Size Exclusion of Radioactive Polymers (SERP) informs on the biodegradation of trimethyl chitosan and biodegradable polymer nanoparticles in vitro and in vivo

Dikpati, Amrita; Gaudreault, Nicolas; Chénard, Valérie; Grenier, Pierre; Boisselier, Élodie; Bertrand, Nicolas

doi:10.1016/j.jconrel.2022.04.009

Cited by 9 publications

(6 citation statements)

References 63 publications

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“…In these conditions, formulations containing BD650 and Cy5 released fluorescent and radioactive labels at similar speeds and the ratios of each signal remained constant over a period of 72 h. In contrast, nanoparticles labelled with AZ647 showed a rapid release of their fluorescent signal: the ratio of fluorescence to radioactivity had decreased by 25% within 6 h, and by as much as 75% after 2 days. We have recently developed a method called size exclusion of radioactive polymers (SERP) that allows the detection of radioactive degradation products released from nanoparticles [ 33 ]. Simply put, this method uses Sephadex exclusion columns to separate large nanoparticles from smaller hydrolysis products.…”

Section: Resultsmentioning

confidence: 99%

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Dual-Labelled Nanoparticles Inform on the Stability of Fluorescent Labels In Vivo

et al. 2023

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Fluorescent labelling is commonly used to monitor the biodistribution of nanomedicines. However, meaningful interpretation of the results requires that the fluorescent label remains attached to the nanomedicine. In this work, we explore the stability of three fluorophores (BODIPY650, Cyanine 5 and AZ647) attached to polymeric hydrophobic biodegradable anchors. Using dual-labelled poly(ethylene glycol)-b-poly(lactic acid) (PEG-PLA) nanoparticles that are both radioactive and fluorescent, we investigated how the properties of the fluorophores impact the stability of the labelling in vitro and in vivo. Results suggest that the more hydrophilic dye (AZ647) is released faster from nanoparticles, and that this instability results in misinterpretation of in vivo data. While hydrophobic dyes are likely more suitable to track nanoparticles in biological environments, quenching of the fluorescence inside the nanoparticles can also introduce artefacts. Altogether, this work raises awareness about the importance of stable labelling methods when investigating the biological fate of nanomedicines.

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Section: Resultsmentioning

confidence: 99%

“…The size exclusion chromatography was adapted from a method already described elsewhere [ 33 ]. Briefly, 200 µL of a 5 mg/mL solution of dual-labelled nanoparticles was loaded on a Sephadex G-75 (1 × 20 cm, DWK Life Sciences Kimble TM FlexColumn, #4204011020, Vineland, NJ, USA) in isotonic phosphate-buffered saline solution pH = 7.2.…”

Section: Methodsmentioning

confidence: 99%

Dual-Labelled Nanoparticles Inform on the Stability of Fluorescent Labels In Vivo

et al. 2023

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“…In previous research, evaluating and tracking the degradation of implanted biodegradable polymers often relied on labeling the polymers with carbon-14 isotopes (C-14) 67 , 68 , 69 . However, this approach has several drawbacks.…”

Section: In Vivo Evaluation Of Resorbabilitymentioning

confidence: 99%

“…This absorption allows for enhanced signal-tonoise ratio and specificity when visualizing the PDLLA implant using MIR-PAM. In previous research, evaluating and tracking the degradation of implanted biodegradable polymers often relied on labeling the polymers with carbon-14 isotopes (C-14) 67 , 68 , 69 . However, this approach has several drawbacks.…”

Section: In Vivo Evaluation Of Resorbabilitymentioning

confidence: 99%

In vivo evaluation of thermally drawn biodegradable optical fibers as brain neural interfaces

Abdollahian,

Sui,

et al. 2024

Preprint

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Optical fiber technology has a critical role in modern neuroscience towards understanding the complex neuronal dynamics within the nervous system. In this study, we manufactured amorphous thermally drawn poly D, L-lactic acid (PDLLA) optical fibers in different diameters. These fibers were then implanted into the lateral posterior region of the mouse brain for 4 months, allowing us to assess their degradation characteristics. The gradual dissolution of the implanted PDLLA optical fibers in the brain was confirmed by optical microscope and scanning electron microscopy (SEM), molecular weight measurements, and light transmission spectroscopy. The results indicate that the degradation rate was mainly pronounced during the first week. Degradation after 4 months resulted in the formation of micropores on the surface of the implanted fiber within the gray matter region of the brain. Moreover, the current PDLLA optical fiber implant offers efficient light transmission in the short-wavelength near-infrared (SW-NIR) range. SW-NIR enables deeper tissue penetration and reduced light scattering, making it ideal for optogenetics and functional imaging with therapeutic potential in neurological disorders. We believe that the provided PDLLA optical fiber in this study constitutes a promising candidate for the development of next-generation biocompatible, soft, and biodegradable bi-directional neural interfaces.

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“…Chitosan (CS), a cationic biopolymer derived from chitin, exhibits favorable characteristics for delivering nucleic acids, including electrostatic interaction with nucleic acids, biocompatibility, and susceptibility to chemical modifications. The transfection efficiency of chitosan-based systems has been demonstrated to be influenced by the precise interplay of various CS parameters, such as the charge density or degree of deacetylation (DDA), molecular weight (Mn), the ratio of amine groups to phosphate groups (N:P), and environmental factors like pH and serum proteins [ 145 , 146 , 147 , 148 , 149 ]. As demonstrated in Table 3 , this naturally occurring linear copolymer consists of 2-amino-2-deoxy-D-glucopyranose and 2-acetamido-2-deoxy-D-glucopyranose.…”

Section: Nonviral Delivery Systemsmentioning

confidence: 99%

Emerging Perspectives on Prime Editor Delivery to the Brain

BenDavid,

Ramezanian,

et al. 2024

Pharmaceuticals

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Prime editing shows potential as a precision genome editing technology, as well as the potential to advance the development of next-generation nanomedicine for addressing neurological disorders. However, turning in prime editors (PEs), which are macromolecular complexes composed of CRISPR/Cas9 nickase fused with a reverse transcriptase and a prime editing guide RNA (pegRNA), to the brain remains a considerable challenge due to physiological obstacles, including the blood–brain barrier (BBB). This review article offers an up-to-date overview and perspective on the latest technologies and strategies for the precision delivery of PEs to the brain and passage through blood barriers. Furthermore, it delves into the scientific significance and possible therapeutic applications of prime editing in conditions related to neurological diseases. It is targeted at clinicians and clinical researchers working on advancing precision nanomedicine for neuropathologies.

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Size Exclusion of Radioactive Polymers (SERP) informs on the biodegradation of trimethyl chitosan and biodegradable polymer nanoparticles in vitro and in vivo

Cited by 9 publications

References 63 publications

Dual-Labelled Nanoparticles Inform on the Stability of Fluorescent Labels In Vivo

Dual-Labelled Nanoparticles Inform on the Stability of Fluorescent Labels In Vivo

In vivo evaluation of thermally drawn biodegradable optical fibers as brain neural interfaces

Emerging Perspectives on Prime Editor Delivery to the Brain

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