Size-dependent transfection efficiency of PEI-coated gold nanoparticles

Cebrián, Virginia; Martín-Saavedra, Francisco M.; Yagüe, Clara; Arruebo, Manuel; Santamarı́a, Jesús; Vilaboa, Nuria

doi:10.1016/j.actbio.2011.06.018

Cited by 87 publications

(65 citation statements)

References 44 publications

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“…2B and D). Cebrián et al [68] also found 6 nm PEI-coated Au NPs can load higher quantities of DNA than equivalent mass of 70 nm Au-PEI NPs because of the larger external surface area per unit mass. The incomplete complexation of DNA-Cy3 observed with L-LDH at 5:1 in culture media would be expected to reduce the cellular delivery of DNA-Cy3 to 50% of that using sLDH at the same particle number concentration.…”

Section: Effect Of Surface/internal-bulk Loading On Cellular Deliverymentioning

confidence: 99%

Particle size- and number-dependent delivery to cells by layered double hydroxide nanoparticles

Dong

Parekh

2015

Journal of Colloid and Interface Science

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a b s t r a c tIt is well known that delivery efficiency to cells is highly dependent on particle size and the administered dose. However, there is a marked discrepancy in many reports, mainly due to the inconsistency in assessment of various parameters. In this particular research, we designed experiments using layered double hydroxide nanoparticles (LDH NPs) to specifically elucidate the effect of particle size, dose and dye loading manner on cellular uptake. Using the number of LDH NPs taken up by HCT-116 cells as the indicator of delivery efficiency, we found that (1) the size of sheet-like LDH in the range of 40-100 nm did not significantly affect their cellular uptake; (2) cellular uptake of 40 and 100 nm LDH NPs was increased proportionally to the number concentration below a critical value, but remained relatively constant beyond the critical value; and (3) the effect of the dye loading manner is mainly dependent on the loading capacity or yield. In particular, the loading capacity is determined by the NP specific surface area. This research may be extended to a larger size range to examine the size effect, but suggests that it is necessary to set up a protocol to evaluate the effects of NP's physicochemical properties on the cellular delivery efficiency.

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Section: Effect Of Surface/internal-bulk Loading On Cellular Deliverymentioning

confidence: 99%

Particle size- and number-dependent delivery to cells by layered double hydroxide nanoparticles

Dong

Parekh

2015

Journal of Colloid and Interface Science

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“…Polyethylenimine (PEI) is reportedly useful for nucleic acid condensation and endosomal escape. 13 We previously showed that single-walled carbon nanotubes-PEI (SWNT-PEI) carriers allow the siRNA transfection of PC-3 cells and combined RNAi and photothermal therapy, which was more effective for killing cancer cells. 14 PEI application in vivo has been limited because the strong cationic charge limits the ability of nanocarriers to target therapeutic sites and reduces their stability in serum, thereby shortening the circulation time.…”

Section: Introductionmentioning

confidence: 99%

Magnetic Multi-Walled Carbon Nanotubes for Tumor Theranostics

Wang¹,

Shi²,

Hao³

et al. 2015

j biomed nanotechnol

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Current diagnostic techniques do not reliably detect cancer at early stages, and traditional chemotherapy lacks specificity and causes systemic toxicity. To address these issues, multifunctional nanomaterials are becoming more widely studied as a means of cancer detection, therapy, and monitoring. Here, iron oxide (Fe3O4) nanoparticles were conjugated onto the surface of multi-walled carbon nanotubes (MWNTs), which were then modified with polyethylenimine (PEI) and polyethylene glycol (PEG) to improve their solubility and biocompatibility. Finally, human telomerase reverse transcriptase (hTERT) siRNA was loaded on the MWNT surface by electrostatic interaction to obtain a multifunctional delivery system (MWNT-Fe3O4-PEI-PEG/siRNA). This delivery system efficiently delivered siRNA, allowed targeting of certain sites by magnetic fields, facilitated photothermal heating by near infrared irradiation, and enabled magnetic resonance imaging, thereby indicating great potential for cancer theranostic applications.

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“…For instance, various types of NPs including mesoporous silica NPs, 10 gold NPs, 48 PEI-coated gold NPs, 49 polymeric NPs, 50-52 quantum dots, 53 liposomes, 54 and LDH have been tested for delivering anticancer drugs or siRNA in osteosarcoma or Ewing's sarcoma cell lines. Furthermore, chitosan (CS) NPs were reported to encapsulate DNA enzyme, Dz13, to effectively inhibit osteosarcoma growth.…”

Section: Osteosarcomamentioning

confidence: 99%

Nanotechnology in the targeted drug delivery for bone diseases and bone regeneration

Wu²,

Chen³

et al. 2013

IJN

158

111

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Nanotechnology is a vigorous research area and one of its important applications is in biomedical sciences. Among biomedical applications, targeted drug delivery is one of the most extensively studied subjects. Nanostructured particles and scaffolds have been widely studied for increasing treatment efficacy and specificity of present treatment approaches. Similarly, this technique has been used for treating bone diseases including bone regeneration. In this review, we have summarized and highlighted the recent advancement of nanostructured particles and scaffolds for the treatment of cancer bone metastasis, osteosarcoma, bone infections and inflammatory diseases, osteoarthritis, as well as for bone regeneration. Nanoparticles used to deliver deoxyribonucleic acid and ribonucleic acid molecules to specific bone sites for gene therapies are also included. The investigation of the implications of nanoparticles in bone diseases have just begun, and has already shown some promising potential. Further studies have to be conducted, aimed specifically at assessing targeted delivery and bioactive scaffolds to further improve their efficacy before they can be used clinically.

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Size-dependent transfection efficiency of PEI-coated gold nanoparticles

Cited by 87 publications

References 44 publications

Particle size- and number-dependent delivery to cells by layered double hydroxide nanoparticles

Particle size- and number-dependent delivery to cells by layered double hydroxide nanoparticles

Magnetic Multi-Walled Carbon Nanotubes for Tumor Theranostics

Nanotechnology in the targeted drug delivery for bone diseases and bone regeneration

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