Skeletal Dysmorphology in Fetus Following Colloidal Silver Ingestion in Pregnant Swiss Albino Mice

Abstract

Among all nanoparticles Silver nanoparticle is important because of its antifungal & antimicrobial activity and also known to be anti-inflammatory and wound healer. Silver nanoparticle is a strong bactericidal agent as it cleaves silver ion of atomic valency one inside the bacterial cell. Silver nanoparticle in moderate manner causes neurotoxicity, skeletal dysmorphogenesis and embryo teratogenicity in in-vitro position of model mouse. 20 to 1908 nm size silver nanoparticles in colloidal solution was given in dose (5, 10, 15, 20 mg/kg b.w.) respectively to randomly chosen mother mice from the colony by oral gavages from 7 th to 12 th day of gestational age. The control group mother received normal saline. Fetuses were collected on day 18 th of gestation. Alizarin staining was done to evaluate the skeletal dysmorphology. The common skeletal dysmorphology seen were unossification of sternum, frontal bone, parietal bone, temporal bone, mandible, maxilla, vertebral column, scapulae and ribs along with those cleft, scoliosis, spina bifida and hypoplasia was also seen.