Size-related bacterial diversity and tetracycline resistance gene abundance in the air of concentrated poultry feeding operations

The Abundance and Diversity of Antibiotic Resistance Genes in the Atmospheric Environment of Biology Laboratories and Surroundings

“…The range of Staphylococcus. Spp in our samples was similar to its range in composting plants [25], while E.coli's abundance was close to concentrated poultry feeding operations [70], and higher than the composting plants [25].…”

“…The air from live poultry market had about 7.50 log(copies/m 3 ) tetG [24], which was even higher than the values in CL2 and CC1, 3.05×10 6 and 3.65×10 6 copies/m 3 , respectively ( Figure. 3 & Supplementary Table. Both tetW and sulII were widely detected in previous studies. The absolute abundances of tetW in our samples were higher than the ones in districts including railway stations areas, educational districts, medical districts, residential areas, and commercial districts [36,68], composting plants [25], clinics [62], and concentrated swine feeding operation [62], but comparable to concentrated poultry feeding operations [70]. Its relative abundances in our samples were between 0.027 to 0.27 copies/m 3 /16S rRNA copies/m 3 (Figure. 4 & Supplementary Table. 5 & 6, Additional File.1), similar to the ones in Nanjing [36].…”

Fungi diversity in PM<sub>2. 5</sub> and PM<sub>1</sub> at the summit of Mt. Tai: abundance, size distribution, and seasonal variation

“…This protocol can extract sufficient DNA from low-biomass environmental samples (e.g., aerosol particles) and boosted the DNA extraction efficiency by more than twice as compared to the non-optimized extraction method. Besides, it has been applied for studying airborne microbial diversity in different environments (Cao et al, 2014;Deng et al, 2016;Tong et al, 2017;Gao et al, 2017b). Half of the filters (about 121.64 cm 2 in area) were cut into small pieces, inserted into 50 mL Falcon tubes that were filled with sterilized 1× PBS buffer, and centrifuged at 200 × g for 3 h at 4 • C. The resuspension was collected into a 0.2 µm Supor 200 PES membrane disc filter.…”

acp-2017-204-RC2-Response to Reviewer #2

“…twice as compared to the non-optimized extraction method. Besides, it has been applied for studying airborne microbial diversity in different environments (Cao et al, 2014;Deng et al, 2016;Tong et al, 2017;Gao et al, 2017b). Half of the filters (about 121.64 cm 2 in area) were cut into small pieces, inserted into 50 mL Falcon tubes that were filled with sterilized 1× PBS buffer, and centrifuged at 200 × g for 3 h at 4 • C. The resuspension was collected into a 0.2 µm Supor 200 PES membrane disc filter.…”