Size Determination of Nanoparticles by Dynamic Light Scattering

Kato, Haruhisa

doi:10.1002/9783527646821.ch8

Cited by 4 publications

(2 citation statements)

References 48 publications

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“…In DLS, a relatively low NP concentration is needed so that a multiple scattering effect is avoided. 165 Lim et al have reviewed the characterization of NPs by DLS ( Fig. 2) focusing in the case of magnetic particles.…”

Section: X-ray-based Techniquesmentioning

confidence: 99%

Characterization techniques for nanoparticles: comparison and complementarity upon studying nanoparticle properties

2018

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Nanostructures have attracted huge interest as a rapidly growing class of materials for many applications. Several techniques have been used to characterize the size, crystal structure, elemental composition and a variety of other physical properties of nanoparticles. In several cases, there are physical properties that can be evaluated by more than one technique. Different strengths and limitations of each technique complicate the choice of the most suitable method, while often a combinatorial characterization approach is needed. In addition, given that the significance of nanoparticles in basic research and applications is constantly increasing, it is necessary that researchers from separate fields overcome the challenges in the reproducible and reliable characterization of nanomaterials, after their synthesis and further process (e.g. annealing) stages. The principal objective of this review is to summarize the present knowledge on the use, advances, advantages and weaknesses of a large number of experimental techniques that are available for the characterization of nanoparticles. Different characterization techniques are classified according to the concept/group of the technique used, the information they can provide, or the materials that they are destined for. We describe the main characteristics of the techniques and their operation principles and we give various examples of their use, presenting them in a comparative mode, when possible, in relation to the property studied in each case.

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Section: X-ray-based Techniquesmentioning

confidence: 99%

Characterization techniques for nanoparticles: comparison and complementarity upon studying nanoparticle properties

2018

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“…Compared to aggregation of nanoparticles, aggregation of ACP is a more complex process that proceeds simultaneously at significantly different length scales. A method widely used for following the aggregation of nanoparticles is dynamic light scattering (DLS), due to the fact that representative statistics can be obtained since the number of particles analyzed is much larger than that observed by microscopy techniques [30][31][32]. However, the drawback of DLS is that the intensity of light scattered from the particles is proportional to the sixth power of the particle radius.…”

Section: Introductionmentioning

confidence: 99%

Amorphous Calcium Phosphate Formation and Aggregation Process Revealed by Light Scattering Techniques

et al. 2018

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Amorphous calcium phosphate (ACP) attracts attention as a precursor of crystalline calcium phosphates (CaPs) formation in vitro and in vivo as well as due to its excellent biological properties. Its formation can be considered to be an aggregation process. Although aggregation of ACP is of interest for both gaining a fundamental understanding of biominerals formation and in the synthesis of novel materials, it has still not been investigated in detail. In this work, the ACP aggregation was followed by two widely applied techniques suitable for following nanoparticles aggregation in general: dynamic light scattering (DLS) and laser diffraction (LD). In addition, the ACP formation was followed by potentiometric measurements and formed precipitates were characterized by Fourier transform infrared spectroscopy (FTIR), powder X-ray diffraction (PXRD), transmission electron microscopy (TEM), and atomic force microscopy (AFM). The results showed that aggregation of ACP particles is a process which from the earliest stages simultaneously takes place at wide length scales, from nanometers to micrometers, leading to a highly polydisperse precipitation system, with polydispersity and vol. % of larger aggregates increasing with concentration. Obtained results provide insight into developing a way of regulating ACP and consequently CaP formation by controlling aggregation on the scale of interest.

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Mechanistic Understanding of Protein Corona Formation around Nanoparticles: Old Puzzles and New Insights

Nienhaus

2023

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Although a wide variety of nanoparticles (NPs) have been engineered for use as disease markers or drug delivery agents, the number of nanomedicines in clinical use has hitherto remained small. A key obstacle in nanomedicine development is the lack of a deep mechanistic understanding of NP interactions in the bio‐environment. Here, the focus is on the biomolecular adsorption layer (protein corona), which quickly enshrouds a pristine NP exposed to a biofluid and modifies the way the NP interacts with the bio‐environment. After a brief introduction of NPs for nanomedicine, proteins, and their mutual interactions, research aimed at addressing fundamental properties of the protein corona, specifically its mono‐/multilayer structure, reversibility and irreversibility, time dependence, as well as its role in NP agglomeration, is critically reviewed. It becomes quite evident that the knowledge of the protein corona is still fragmented, and conflicting results on fundamental issues call for further mechanistic studies. The article concludes with a discussion of future research directions that should be taken to advance the understanding of the protein corona around NPs. This knowledge will provide NP developers with the predictive power to account for these interactions in the design of efficacious nanomedicines.

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Size Determination of Nanoparticles by Dynamic Light Scattering

Cited by 4 publications

References 48 publications

Characterization techniques for nanoparticles: comparison and complementarity upon studying nanoparticle properties

Characterization techniques for nanoparticles: comparison and complementarity upon studying nanoparticle properties

Amorphous Calcium Phosphate Formation and Aggregation Process Revealed by Light Scattering Techniques

Mechanistic Understanding of Protein Corona Formation around Nanoparticles: Old Puzzles and New Insights

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