Size-Exclusion Chromatography

Striegel, André M.

doi:10.1016/b978-0-12-415807-8.00009-2

Cited by 18 publications

(11 citation statements)

References 47 publications

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“…The peaks were observed at elusion times 60 min, 130 min and 245 min, which refer to high, intermediate and low molecular weight bands, respectively. According to Brookes et al [91] and Striegel [92], the retention time of the molecules in the chromatography column is inversely proportional to their molecular weights. Hence, the largest molecules are eluted at the lowest elution times, which is called an "inverse-sieving" mechanism.…”

Section: Chromatographic Separation Of Prepolymerized Aluminium Coagumentioning

confidence: 99%

Strategy for Flux Enhancement in Biofilm Ceramic Membrane Bioreactor Applying Prepolymerized and Non-Prepolymerized Inorganic Coagulants

Kulesha

Maletskyi

Kvaal

et al. 2019

Water

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Considering new legislative and economic restrictions caused by the water crisis, this work focuses on a more efficient wastewater treatment process, which combines biological treatment in a moving bed biofilm system with a membrane bioreactor (BF-MBR) and coagulation, particularly addressing fouling alleviation in the separation stage. The study justifies the positive impact of coagulant dosing in BF-MBR regarding membrane flux and fouling rate. Statistical techniques connect the results of coagulation and membrane separation experiments with properties of mixed liquor, obtained after biotreatment in the representative pilot plant and characteristics of prepolymerized and non-prepolymerized inorganic coagulants. Research results substantiate the need for a pH-controlled coagulation of mixed liquor in BF-MBR depending on coagulant type, which influences charge, hydrophobicity and size of flocs and organic content of the system. It is suggested, that the adsorption/charge neutralization mechanism dominates in flux enhancement in BF-MBR, giving the best results in the case of prepolymerized aluminium coagulants. Together with high quality of permeate, the application of prepolymerized aluminium chloride of medium basicity entails a tenfold increase in filtration time of the membrane separation cycle and increases net membrane flux by 30–56%. The results of the study are practically significant for the development of an automated control system for BF-MBR, optimizing treatment rates together with membrane separation efficiency.

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Section: Chromatographic Separation Of Prepolymerized Aluminium Coagumentioning

confidence: 99%

Strategy for Flux Enhancement in Biofilm Ceramic Membrane Bioreactor Applying Prepolymerized and Non-Prepolymerized Inorganic Coagulants

Kulesha

Maletskyi

Kvaal

et al. 2019

Water

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“…This phenom enon enables the separation of a broad range of sizes, but results in poor selectivity 88 . In size exclusion chromatography (SEC) columns, packed with porous material, size fractionation results from the fast est elution of bigger particles and delayed elution of particles of size smaller than or equal to that of the pores 89 . The resolving power of chromatographic separation can be compromised by the interaction of nanoparticles with the stationary phase or with the species present in the mobile phase.…”

Section: Detection and Quantification Of Enps At Low Concentrationsmentioning

confidence: 99%

Bridging the divide between human and environmental nanotoxicology

2015

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The need to assess the human and environmental risks of nanoscale materials has prompted the development of new metrological tools for their detection, quantification and characterization. Some of these methods have tremendous potential for use in various scenarios of nanotoxicology. However, in some cases, the limited dialogue between environmental scientists and human toxicologists has hampered the full exploitation of these resources. Here we review recent progress in the development of methods for nanomaterial analysis and discuss the use of these methods in environmental and human toxicology. We highlight the opportunities for collaboration between these two research areas.

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“…This is essential in the field of venomics, as toxins are often large proteins that may or may not exist in complexes with other proteins [48,49]. This technique can also be achieved using HPLC to perform a high-resolution fractionation of molecules based on their molecular weight [47]. However, depending on the separation technology used to achieve SEC, it is not a particularly high-resolution chromatographic technique and has a limited dynamic range for protein separation.…”

Section: Separation Techniquesmentioning

confidence: 99%

“…SEC is a chromatographic method in which hydrophilic molecules (proteins or other solutes) are separated based on their size or molecular weight, either through molecular-weight sieves or through gel-filtration chromatography [ 47 ]. It has proven to be very useful in separating proteins, due to the technique’s ability to separate native proteins (undigested) or protein complexes.…”

Section: Techniques In Venomicsmentioning

confidence: 99%

“…However, depending on the separation technology used to achieve SEC, it is not a particularly high-resolution chromatographic technique and has a limited dynamic range for protein separation. For example, the use of molecular-weight sieves, while very user-friendly, has a far lower chromatographic resolution than HPLC would [ 47 ].…”

Section: Techniques In Venomicsmentioning

confidence: 99%

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Proteomic and Transcriptomic Techniques to Decipher the Molecular Evolution of Venoms

Mouchbahani-Constance

Sharif‐Naeini

2021

Toxins

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Nature’s library of venoms is a vast and untapped resource that has the potential of becoming the source of a wide variety of new drugs and therapeutics. The discovery of these valuable molecules, hidden in diverse collections of different venoms, requires highly specific genetic and proteomic sequencing techniques. These have been used to sequence a variety of venom glands from species ranging from snakes to scorpions, and some marine species. In addition to identifying toxin sequences, these techniques have paved the way for identifying various novel evolutionary links between species that were previously thought to be unrelated. Furthermore, proteomics-based techniques have allowed researchers to discover how specific toxins have evolved within related species, and in the context of environmental pressures. These techniques allow groups to discover novel proteins, identify mutations of interest, and discover new ways to modify toxins for biomimetic purposes and for the development of new therapeutics.

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Size-Exclusion Chromatography

Cited by 18 publications

References 47 publications

Strategy for Flux Enhancement in Biofilm Ceramic Membrane Bioreactor Applying Prepolymerized and Non-Prepolymerized Inorganic Coagulants

Strategy for Flux Enhancement in Biofilm Ceramic Membrane Bioreactor Applying Prepolymerized and Non-Prepolymerized Inorganic Coagulants

Bridging the divide between human and environmental nanotoxicology

Proteomic and Transcriptomic Techniques to Decipher the Molecular Evolution of Venoms

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