Size-tunable protein–polymer hybrid carrier for cell internalization

Lee, Chaeyeon; Choi, Ji Eun; Park, Gil Yong; Lee, Taeheon; Kim, Jinku; An, Seong Soo A.; Song, Jae Kwang; Paik, Hyun‐jong

doi:10.1016/j.reactfunctpolym.2018.01.006

Cited by 8 publications

(7 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This may be due to the formation of polymer–protein hybrids (Figure S12). − To quantify the extent of aggregation and protection and to investigate any changes in the higher-order structure of proteins on aggregation, further studies were conducted.…”

Section: Results and Discussionmentioning

confidence: 99%

Dual Thermo- and pH-Responsive Behavior of Double Zwitterionic Graft Copolymers for Suppression of Protein Aggregation and Protein Release

Zhao

Rajan

Matsumura

2019

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Graft copolymers consisting of two different zwitterionic blocks were synthesized via reversible addition fragmentation chain transfer polymerization. These polymers showed dual properties of thermo- and pH-responsiveness in an aqueous solution. Ultraviolet–visible spectroscopy and dynamic light scattering were employed to study the phase behavior under varying temperatures and pH values. Unlike the phase transition temperatures of other graft copolymers containing nonionic blocks, the phase transition temperature of these polymers was easily tuned by changing the polymer concentration. Owing to the biocompatible and stimuli-responsive nature of the polymers, this system was shown to effectively release proteins (lysozyme) while simultaneously protecting them against denaturation. The positively charged lysozyme was shown to bind with the negatively charged polymer at the physiological pH (pH 7.4). However, it was subsequently released at pH 3, at which the polymer exhibits a positive charge. Protein aggregation studies using a residual enzymatic activity assay, circular dichroism, and a Thioflavin T assay revealed that the secondary structure of the lysozyme was retained even after harsh thermal treatment. The addition of these polymers helped the lysozyme retain its enzymatic activity and suppressed its fibrillation. Both polymers showed excellent protein protection properties, with the negatively charged polymer exhibiting slightly superior protein protection properties to those of the neutral polymer. To the best of the authors’ knowledge, this is the first study to develop a graft copolymer system consisting of two different zwitterionic blocks that shows dual thermo- and pH-responsive properties. The presence of the polyampholyte structure enables these polymers to act as protein release agents, while simultaneously protecting the proteins from severe stress.

show abstract

Section: Results and Discussionmentioning

confidence: 99%

Dual Thermo- and pH-Responsive Behavior of Double Zwitterionic Graft Copolymers for Suppression of Protein Aggregation and Protein Release

Zhao

Rajan

Matsumura

2019

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…The size of a PTPNB can be controlled by adjusting the extent of aggregation. 29,30 Thus, the size of GFP/PLAs can be controlled by adjusting the amount of polymer. Ni 2+ NTA-PLA solutions in DMF were prepared at different concentrations (1 and 3 mg/mL, previous concentration: 2 mg/mL).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…This research builds on the polymer-templated protein nanoball (PTPNB) system described in previous reports. − The PTPNB system is a size-controllable method of preparing polymer–protein core–shell nanoparticles. This system is based on the specific interactions of NTA-Ni 2+ -His with polymer chain ends and multihistidine tags on proteins.…”

Section: Resultsmentioning

confidence: 99%

Orientation Controlled Protein Nanocapsules by Enzymatic Removal of a Polymer Template

et al. 2018

Self Cite

View full text Add to dashboard Cite

Protein nanocapsules are potentially useful as functional nanocarriers because of their hollow structure and high biocompatibility and the intrinsic activity of their protein constituents. However, the development of a facile method for the preparation of oriented nanocapsules that retain their protein activity has been challenging. Here we describe the preparation of protein nanocapsules through the enzymatic removal of polymer templates. Nickel(II) nitrilotriacetic acid-end-functionalized poly(lactic acid) (Ni2+-NTA-PLA) was introduced as a polymeric template to immobilize hexa-histidine-tagged green fluorescence protein (His6-GFP) with consistent orientation. Following protein cross-linking and core-degradation, various measurements as a function of degradation time indicated the formation of hollow structures. We also demonstrated orientational control and activity preservation of the protein after capsule preparation. Protein nanocapsules prepared by this method can act as functional containers, taking advantage of the intrinsic function of their constituent proteins without additional modification.

show abstract

“…35 This process improves the selectivity between Ni-NTA on the surface of HNPs and Histagged FimH via specific NTA-Ni 2+ -His interactions. 38 In addition, the efficiency of ICG incorporation and its stability were enhanced as the HNP was coated with a lipid layer on the PLGA polymer core. Collectively, HNPs can function as multifunctional nanoparticles through the incorporation of hydrophobic drugs into the PLGA core and the decoration of proteins in the lipid shell.…”

Section: Open Accessmentioning

confidence: 99%

Escherichia coliadhesin protein-conjugated thermal responsive hybrid nanoparticles for photothermal and immunotherapy against cancer and its metastasis

Hwang

Zhang

Park

et al. 2021

J Immunother Cancer

View full text Add to dashboard Cite

BackgroundAdvanced cancer therapy is targeted at primary tumors and also recurrent or metastatic cancers. Combinational cancer treatment has recently shown high efficiency against recurrent and metastatic cancers. In this study, we synthesized a thermal responsive hybrid nanoparticle (TRH) containing FimH, an immune stimulatory recombinant protein, for the induction of a combination of photothermal therapy (PTT) and immunotherapy against cancer and its metastasis.MethodsThe hybrid nanoparticle was incorporated with a near-infrared (NIR) absorbent, indocyanine green, and decorated with FimH on its surface to form F-TRH. F-TRH was evaluated for its anticancer and antimetastatic effects against CT-26 carcinoma in mice by combining PTT and immunotherapy.ResultsNIR laser irradiation elicited an elevation of temperature in F-TRH, which induced apoptosis in CT-26 carcinoma cells in vitro. In addition, F-TRH and NIR laser irradiation promoted photothermal-mediated therapeutic effects against CT-26 and 4T1 tumors in mice. The release of FimH from F-TRH in response to elevated temperature and apoptotic bodies of cancer cells via PTT elicited dendritic cell-mediated cancer antigen-specific T-cell responses, which subsequently inhibited the second challenge of CT-26 and 4T1 cell growth in the lung.ConclusionsThese data demonstrate the potential use of F-TRH for immuno-photothermal therapy against cancer and its recurrence and metastasis.

show abstract

Size-tunable protein–polymer hybrid carrier for cell internalization

Cited by 8 publications

References 32 publications

Dual Thermo- and pH-Responsive Behavior of Double Zwitterionic Graft Copolymers for Suppression of Protein Aggregation and Protein Release

Dual Thermo- and pH-Responsive Behavior of Double Zwitterionic Graft Copolymers for Suppression of Protein Aggregation and Protein Release

Orientation Controlled Protein Nanocapsules by Enzymatic Removal of a Polymer Template

Escherichia coliadhesin protein-conjugated thermal responsive hybrid nanoparticles for photothermal and immunotherapy against cancer and its metastasis

Contact Info

Product

Resources

About