Size-exclusion chromatography-based extracellular vesicle size subtyping and multiplex membrane protein profiling for differentiating gastrointestinal cancer prognosis

Qin, Ti; Li, Pinhao; Li, Jun; Guo, Qianqian; Chen, Ying; Wang, Yu-E.; Tao, Ling; Huang, Jian; Shen, Xiangchun; Wu, Xingjie

doi:10.1039/d3an01027a

Cited by 2 publications

(1 citation statement)

References 37 publications

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“…In this study, we separated EVs from clinical samples using size exclusion chromatography (SEC), which is based on molecular size. When the sample flows through the porous stationary phase, the smaller proteins and other impurities in the sample enter the pore and are not easily washed out, while the EVs with larger particle size in the sample do not enter the pore and flow out more quickly, thus achieving the separation of EVs [ 38 , 39 ]. Compared with the ultracentrifugation, it harbors higher efficiency.…”

Section: Discussionmentioning

confidence: 99%

Urinary extracellular vesicle-derived miR-126-3p predicts lymph node invasion in patients with high-risk prostate cancer

Dong,

Hu,

et al. 2024

Med Oncol

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To investigate extracellular vesicles (EVs), biomarkers for predicting lymph node invasion (LNI) in patients with high-risk prostate cancer (HRPCa), plasma, and/or urine samples were prospectively collected from 45 patients with prostate cancer (PCa) and five with benign prostatic hyperplasia (BPH). Small RNA sequencing was performed to identify miRNAs in the EVs. All patients with PCa underwent radical prostatectomy and extended pelvic lymph node dissection. Differentially expressed miRNAs were identified in patients with and without pathologically-verified LNI. The candidate miRNAs were validated in low-risk prostate cancer (LRPCa) and BPH. Four miRNA species (e.g., miR-126-3p) and three miRNA species (e.g., miR-27a-3p) were more abundant in urinary and plasma EVs, respectively, of patients with PCa. None of these miRNA species were shared between urinary and plasma EVs. miR-126-3p was significantly more abundant in patients with HR PCa with LNI than in those without (P = 0.018). miR-126-3p was significantly more abundant in the urinary EVs of patients with HRPCa than in those with LRPCa (P = 0.017) and BPH (P = 0.011). In conclusion, urinary EVs-derived miR-126-3p may serve as a good biomarker for predicting LNI in patients with HRPCa.

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Section: Discussionmentioning

confidence: 99%

Urinary extracellular vesicle-derived miR-126-3p predicts lymph node invasion in patients with high-risk prostate cancer

Dong,

Hu,

et al. 2024

Med Oncol

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Aptamer biorecognition and gold nanoshell-mediated fluorescence quenching for glycoprotein analysis and breast cancer diagnosis

Wu,

Liu,

Chen

et al. 2024

Microchemical Journal

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Size-exclusion chromatography-based extracellular vesicle size subtyping and multiplex membrane protein profiling for differentiating gastrointestinal cancer prognosis

Abstract: Extracellular vesicles (EVs), as a type of subcellular structure, have been extensively researched for their potential in developing advanced diagnostic technologies for various diseases. However, the biomolecular and biophysical heterogeneity...

Cited by 2 publications

References 37 publications

Urinary extracellular vesicle-derived miR-126-3p predicts lymph node invasion in patients with high-risk prostate cancer

Urinary extracellular vesicle-derived miR-126-3p predicts lymph node invasion in patients with high-risk prostate cancer

Aptamer biorecognition and gold nanoshell-mediated fluorescence quenching for glycoprotein analysis and breast cancer diagnosis

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