Size distribution and chain conformation of six different fucoidans using size-exclusion chromatography with multiple detection

Neupane, Sandesh; Bittkau, Kaya Saskia; Alban, Susanne

doi:10.1016/j.chroma.2019.460658

Cited by 28 publications

(30 citation statements)

References 56 publications

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“…The molecular weight of crude fucoidans from F. evanescens has been previously reported to have various sizes from 181 to 400 kDa after extraction with aqueous CaCl 2 [ 47 ] and HCl purification [ 48 ]. In this study, the average size of crude fucoidans from F. evanescens was 200–400 kDa, which is a high molecular size when compared with fractions that were extracted by acid (from 150 to 500 kDa) [ 49 ] and by ultrasound-assisted extraction (280 and 240 kDa) [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

“…The fucoidans from S. latissima were more complex based on their monosaccharide composition. In addition, the SEC profiles for fraction SlF2 and SlF3 showed broad double peaks, suggesting that they might be large heterogeneous polysaccharides [ 47 ]. Three types of sulfated fucan-polysaccharides have been reported in S. latissima : fucogalactan, fucoglucuronomannan, and fucoglucuronan [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

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Enzyme-Assisted Fucoidan Extraction from Brown Macroalgae Fucus distichus subsp. evanescens and Saccharina latissima

et al. 2020

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Fucoidans from brown macroalgae (brown seaweeds) have different structures and many interesting bioactivities. Fucoidans are classically extracted from brown seaweeds by hot acidic extraction. Here, we report a new targeted enzyme-assisted methodology for fucoidan extraction from brown seaweeds. This enzyme-assisted extraction protocol involves a one-step combined use of a commercial cellulase preparation (Cellic®CTec2) and an alginate lyase from Sphingomonas sp. (SALy), reaction at pH 6.0, 40 °C, removal of non-fucoidan polysaccharides by Ca2+ precipitation, and ethanol-precipitation of crude fucoidan. The workability of this method is demonstrated for fucoidan extraction from Fucus distichus subsp. evanescens (basionym Fucus evanescens) and Saccharina latissima as compared with mild acidic extraction. The crude fucoidans resulting directly from the enzyme-assisted method contained considerable amounts of low molecular weight alginate, but this residual alginate was effectively removed by an additional ion-exchange chromatographic step to yield pure fucoidans (as confirmed by 1H NMR). The fucoidan yields that were obtained by the enzymatic method were comparable to the chemically extracted yields for both F. evanescens and S. latissima, but the molecular sizes of the fucoidans were significantly larger with enzyme-assisted extraction. The molecular weight distribution of the fucoidan fractions was 400 to 800 kDa for F. evanescens and 300 to 800 kDa for S. latissima, whereas the molecular weights of the corresponding chemically extracted fucoidans from these seaweeds were 10–100 kDa and 50–100 kDa, respectively. Enzyme-assisted extraction represents a new gentle strategy for fucoidan extraction and it provides new opportunities for obtaining high yields of native fucoidan structures from brown macroalgae.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Enzyme-Assisted Fucoidan Extraction from Brown Macroalgae Fucus distichus subsp. evanescens and Saccharina latissima

et al. 2020

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“…DF, also known as tubular net weed or golden sea hair, belongs to the order of Ectocarpales and is a highly branched brown seaweed species that grows on rocks, other algae, or is free-floating in North Atlantic and Northwest Pacific [23]. Literature on fucose containing sulfated polysaccharides from DF is so far limited to the mentioned DF extract [24,25]. Chemical characterization of the DF extract revealed that the applied fucoidan extraction and purification procedure did not result in fucoidans as known for many other brown algae, but a complex mixture of fucose containing polysaccharides and proteoglycans, respectively, with a relatively low sulfate content [24].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a Brown Seaweed Extract from Dictyosiphon foeniculaceus as a Potential Therapeutic Agent for the Treatment of Glioblastoma and Uveal Melanoma

