Size measurement of silica nanoparticles by Asymmetric Flow Field-Flow Fractionation coupled to Multi-Angle Light Scattering: A comparison exercise between two metrological institutes

Alasonati, Enrica; Caebergs, T.; Pétry, J.; Sebaïhi, Noham; Fisicaro, Paola; Feltin, Nicolas

doi:10.1016/j.chroma.2020.461859

Cited by 9 publications

(10 citation statements)

References 14 publications

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“…With the advent of nanotechnology, AF4 has evolved into an important characterization technique for nanoparticles, the traditional domain of dynamic light scattering (DLS) and single particle methods like nanoparticle tracking analysis or electron microscopy. Particle separation by flow FFF was pioneered in environmental research [13], and was further adapted for metal [14] and other nanoparticles [15], and latex particles [16]. At the beginning of the millennium AF4 was first used in pharmaceutical applications for proteins and particles for drug delivery [17], then followed by the work on liposomes [18], viruses and virus like particles [19], drug carriers [20], and extracellular vesicles and exosomes [21].…”

mentioning

confidence: 99%

Asymmetric Flow Field-Flow Fractionation: Current Status, Possibilities, Analytical Limitations and Future Trends

Podzimek

Johann²

2021

Chromatographia

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mentioning

confidence: 99%

Asymmetric Flow Field-Flow Fractionation: Current Status, Possibilities, Analytical Limitations and Future Trends

Podzimek

Johann²

2021

Chromatographia

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“…SEM analysis exhibited that the particle sizes of GOx-ALG-MCNPs formulation varied within a range of 53 to163 nm. The average particle size of the GOx-ALG-MCNPs was found to be 116.15 nm; during DLS analysis, the size is larger because of the measurement of hydrodynamic in this process [35].…”

Section: Morphology Particle Size Distribution and Zeta Potential Of Npsmentioning

confidence: 97%

Oral Glucose-Responsive Nanocarrier System for Management of Diabetes

et al. 2022

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Background:The regulation of postprandial blood glucose concentration in a diabetic person is a toilsome task by the conventional approaches. The continuous monitoring and subcutaneous injection of insulin are always imperative for diabetes management. To overcome the hurdle associated with the conventional system, a glucose-responsive nanoparticle (NP)-based approach was developed for oral insulin administration. The objective of the work is the formulation and characterization of a nanocarrier-based bio-responsive, self-regulated oral insulin drug delivery system. Methods:The mannose ligand-conjugated NPs were prepared by the inotropic gelation method in which insulin and glucose oxidase enclosed alginate nanocarrier cross-linked by tripolyphosphate. The prepared NPs were filled into the enteric-coated capsule and their release profile was determined at different pH and glucose concentrations. NPs were characterized by size distribution, % drug entrapment, enzyme activity, and in vivo and in vitro drug release studies. Results:The average size, surface potential, % drug entrapment of optimized NPs were 245.52 ± 3.37 nm, 22.12 ± 2.13, and 76.15±1.3%, respectively. The release of insulin from optimized NPs (GOx-ALG-MCNPs) (98.56±0.06%) occurs only at higher sugar concentrations (≥ 400 mg/dL) and prevents the release at low sugar concentrations (< 100 mg/dL). The enteric capsule shell protected the formulation to the gastric environment upon oral administration and directed them into the intestine. Mucoadhesion characteristics of NPs prolonged intestinal retention to make them available for cellular uptake. Glucose sensitivity and glucose-dependent effect on different pH environments study revealed that NPs released the drug only at higher concentrations of sugar solution while inhibiting the release at normal and lower sugar concentrations. Besides, compared to other prepared formulations, the GOx-ALG-MCNPs displayed controlled insulin release in response to glucose concentration.Conclusions: An important finding of GOx-ALG-MCNPs is autoregulating, glucose-responsive behavior imparting a significantly controlled lowering of blood glucose in an animal model. The findings reveal that the developed glucose-responsive NPs may prove to be a potential strategy for oral insulin delivery.

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“…The SEM image (figure 10(A)) shows smooth and oval-shaped particles, with a bell-shaped size distribution curve (figure 10(B)) ranging from 53 to 163 nm, with a mean frequency curve value of 116.15 nm. However, the DLS analysis (figure 10(C)) indicates larger sizes due to its hydrodynamic radius measurement principle [41]. The effects of various concentrations of CNPs and MNPs on RAW 264.7 cell inhibition were investigated through the MTT assay (figure 11).…”

Section: Characterization Of Mnpsmentioning

confidence: 99%

Quality by design (QbD) approach-based development of optimized nanocarrier to achieve quality target product profile (QTPP)-targeted lymphatic delivery

Maurya,

Ramteke,

Jain

2024

Nanotechnology

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Background: Insulin, vital for diabetes management, faces challenges with poor permeability and stability, demanding enhanced effectiveness and safety measures. Various oral peptide delivery systems have emerged, but non-targeted ones, while preventing degradation, lack the ability to facilitate peptide transport. The limitation lies in their non-selectivity. Intestinal macrophage cells in gut-associated lymphoid tissue express carbohydrate-binding receptors (CBRs), ideal for receptor-mediated endocytosis and peptide delivery.Objective: This study aimed to create mannose ligand conjugated nanoparticles (MNPs) using a quality-by-design (QbD) approach to tackle oral administration challenges. The secondary objective was to identify factors influencing product quality. Lymphatic uptake of NPs was the quality target product profile (QTPP), and the study focused on critical formulation attributes (CFAs) like Mannosylated Chitosan concentrations (MCs) and TPP concentrations, as well as critical process parameters (CPP) such as stirring speed.Methods: MNPs, produced via inotropic gelation, were encapsulated in enteric-coated capsules for protection. The impact of CFAs and CPP on particle size and entrapment was observed through Box-Behnken design (BBD), statistically analyzed with ANOVA (p<0.05). Optimal conditions were determined using desirability values. Characterization involved HPLC, zeta-seizer analysis, SEM for morphology, MTT assay for safety, and cell-line studies for M-cell targeting.Results: SEM revealed oval-shaped NPs with an average size of 245.52±3.37 nm, surface potential of 22.12±2.13, and 76.15±1.3% drug entrapment. MTT assay affirmed safety, while confocal imaging indicated selective M-cell uptake.Conclusion: BBD facilitated optimized NP formulation, providing clear insights into variable significance. Cell-line studies confirmed safety and effective cellular uptake of the NPs.

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Size measurement of silica nanoparticles by Asymmetric Flow Field-Flow Fractionation coupled to Multi-Angle Light Scattering: A comparison exercise between two metrological institutes

Cited by 9 publications

References 14 publications

Asymmetric Flow Field-Flow Fractionation: Current Status, Possibilities, Analytical Limitations and Future Trends

Asymmetric Flow Field-Flow Fractionation: Current Status, Possibilities, Analytical Limitations and Future Trends

Oral Glucose-Responsive Nanocarrier System for Management of Diabetes

Quality by design (QbD) approach-based development of optimized nanocarrier to achieve quality target product profile (QTPP)-targeted lymphatic delivery

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