Size-exclusion chromatography of metal nanoparticles and quantum dots

Pitkänen, Leena; Striegel, André M.

doi:10.1016/j.trac.2015.06.013

Cited by 88 publications

(56 citation statements)

References 60 publications

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“…Typically used for the separation and analysis of macromolecules and viruses, size exclusion chromatography is increasingly used for the separation of polydisperse populations of nanoparticles according to their hydrodynamic radius . A SEC separation column is packed with porous microparticles featuring different pore apertures ranging from a few nanometers up to hundreds of nanometers, forming the so‐called solid phase of the column.…”

Section: Characterization Methodsmentioning

confidence: 99%

“…Particles with different surface charges and shape can interact differently with the solid phase, which complicates the prediction and translation of elution times into size information. However, the compatibility of this technique with industrial production and characterization is promoting efforts toward the investigation of SEC separation for quantitative nanoparticle size analysis …”

Section: Characterization Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Nanoparticle Characterization: What to Measure?

et al. 2019

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What to measure? is a key question in nanoscience, and it is not straightforward to address as different physicochemical properties define a nanoparticle sample. Most prominent among these properties are size, shape, surface charge, and porosity. Today researchers have an unprecedented variety of measurement techniques at their disposal to assign precise numerical values to those parameters. However, methods based on different physical principles probe different aspects, not only of the particles themselves, but also of their preparation history and their environment at the time of measurement. Understanding these connections can be of great value for interpreting characterization results and ultimately controlling the nanoparticle structure–function relationship. Here, the current techniques that enable the precise measurement of these fundamental nanoparticle properties are presented and their practical advantages and disadvantages are discussed. Some recommendations of how the physicochemical parameters of nanoparticles should be investigated and how to fully characterize these properties in different environments according to the intended nanoparticle use are proposed. The intention is to improve comparability of nanoparticle properties and performance to ensure the successful transfer of scientific knowledge to industrial real‐world applications.

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Section: Characterization Methodsmentioning

confidence: 99%

Section: Characterization Methodsmentioning

confidence: 99%

Nanoparticle Characterization: What to Measure?

et al. 2019

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“…Different methods are used for quantitative detection of nanoparticles in water which are mainly based on a combination of size separation techniques (e.g., size-exclusion chromatography [12], field flow fractionation [13,14], capillary electrophoresis [15]) with various detectors (e.g., UV, mass-and inductively coupled plasma mass spectrometry [16,17]. For the majority of these methods, a granulometric composition of the starting particles mixture is important, and the deviation from the true size distribution might give wrong results [11,18,19].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous spectrophotometric determination of titanium oxide and iron oxide nanoparticles in water by using PLS algorithm

et al. 2019

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The method for determination of the component partial concentrations in mixed two-component water suspension from the UV spectrophotometry by applying the PLS algorithm is suggested. This quantitative method does not require special probe preparations, it is convenient and inexpensive and it can be realized for water quality monitoring by using portable devices. As an example, the water suspensions of TiO 2 and Fe 3 O 4 -coated by SiO 2 nanoparticles were analyzed. The concentration range for the simultaneous determination of nanoparticles is 0.5-10 mg/l. The relative standard errors for single components determination are 1.8% for TiO 2 and 3.9% for Fe 3 O 4 -coated by SiO 2 . The processes of nanoparticles aggregation in the studied mixed suspensions are reversible and do not distort significantly the results of the partial concentrations determination. The method suggested can be used for detection of the residual partial concentrations of titanium oxides and iron-containing nanoparticles in water treatment technologies.

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“…Thus, the development of methods to separate colloidal mixtures of nanoparticles based on properties such as shape, size and also surface functionalization has been an important research area for several years. 2,[11][12][13] This has led to development of several techniques including density gradient centrifugation, 14,15 dialtration, 11 eld-ow fractionation, 16 and size-exclusion chromatography 17 as well as a number of electrophoretic methods. [18][19][20][21] Capillary electrophoresis (CE) has been successfully applied to the separation of a wide range of nanoparticles based on different migration times under an applied electric eld through a narrow glass capillary in the presence of background electrolytes.…”

Section: Introductionmentioning

confidence: 99%

Gel electrophoretic analysis of differently shaped interacting and non-interacting bioconjugated nanoparticles

2016

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The use of a simple gel electrophoretic method to study mixtures of differently shaped biofunctionalized nanoparticles (NP's) that undergo bioaffinity interactions is demonstrated. Both gold nanorods (NR's) and quasi-spherical nanoparticles (qNS's) were functionalized with an interacting antigen and antibody pairing (alpha-1 antitrypsin (AAT) protein and antiAAT) or non-interacting antibody controls (antiBNP). Gel-based measurements were accompanied with transmission electron microscopy (TEM) and UV-vis spectroscopy analysis before and after separation. Initial measurements of NR and qNS bioconjugates suspended individually were applied to optimize the gel separation conditions and it was demonstrated that higher particle uniformities could be obtained relative to the initial stock solutions. A series of NR and qNS mixtures prepared at various stoichiometric ratios were then compared for both interacting (antiAAT-AAT) and non-interacting (antiAAT-antiBNP) particle conjugates. Both gel images and extinction measurements were utilized to demonstrate reduced NP concentrations transported along the gel due to bioaffinity-induced NP assembly. This confirmed that gel electrophoresis can be extended to identifying particle aggregation associated with protein bioaffinity interactions as well as being an established tool for separating particles based on size, shape and surface chemistry.

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Size-exclusion chromatography of metal nanoparticles and quantum dots

Cited by 88 publications

References 60 publications

Nanoparticle Characterization: What to Measure?

Nanoparticle Characterization: What to Measure?

Simultaneous spectrophotometric determination of titanium oxide and iron oxide nanoparticles in water by using PLS algorithm

Gel electrophoretic analysis of differently shaped interacting and non-interacting bioconjugated nanoparticles

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