Size‐related assessment on viability and insulin secretion of caprine islets in vitro

Abstract

The present findings evidenced the superior quality of smaller caprine islets compared to larger ones under an optimized basal maintenance condition. As it is equally important to preserve the quality of larger caprine islets, this work warrants further investigation on special culture conditions to support these islets.

Sero-epidemiology of Contagious Ecthyma Based on Detection of IgG Antibody in Selected Sheep and Goats Farms in Malaysia

“…Detail studies on adaptation of orf virus in tissue culture and embryonating eggs would pave the way for further studies to elucidate orf virus biology, mechanisms and preparation of the virus for vaccines to control and eradicate the disease Sadiq et al, 2017;Bala et al, 2018). It would be rather intriguing to determine the capability of orf virus to grow in cell lines of the original host perhaps to affect cell growth in vitroas well (Vakhshiteh et al, 2013;Hani et al, 2010). Besides parapoxviruses, herpesvirus family also portraying similar characteristics to grow in cell lines PCR and viral isolation were employed as confirmatory diagnosis for diseases.…”

Improvement of isolated caprine islet survival and functionality in vitro by enhancing of PDX1 gene expression

“…The insulin levels in all samples were determined using a commercial ovine insulin enzyme-linked immunosorbent assay (ELISA) kit (DRG, Germany). 34,35…”

Xenotransplantation literature update, March–April 2013

“…Vakhshiteh et al. examined the viability and insulin secretion of caprine islets in vitro . Caprine islet had a diameter between 50 and 250 μm, in which 80% of the total islet yield was defined as small islets with a diameter of ≤150 μm.…”