Size-fractionation of natural phytoplankton communities in nutrient bioassay studies

Lane, Judith L.; Goldman, Charles R.

doi:10.1007/bf00021047

Cited by 24 publications

(14 citation statements)

References 15 publications

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“…The potential for trace metal limitation of phytoplankton growth in Crater Lake has been demonstrated by a small number of bioassay experiments (Groeger unpubl. data;Lane and Goldman 1984;Groeger and Teitjen 1993). However, identification of the limiting element and its effects on bacterioplankton growth require further investigation.…”

Section: Discussionmentioning

confidence: 99%

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Unusual bacterioplankton community structure in ultra‐oligotrophic Crater Lake

Urbach

Vergin

Young

et al. 2001

Limnology & Oceanography

210

243

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The bacterioplankton assemblage in Crater Lake, Oregon (U.S.A.), is different from communities found in other oxygenated lakes, as demonstrated by four small subunit ribosomal ribonucleic acid (SSU rRNA) gene clone libraries and oligonucleotide probe hybridization to RNA from lake water. Populations in the euphotic zone of this deep (589 m), oligotrophic caldera lake are dominated by two phylogenetic clusters of currently uncultivated bacteria: CL120-10, a newly identified cluster in the verrucomicrobiales, and ACK4 actinomycetes, known as a minor constituent of bacterioplankton in other lakes. Deep-water populations at 300 and 500 m are dominated by a different pair of uncultivated taxa: CL500-11, a novel cluster in the green nonsulfur bacteria, and group I marine crenarchaeota. ␤-Proteobacteria, dominant in most other freshwater environments, are relatively rare in Crater Lake (Յ16% of nonchloroplast bacterial rRNA at all depths). Other taxa identified in Crater Lake libraries include a newly identified candidate bacterial division, ABY1, and a newly identified subcluster, CL0-1, within candidate division OP10. Probe analyses confirmed vertical stratification of several microbial groups, similar to patterns observed in open-ocean systems. Additional similarities between Crater Lake and ocean microbial populations include aphotic zone dominance of group I marine crenarchaeota and green nonsulfur bacteria. Comparison of Crater Lake to other lakes studied by rRNA methods suggests that selective factors structuring Crater Lake bacterioplankton populations may include low concentrations of available trace metals and dissolved organic matter, chemistry of infiltrating hydrothermal waters, and irradiation by high levels of ultraviolet light.

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Section: Discussionmentioning

confidence: 99%

“…Depletion of nitrogen compounds near the surface suggests that phytoplankton growth is N-limited, though bioassay studies imply colimitation with a trace metal (Groeger unpubl. data;Lane and Goldman 1984;Groeger and Teitjen 1993). The lake receives no anthropogenic nutrient inputs aside from low amounts in precipitation and waste from seasonal tour boats.…”

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confidence: 99%

Unusual bacterioplankton community structure in ultra‐oligotrophic Crater Lake

Urbach

Vergin

Young

et al. 2001

Limnology & Oceanography

210

243

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“…Light-vs.-dark uptake of ['4C]glutamatc and [JH]thymidine in the bacterial layer was size-fractioned by sequential filtration (Lane and Goldman 1984). After filtration of thymidine samples, ice-cold 5% TCA was added to the filter towers.…”

Section: Methodsmentioning

confidence: 99%

Big Soda Lake (Nevada). 1. Pelagic bacterial heterotrophy and biomass1

Zehr

Harvey

Oremland

et al. 1987

Limnology & Oceanography

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Bacterial activities and abundance were measured seasonally in the water column of meromictic Big Soda Lake which is divided into three chemically distinct zones: aerobic mixolimnion, anaerobic mixolimnion, and anaerobic monimolimnion. Bacterial abundance ranged between 5 and 52 x 10h cells ml-', with highest biomass at the interfaces between these zones: 2-4 mg C liter' in the photosynthetic bacterial layer (oxycline) and 0.8-2.0 mg C liter' in the chemocline. Bacterial cell size and morphology also varied with depth: small coccoid cells were dominant in the aerobic mixolimnion, whereas the monimolimnion had a more diverse population that included cocci, rods, and large filaments. Heterotrophic activity was measured by [methyl-3H]thymidine incorporation and [14C]glutamate uptake. Highest uptake rates were at or just below the photosynthetic bacterial layer and were attributable to small (< 1 pm) heterotrophs rather than the larger photosynthetic bacteria. These high rates of heterotrophic uptake were apparently linked with fermentation; rates of other mineralization processes (e.g. sulfate reduction, methanogenesis, denitrification) in the anoxic mixolimnion were insignificant. Heterotrophic activity in the highly reduced monimolimnion was generally much lower than elsewhere in the water column. Therefore, although the monimolimnion contained most of the bacterial abundance and biomass (-60%), most of the cells there were inactive.

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“…At all depths, dissolved N/P molar ratios fall below the Redfield ratio of 16, suggesting that autotrophic growth may be N-limited (Redfield, 1958). Bioassay studies also suggest that phytoplankton productivity may be co-limited by N and trace metals, with different size fractions showing differences in growth stimulation by N and metal mixtures (Lane & Goldman, 1984;Groeger & Teitjen, 1993;Groeger, 2007). Crater lake shares a number of features with oligotrophic, open ocean waters, including oxygenated deep waters, the possibility of combined N and trace metal limitation, a deep chlorophyll maximum, and exceptionally high light transmittance (Urbach et al, 2001).…”

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confidence: 96%

Bacterioplankton communities of Crater Lake, OR: dynamic changes with euphotic zone food web structure and stable deep water populations

Urbach

Vergin

Larson³

et al. 2007

Hydrobiologia

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The distribution of bacterial and archaeal species in Crater Lake plankton varies dramatically over depth and with time, as assessed by hybridization of group-specific oligonucleotides to RNA extracted from lakewater. Nonmetric, multidimensional scaling (MDS) analysis of relative bacterial phylotype densities revealed complex relationships among assemblages sampled from depth profiles in July, August and September of 1997 through 1999. CL500-11 green nonsulfur bacteria (Phylum Chloroflexi) and marine Group I crenarchaeota are consistently dominant groups in the oxygenated deep waters at 300 and 500 m. Other phylotypes found in the deep waters are similar to surface and middepth populations and vary with time. Euphotic zone assemblages are dominated either by bproteobacteria or CL120-10 verrucomicrobia, and ACK4 actinomycetes. MDS analyses of euphotic zone populations in relation to environmental variables and phytoplankton and zooplankton population structures reveal apparent links between Daphnia pulicaria zooplankton population densities and microbial community structure. These patterns may reflect food web interactions that link kokanee salmon population densities to community structure of the bacterioplankton, via fish predation on Daphnia with cascading consequences to Daphnia bacterivory and predation on bacterivorous protists. These results demonstrate a stable bottom-water microbial community. They also extend previous observations of food web-driven changes in euphotic zone bacterioplankton community structure to an oligotrophic setting.

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Size-fractionation of natural phytoplankton communities in nutrient bioassay studies

Cited by 24 publications

References 15 publications

Unusual bacterioplankton community structure in ultra‐oligotrophic Crater Lake

Unusual bacterioplankton community structure in ultra‐oligotrophic Crater Lake

Big Soda Lake (Nevada). 1. Pelagic bacterial heterotrophy and biomass1

Bacterioplankton communities of Crater Lake, OR: dynamic changes with euphotic zone food web structure and stable deep water populations

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