Size-exclusion HPLC provides a simple, rapid, and versatile alternative method for quality control of vaccines by characterizing the assembly of antigens

Yang, Yanli; Li, Hao; Li, Zhengjun; Zhang, Yan; Zhang, Songping; Chen, Yi; Yu, Mengran; Ma, Guanghui; Su, Zhiguo

doi:10.1016/j.vaccine.2015.01.031

Cited by 63 publications

(53 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…is a chromatographic method which separated the molecules by the size, widely applied in larger molecules purification, very suitable for virus or VLP [19]. However, molecular sieving can bring mechanical injury to some kinds of viruses.…”

Section: Discussionmentioning

confidence: 99%

A common problem in the purification of calicivirus raised by molecular sieving: balance between purification and loss of viral particles

Kang

Meng

et al. 2020

Preprint

View full text Add to dashboard Cite

Caliciviridae is a group of RNA viruses could lead to gastrointestinal disease in humans and acute oral or upper respiratory tract disease in felines, no efficient vaccine so far. The preparation of immunogen is a challenge to obtain a safe and efficient vaccine candidate.Molecular sieving, due to the advantage of simple and fast purifying, is considered as a very powerful tool in calicivirus purification; however, also introduce mechanical damage to the viron. In this study, we took Feline Calicivirus (FCV), a common natural pathogen in cats as well as a classic model for caliciviruses studies, as a research object. Here, with the help of the cryo-electron microscope (cryo-EM), we collected particle images in both conditions of with and without molecular sieving post-processing, the impact of molecular sieving on FCV particles was evaluated by building a flexibility evaluation system after 3D classification. The results indicated that the molecular sieving will impact the stability of P domains through increasing flexibility. In addition, we successfully built up a scoring system to describe this flexibility of FCV P-domains, which can be extended to assess the purification effect to the entire calicivirus.

show abstract

Section: Discussionmentioning

confidence: 99%

A common problem in the purification of calicivirus raised by molecular sieving: balance between purification and loss of viral particles

Kang

Meng

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…For both emulsions, the final content of OVA and FMDV in the emulsion system was about 1 mg/ml and 10 lg/ml, respectively. The FMDV antigen concentration was determined by size-exclusion HPLC method, which was developed in our lab (Yang et al 2015).…”

Section: Emulsion Preparationmentioning

confidence: 99%

“…FMDV antigen content in the aqueous phase recovered from the emulsion was quantitatively determined by size-exclusion HPLC method developed in our lab (Yang et al 2015). The antigen content in aqueous phase before being emulsified with oil phase was also determined for comparison.…”

Section: Effect Of Emulsification On Fmdv Antigenmentioning

confidence: 99%

“…During preparation of the nano-emulsion, there was a high shear force that may cause dissociation of the 146S. Therefore, the 146S content in aquous phase before and after emulsification was determined with size exculsion HPLC analysis according to protocol developed in our lab (Yang et al 2015). The HPLC chromtogaphs shown in Figure 11 indicated that the FMDV recoverd from nano-emulsion was eluted at the same time as that of before emulsification, indicating there was no occurrence of aggragation or dissociation in 146S during emulsification, thus its immunogenicity would be affected.…”

Section: Stability Of Nano-emulsion With Antigens and Effect Of Emulsmentioning

confidence: 99%

See 1 more Smart Citation

Construction of a stable w/o nano-emulsion as a potential adjuvant for foot and mouth disease virus vaccine

Chen

Zhang

et al. 2016

Artificial Cells, Nanomedicine, and Biotechnology

Self Cite

View full text Add to dashboard Cite

A stable and bio-compatible w/o nano-emulsion was developed as adjuvant for foot and mouth disease (FMD) vaccine with isopropyl myristate as oil phase, polyglycerol polyricinoleate, and Tween 80 as surfactants. Dissolving Tween 80 in aqueous phase decreased the droplet size and improved stability of the emulsion through narrowing size distribution, increasing viscosity, and reducing the interfacial tension between oil and water. The nano-emulsion was then formulated with inactivated FMD virus and ovalbumin to investigate its potential application as an adjuvant. Compared with commercial ISA-206 adjuvant, the novel w/o nano-emulsion was able to induce both humoral- and cell-mediated immune response.

show abstract

“…Size exclusion-high performance liquid chromatography (SE-HPLC) is a chromatographic method in which molecules in solution is separated by their size, and in some cases molecular weight. It has been widely used for the separation of large molecules or macromolecular complexes such as proteins and industrial polymers (YANG et al, 2015). In wheat, SE-HPLC has been successfully used to separate and characterize seed proteins, including water-soluble proteins (BEAN et al, 2003;WANG et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Effects of glu-1 and glu-3 allelic variations on wheat glutenin macropolymer (GMP) content as revealed by size-exclusion high performance liquid chromatography (SE-HPLC)

et al. 2017

Genetika

View full text Add to dashboard Cite

2017): Effects of glu-1 and glu-3 allelic variations on wheat glutenin macropolymer (GMP) content as revealed by size-exclusion high performance liquid chromatography vol 49, no. 2,(677)(678)(679)(680)(681)(682)(683)(684)(685)(686)(687)(688)(689)(690)(691) Gluten macropolymers (GMPs), formed by seed storage proteins glutenins and gliadins through intermolecular disulfide bond, confer dough viscoelasticity and wheat processing quality. Glutenins consist of high and low molecular weight glutenin subunits (HMW-GS, LMW-GS) encoded by Glu-1 and Glu-3 loci, respectively. The allelic variations at both loci have important effects on GMP content and breadmaking quality. In this study, GMP extraction and size-exclusion high performance liquid chromatography (SE-HPLC) separation were optimized, and then applied to investigate the effects of Glu-1 and Glu-3 allelic variations on GMP content using different wheat genotypes, chromosome 678 GENETIKA, Vol. 49, No.2, 677-691, 2017 substitution lines and near-isogenic lines (NILs). The results showed that the optimized GMP extraction and SE-HPLC protocol could obtain a reproducible separation and reliable quantitation of GMP content with small samples. The allelic variations at Glu-1 and Glu-3 were closely related to GMP content. Particularly, Glu-D1d encoded 1Dx5+1Dy10 subunits, Glu-A3a and Glu-B3h encoded an abundant LMW-B subunit respectively had positive effects on GMP content and breadmaking quality. The introgress of HMW-GS and LMW-GS into bread wheat from related genomes could significantly increase GMP content, indicating that wheat related species has potential gene resources for breadmaking quality improvement. Our results demonstrated that SE-HPLC could serve as an effective tool for rapid separation and quantitation of GMPs and had potential application for gluten quality screening in the early generations during wheat quality improvement program.

show abstract

Size-exclusion HPLC provides a simple, rapid, and versatile alternative method for quality control of vaccines by characterizing the assembly of antigens

Cited by 63 publications

References 25 publications

A common problem in the purification of calicivirus raised by molecular sieving: balance between purification and loss of viral particles

A common problem in the purification of calicivirus raised by molecular sieving: balance between purification and loss of viral particles

Construction of a stable w/o nano-emulsion as a potential adjuvant for foot and mouth disease virus vaccine

Effects of glu-1 and glu-3 allelic variations on wheat glutenin macropolymer (GMP) content as revealed by size-exclusion high performance liquid chromatography (SE-HPLC)

Contact Info

Product

Resources

About