Size of metallic and polyethylene debris particles in failed cemented total hip replacements

Lee, Joong-Myung; Salvati, Eduardo A.; Betts, F.; DiCarlo, Edward F.; Doty, Stephen B.; Bullough, Peter G.

doi:10.1302/0301-620x.74b3.1587882

Cited by 156 publications

(72 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our particles had an average size of 7 pm in diameter and we observed only small-to-moderate inflammatory changes, localized to the uppermost layers of the synovial membrane. Other studies indicate that smaller particles, with a relatively greater surface, are more pro-inflammatory (Lee et al 1992). Whether this also would increase the penetration and thereby the inflammatory response of PEP remains to be shown.…”

Section: Discussionmentioning

confidence: 93%

Polyethylene particles stimulate monocyte chemotactic and activating factor production in synovial mononuclear cells in vivo:An immunohistochemical study in rabbits

Frøkjær¹,

Lind²,

Overgaard³

et al. 1995

Acta Orthopaedica Scandinavica

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 93%

Polyethylene particles stimulate monocyte chemotactic and activating factor production in synovial mononuclear cells in vivo:An immunohistochemical study in rabbits

Frøkjær¹,

Lind²,

Overgaard³

et al. 1995

Acta Orthopaedica Scandinavica

View full text Add to dashboard Cite

“…In a rabbit model, Kubo et al [30] found that the material composition was more strongly correlated with histocyte reaction than with particle size and total surface area [21,22]. Lee et al [33] investigated the size of metallic and polyethylene particulate debris around failed arthroplasties. They have shown that metallic particles are submicron in mean size and polyethylene-particles are about 10-fold bigger.…”

Section: Discussionmentioning

confidence: 99%

Activation of NF-KB signalling and TNFα-expression in THP-1 macrophages by TiAlV- and polyethylene-wear particles

et al. 2005

View full text Add to dashboard Cite

Wear particles are believed to induce periprosthetic inflammation which contributes to periprosthetic osteolysis. TNFu plays a pivotal role in the pathogenesis of this process. The molecular mechanisms leading to the development of periprosthetic inflammation with upregulated TNFa expression in monocytic cells in response to different wear particles have yet to be defined. 1n:this study we evaluated the effects of polyethylene-and TiAIV-particles on activation of NF-KB signalling pathways and TNFa biosynthesis and release in monocytic cells with respect to periprosthetic osteoclastogenesis. THP-1 monocytic cells were differentiated to macrophage-like cells and exposed to LPS-detoxified polyethylene and prosthesis-derived TiAlV-particles. TNFa release was analyzed in culture supernatant by ELISA. NF-KB activation was examined by electrophoretic mobility shift assay (EMSA), and NF-KB target promoter activities including transactivation of the TNFa promoter were determined by luciferase reporter gene assays. Differentiated THP-1 macrophages were exposed to increasing numbers of particles for 0, 60, 180 and 360 min. Both, polyethylene-and TiAIV-particles induced a significant activation of both NF-KB and TNFa promoters at 180 min. A significant TNFu release was detected after 360 min exposure to polyethylene-and TiAlV-particles in a dose dependent manner. In comparison, LPS induced a much greater activation of NF-KB and TNFa promoters, and TNFa secretion into the supernatant was strongly induced. These results provide evidence that induction of the NF-KB signal transduction pathway in macrophages plays a major role in initiating and mediating the inflammatory response leading to periprosthetic osteolysis.

show abstract

“…The CoCr, TiAIV, and TIN particles were irregular to spherical in shape, and were in the same size range as metallic wear debris retrieved from tissues surrounding loosened implants [33,38,5 11. The silica particles were spherical and similar in morphology to particles implicated in reactive airway disease [39].…”

Section: Cliuructerixtion Oj'particlesmentioning

confidence: 91%

The role of adsorbed endotoxin in particle‐induced stimulation of cytokine release

Cho

Shanbhag

Hong

et al. 2002

Journal Orthopaedic Research

View full text Add to dashboard Cite

Numerous in vitro models have demonstraed the capacity of wear particles to stimulate the release of soluble pro‐inflammatory products with the ability to induce local bone resorption. Recent observations have demonstrated that binding of lipopolysaccharide (LPS) to particulate wear deris can significantly modulate the pattern of cell response in the in vitro models. These findings raise concerns over the possible role of LPS in the athogenesis of aseptic looseing after total joint replacements, and also indicates the importance of controlling for possible confounding effects of LPS contamination in the in vitro models used to study the reactive nature of wear debris. Our studies were undertaken to rigorously analyze the effects of particle‐associated LPS on cell responses and to assess the efficacy ofdifferent treatment protocols to inactivate LPS associated with different particulate materials. Particles of cobalt‐chrome alloy, titanium‐6‐aluminum‐4‐vanadium, titanium nitride and silica were pretreated with LPS and exposed to multiple treatment protocols. When cells were treated with „as‐received”︁ particles prepared by washing in ethanol, small amounts of TNF‐α, IL‐1β, and IL‐1α were detected. In contrast, all particle species pretreated with LPS produced marked increases in TNF‐α, IL‐1α, and IL‐1β release, as well as upregulation of corresponding mRNa levels even after ethanol washing. Boiling the LPS‐pretreated particles in 1% acetic acid or autoclaving and baking the particles also markedly reduced and in some instances abolished the effect of the LPS‐pretreatment. This indicates that LPS binds to the surface of particles of diverse composition and that the bound LPS is biologically active. Treatment protocols to inactivate particle‐associated LPS demonstrated significant differences in effcacy. When the most rigorous treatments were utilized, essentially all LPS activity could be eliminated. Particles treated with these methods retained some capacity to stimulated cytokine release, but activities were markedly reduced. These results provide further evidnce indicating that LPS contamination of particulate materials can markedly enhance their biological activity. This potential confounding effect needs to be carefully monitored and controlled in the in vitro model systems systems used to evaluate wear particles. Furthermore, the presence of particle‐associated endotoxin at the bone‐implant interface in vivo could markedly enhance the adverse biological activity of particulate wear debris. © 2002 Published by Elsevier Science Ltd. On behalf of Orthopaedic Research Society.

show abstract

Size of metallic and polyethylene debris particles in failed cemented total hip replacements

Cited by 156 publications

References 21 publications

Polyethylene particles stimulate monocyte chemotactic and activating factor production in synovial mononuclear cells in vivo:An immunohistochemical study in rabbits

Polyethylene particles stimulate monocyte chemotactic and activating factor production in synovial mononuclear cells in vivo:An immunohistochemical study in rabbits

Activation of NF-KB signalling and TNFα-expression in THP-1 macrophages by TiAlV- and polyethylene-wear particles

The role of adsorbed endotoxin in particle‐induced stimulation of cytokine release

Contact Info

Product

Resources

About