Size Distribution of Lymph Cells

Abstract

With the presently available equipment (Coulter counter in combination with a multichannel analyzer) reproducible and biologically meaningfull cell volume distributions can be recorded within a short time (2–3 min) from a large sample of cells (105). Lymph cells remain nearly stable for 2–3 hours in lymph with heparin as anticoagulant. On dilution with most of the presently available ‘isotonic’ and ‘balanced’ salt solutions the lymph cells immediately start to swell or shrink. In two diluents a labile equilibrium of volume distribution is maintained for about 10 min. During this time a reproducible size distribution diagram can be recorded. Some potentialities and limitations of the method are discussed on the basis of two experiments: the changes in size distribution of cells in the efferent lymph of an antigenically stimulated lymph node and in thoracic duct lymph in the course of a depletion of the pool of recirculating lymphocytes.

Eine Methode zur Langzeit-Sammlung der efferenten Lymphe des Buglymphknotens beim Schaf1

“…Die Vielkanalanalysator bei quantitativer Lymph-Sammlung. Diese Methodik wird andernorts beschrieben werden (5). Zur Auswertung der Volumenverteilungs-Kurven (1 00 Kanal-Spektrum) sind diese unter Weglassen der ersten 10 Kanale (Schmutz und Erythrocyten) in Groflenklassen (G) zu 5 Kanalen eingeteilt und in 6 Gruppen zusamniengefaflt worden, G 1-4, G 5-7, G 8-10, G 11-12, G 13-15 und G 16-18 (Abb.…”