2012
DOI: 10.3109/08958378.2012.699984
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Size of submicrometric and nanometric particles affect cellular uptake and biological activity of macrophagesin vitro

Abstract: Particles were well characterized (fluorescence, size distribution, zeta potential, agglomeration and surface groups) and easily visualized after cellular uptake using confocal and electron microscopy. The number of internalized particles was precisely evaluated. Size was found to be an important parameter regarding particles uptake and in vitro toxicity but this latter strongly depends on the particles doses employed.

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Cited by 35 publications
(26 citation statements)
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“…Although further studies are clearly required to document the influence of Alhydrogel® agglomeration state on in vivo neurotoxic effects, such a finding would not be unprecedented in the field of particle toxicology since both cellular uptake and distribution in the body of other types of particles are influenced by the particle size (Buzea et al, 2007;Reddy et al, 2007;Landsiedel et al, 2012), and aggregation rate (Mühlfeld et al, 2008), two parameters that strongly determine particle toxicity (Bell et al, 2014;Leclerc et al, 2012;Nascarella and Calabrese, 2012;Mold et al, 2016). …”
Section: Discussionmentioning
confidence: 99%
“…Although further studies are clearly required to document the influence of Alhydrogel® agglomeration state on in vivo neurotoxic effects, such a finding would not be unprecedented in the field of particle toxicology since both cellular uptake and distribution in the body of other types of particles are influenced by the particle size (Buzea et al, 2007;Reddy et al, 2007;Landsiedel et al, 2012), and aggregation rate (Mühlfeld et al, 2008), two parameters that strongly determine particle toxicity (Bell et al, 2014;Leclerc et al, 2012;Nascarella and Calabrese, 2012;Mold et al, 2016). …”
Section: Discussionmentioning
confidence: 99%
“…Because TEM measurement requires samples to be air-dried before analysis, particle sizes measured by TEM are always slightly smaller than those from DLS with samples suspended in solution. 26 Generally speaking, the particle size directly measured from the TEM images was in good agreement with the DLS measurements. The release of siRNA from siRNA-NP and siRNA-NP-Ab (loaded with 1.5 nmol Cy3-labeled siRNA) was evaluated in PBS (pH 7.4) for 15 days ( Figure 2C).…”
Section: ■ Resultsmentioning
confidence: 86%
“…This previous work usually agrees with our data that smaller particles create an enhanced biological response. Typically, this previous work used engineered particles produced in laboratories at specific sizes, such as amorphous silica, titanium dioxide (TiO 2 ), or gold [18, 29, 35, 36]. Oberdorster et al observed significantly greater pulmonary inflammatory response to ultrafine (20-nm) TiO 2 in rats and mice when compared to larger particles (250-nm) [37].…”
Section: Discussionmentioning
confidence: 99%
“…Sager et al found that ultrafine (21-nm) TiO 2 particles caused significantly greater inflammation and were more cytotoxic than fine (1-μm) TiO 2 particles when instilled into rats [38]. Also, Leclerc et al investigated in vitro the rate of macrophage uptake and toxicity of fluorescent silica particles ranging from 850 to 150 nm and found that the smallest particles were internalized in greater quantities [18]. …”
Section: Discussionmentioning
confidence: 99%
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