et al. 2020

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Ingredients of brown seaweed like fucoidans are often described for their beneficial biological effects, that might be interesting for a medical application. In this study, we tested an extract from Dictyosiphon foeniculaceus (DF) to evaluate the effects in glioblastoma and uveal melanoma, looking for a possible anti-cancer treatment. We investigated toxicity, VEGF (vascular endothelial growth factor) secretion and gene expression of tumor and non-tumor cells. SVGA (human fetal astrocytes), the human RPE (retinal pigment epithelium) cell line ARPE-19, the tumor cell line OMM-1 (human uveal melanoma), and two different human primary glioblastoma cultures (116-14 and 118-14) were used. Tests for cell viability were conducted with MTS-Assay (3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium), and the proliferation rate was determined with cell counting. VEGF secretion was assessed with ELISA (enzyme-linked immunosorbent assay). The gene expression of VEGF receptor 1 (VEGFR1), VEGF receptor 2 (VEGFR2) and VEGF-A was determined with real-time qPCR (quantitative polymerase chain reaction). DF lowered the cell viability of OMM-1. Proliferation rates of ARPE-19 and OMM-1 were decreased. The VEGF secretion was inhibited in ARPE-19 and OMM-1, whereas it was increased in SVGA and 116-14. The expression of VEGFR1 was absent and not influenced in OMM-1 and ARPE-19. VEGFR2 expression was lowered in 116-14 after 24 h, whereas VEGF-A was increased in 118-14 after 72 h. The extract lowered cell viability slightly and was anti-proliferative depending on the cell type investigated. VEGF was heterogeneously affected. The results in glioblastoma were not promising, but the anti-tumor properties in OMM-1 could make them interesting for further research concerning cancer diseases in the human eye.

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“…The injection volume was 100 µL. The used dn/dc was 0.150 as previously described for fucoidans [17]. The data were collected and processed using the Astra (v.7.3.2) software (Wyatt Technology, Dernbach, Germany).…”

Section: Sec-mals-vis Analysismentioning

confidence: 99%

“…Before biomedical use of fucoidans, complex testing procedures are necessary. This is a challenging task, especially when considering the large number of extracts that can be gained from the different species of brown algae, as well as from different extraction methods or processing technologies, such as fractionation [10,16,17]. In this context, a detailed characterization of the molecular biological effects of fucoidans in the target cells will support the selection of extracts with the best biomedical effect for the envisioned therapeutic aim, contribute to their biological safety, and enable definition of structural and functional relationships at cellular and molecular levels.…”

Section: Introductionmentioning

confidence: 99%

Influence of Fucoidan Extracts from Different Fucus Species on Adult Stem Cells and Molecular Mediators in In Vitro Models for Bone Formation and Vascularization

et al. 2021

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Fucoidans, sulfated polysaccharides extracted from brown algae, are marine products with the potential to modulate bone formation and vascularization processes. The bioactivity and safety of fucoidans are highly associated with their chemical structure, which may vary with algae species and extraction method. Thus, in depth evaluation of fucoidan extracts in terms of endotoxin content, cytotoxicity, and their detailed molecular biological impact on the individual cell types in bone is needed. In this study, we characterized fucoidan extracts from three different Fucus species including Fucus vesiculosus (Fv), Fucus serratus (Fs), and Fucus distichus subsp. evanescens (Fe) for their chemical features, endotoxin content, cytotoxicity, and bioactive effects on human outgrowth endothelial cells (OEC) and human mesenchymal stem cells (MSC) as in vitro models for bone function and vascularization. Extracts contained mainly high molecular weight (HMW) fucoidans and were free of endotoxins that may cause inflammation or influence vascularization. OEC tolerated fucoidan concentrations up to 200 µg/mL, and no indication of cytotoxicity was observed. The inflammatory response, however, investigated by real-time PCR (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) and endothelial barrier assessed by impedance measurement differed for the individual extracts. MSC in comparison with endothelial cells were more sensitive to fucoidans and showed partly reduced metabolic activity and proliferation at higher doses of fucoidans. Further results for MSC indicated impaired osteogenic functions in alkaline phosphatase and calcification assays. All tested extracts consistently lowered important molecular mediators involved in angiogenesis, such a VEGF (vascular endothelial growth factor), ANG-1 (angiopoietin 1), and ANG-2 (angiopoietin 2), as indicated by RT-PCR and ELISA. This was associated with antiangiogenic effects at the functional level using selected extracts in co-culture models to mimic bone vascularization processes during bone regeneration or osteosarcoma.

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Size distribution and chain conformation of six different fucoidans using size-exclusion chromatography with multiple detection

Cited by 28 publications

References 56 publications

Enzyme-Assisted Fucoidan Extraction from Brown Macroalgae Fucus distichus subsp. evanescens and Saccharina latissima

Enzyme-Assisted Fucoidan Extraction from Brown Macroalgae Fucus distichus subsp. evanescens and Saccharina latissima

Evaluation of a Brown Seaweed Extract from Dictyosiphon foeniculaceus as a Potential Therapeutic Agent for the Treatment of Glioblastoma and Uveal Melanoma

Influence of Fucoidan Extracts from Different Fucus Species on Adult Stem Cells and Molecular Mediators in In Vitro Models for Bone Formation and Vascularization

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