Sizing the ends: Normal length of human telomeres

Samassékou, Oumar; Gadji, Macoura; Drouin, Régen; Yan, Jun

doi:10.1016/j.aanat.2010.07.005

Cited by 104 publications

(100 citation statements)

References 124 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…There are various methods established to determine telomere length like telomere restriction fragment (TRF) analysis, quantitative PCR, and the single telomere length analysis (STELA) [for review: (57)]. More sensitive methods include fluorescence in situ hybridization (FISH) (54) and the primed in situ (PRINS) labeling technique (58,59) allowing measurement of telomere lengths.…”

Section: Discussionmentioning

confidence: 99%

Novel automated three‐dimensional genome scanning based on the nuclear architecture of telomeres

Klewes

Höbsch

Katzir³

et al. 2010

Cytometry Pt A

View full text Add to dashboard Cite

Telomeres, the end of chromosomes, are organized in a nonoverlapping fashion and form microterritories in nuclei of normal cells. Previous studies have shown that normal and tumor cell nuclei differ in their 3D telomeric organization. The differences include a change in the spatial organization of the telomeres, in telomere numbers and sizes and in the presence of telomeric aggregates. Previous attempts to identify the above parameters of 3D telomere organization were semi-automated. Here we describe the automation of 3D scanning for telomere signatures in interphase nuclei based on three-dimensional fluorescent in situ hybridization (3D-FISH) and, for the first time, define its sensitivity in tumor cell detection. The data were acquired with a highthroughput scanning/acquisition system that allows to measure cells and acquire 3D images of nuclei at high resolution with 403 or 603 oil and at a speed of 10,000-15,000 cells h 21 , depending on the cell density on the slides. The automated scanning, TeloScan, is suitable for large series of samples and sample sizes. We define the sensitivity of this automation for tumor cell detection. The data output includes 3D telomere positions, numbers of telomeric aggregates, telomere numbers, and telomere signal intensities. We were able to detect one aberrant cell in 1,000 normal cells. In conclusions, we are able to detect tumor cells based on 3D architectural profiles of the genome. This new tool could, in the future, assist in patient diagnosis, in the detection of minimal residual disease, in the analysis of treatment response and in treatment decisions. '

show abstract

Section: Discussionmentioning

confidence: 99%

Novel automated three‐dimensional genome scanning based on the nuclear architecture of telomeres

Klewes

Höbsch

Katzir³

et al. 2010

Cytometry Pt A

View full text Add to dashboard Cite

show abstract

“…STELA, a common method for age estimation, is used to measure the telomere length of specific chromosomes, especially those with shorter telomeres. This method was originally developed to measure XpYp has also been successfully used to size the following chromosomes: 2p, 7q, 11q, 12q, 16q, 16p, and17p [1,26,27]. Age estimation using STELA (for the XpYp telomere) was also unsuccessful because there was inconsistent amplification among the samples (n = 16) and the biological age of the sample donors did not seem to correlate with the resulting amplified telomere lengths [1].…”

Section: Age and Telomere Lengthmentioning

confidence: 99%

Exploring the utility of genetic markers for predicting biological age

Saeed¹,

Berlin²,

Cruz³

2012

Legal Medicine

View full text Add to dashboard Cite

“…Once TL becomes too short, telomeres trigger the onset of cellular senescence (Carulli and 25 Annicchiarico, 2014). Therefore, TL is critically associated with the remaining proliferative capacity of a cell (Samassekou et al, 2010). On the other hand, numerous cell types such as stem cells and activated lymphocytes, have the ability to resynthesize telomeric repeats primarily via the complexly regulated enzyme telomerase (Weng, 2001).…”

Section: Introduction and Scopementioning

confidence: 99%

“…However, as in other eukaryotic organisms, chromosomes in humans usually consist of a variable number of such repeats and hence of variable TL values, respectively (Samassekou et al, 2010).…”

Section: Introduction and Scopementioning

confidence: 99%

See 1 more Smart Citation

The influence of physical exercise and sports on telomere length - A model for telomere length and telomerase activity regulation based on a comparative assessment of literature

Fröhlich

2016

Preprint

View full text Add to dashboard Cite

Based upon a comprehensive analysis of current literature and by combining a molecular biology and a sports science perspective, this review examines (1) if a correlation between physical activity load and telomere length (TL) exists, and (2) comprehensively analyses and integrates molecular pathways regulating exercise dependent TL dynamics. The focus is on TL in leukocytes and muscle tissue in middle to advanced aged subjects. Regarding item (1), a strong tendency for an increase in mean leukocyte TL was found for exercise energy expenditures up to about 2∙103 kcal/week, while for higher activity levels no conclusive statement can be made. Conversely, research on skeletal muscle TL so far is quite limited but suggests that physical exercise with prolonged eccentric muscle contractions rather acts to shorten telomeres, while sports with little eccentric contractions might rather act to lengthen telomeres. As to item (2), a model for hypothetical pathways for exercise dependent telomerase activity regulation is proposed by consolidating findings of different studies in different cells. Consistent with this pathway model, various studies report increased telomerase transcription or activation by exercise. Moreover, a qualitative overall model for endurance exercise related TL dynamics is presented. It considers telomeres as dynamic structures in equilibrium between telomere shortening (e.g., cellular turnover, oxidative stress, inflammation) and telomere lengthening (e.g., telomerase activity, telomerase recruitment) effects. A negative feedback-loop mediated by enhanced telomerase recruitment to short telomeres is assumed to counteract too excessive TL alterations. Finally, a proposal is put forth for future research on exercise dependent telomere dynamics by adopting a systems biology approach to develop mathematical models that properly integrate the complexity of the interacting variables.

show abstract

Sizing the ends: Normal length of human telomeres

Cited by 104 publications

References 124 publications

Novel automated three‐dimensional genome scanning based on the nuclear architecture of telomeres

Novel automated three‐dimensional genome scanning based on the nuclear architecture of telomeres

Exploring the utility of genetic markers for predicting biological age

The influence of physical exercise and sports on telomere length - A model for telomere length and telomerase activity regulation based on a comparative assessment of literature

Contact Info

Product

Resources

About