexcitable cells, such as epithelia, [22][23][24] and in regulation of cell volume 25 and ion transport (Cl Ϫ and Na ϩ ) across epithelia. 22,23 In skeletal muscle myoblasts, KCNQ5 has been implicated in cell-cycle progression and cellular proliferation and differentiation 26 ; but this appears to have been less well studied in vascular smooth muscle. Voltage-dependant potassium channel dysfunction has been linked previously to abnormalities in proliferation and possibly also to remodeling.…”

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confidence: 99%

Novel Expression and Regulation of Voltage-Dependent Potassium Channels in Placentas From Women With Preeclampsia

et al. 2011

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Abstract-Preeclampsia is associated with structural/functional alterations in placental and maternal vasculature.Voltage-dependant potassium channels encoded by KCNQ1-5 genes have been detected in several types of blood vessels where they promote vascular relaxation. Voltage-dependant potassium channel function can be modulated by KCNE1-5-encoded accessory proteins. The aim of this study was to determine whether KCNQ and KCNE genes are differentially expressed in placentas from women with preeclampsia compared with normotensive controls and to examine any differences in those who delivered preterm (Ͻ37 weeks) or term. Key Words: potassium channel Ⅲ placenta Ⅲ preeclampsia Ⅲ KCNQ Ⅲ KCNE P reeclampsia is a pregnancy-specific condition affecting 2% to 7% of women and is associated with maternal multiorgan dysfunction. 1 This disorder, which is responsible for Ϸ60 000 maternal deaths each year worldwide, 2 increases perinatal mortality 5-fold 3 and is commonly associated with preterm delivery and fetal growth restriction. 4 Women who develop preeclampsia and their infants are at increased risk of hypertension, metabolic disorders, cardiovascular disease, and cardiovascular death in later life. 5,6 The pathophysiology underpinning the disorder is complex. Impaired placentation almost certainly plays a part. Placental and maternal oxidative stress and generalized systemic inflammatory activation 7 are main components of the syndrome. 8 Altered maternal and placental vascular reactivity and endothelial cell function have been implicated in the clinical manifestations of preeclampsia, for example, an increase in total peripheral vascular resistance, 9,10 hypertension, and altered hemodynamics.There is emerging evidence to suggest that potassium channels play important roles in the feto-placental vasculature. 11-15 Specifically, it is proposed that suppression of voltage-dependant potassium channel function may increase vascular tone and, hence, be a mechanism affecting perfusion in placentas from women with preeclampsia.The subfamilies of voltage-gated K V 7 channels (potassium channel complexes encoded by KCNQ1-5 and KCNE1-5 genes) are of particular interest, because preliminary data indicate the presence of functional K V 7 channels in human chorionic plate arteries. 15 K V 7 channel activity is generally associated with outwardly rectifying, voltage-dependent K ϩ

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Skeletal muscle Kv7 (KCNQ) channels in myoblast differentiation and proliferation

Cited by 39 publications

References 27 publications

Expression, Localization, and Pharmacological Role of K_v7 Potassium Channels in Skeletal Muscle Proliferation, Differentiation, and Survival after Myotoxic Insults

Expression, Localization, and Pharmacological Role of K_v7 Potassium Channels in Skeletal Muscle Proliferation, Differentiation, and Survival after Myotoxic Insults

Functional Assembly of Kv7.1/Kv7.5 Channels With Emerging Properties on Vascular Muscle Physiology

Novel Expression and Regulation of Voltage-Dependent Potassium Channels in Placentas From Women With Preeclampsia

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Skeletal muscle Kv7 (KCNQ) channels in myoblast differentiation and proliferation

Cited by 39 publications

References 27 publications

Expression, Localization, and Pharmacological Role of Kv7 Potassium Channels in Skeletal Muscle Proliferation, Differentiation, and Survival after Myotoxic Insults

Expression, Localization, and Pharmacological Role of Kv7 Potassium Channels in Skeletal Muscle Proliferation, Differentiation, and Survival after Myotoxic Insults

Functional Assembly of Kv7.1/Kv7.5 Channels With Emerging Properties on Vascular Muscle Physiology

Novel Expression and Regulation of Voltage-Dependent Potassium Channels in Placentas From Women With Preeclampsia

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Expression, Localization, and Pharmacological Role of K_v7 Potassium Channels in Skeletal Muscle Proliferation, Differentiation, and Survival after Myotoxic Insults

Expression, Localization, and Pharmacological Role of K_v7 Potassium Channels in Skeletal Muscle Proliferation, Differentiation, and Survival after Myotoxic Insults