Size-dependent DNA mobility in nanochannels

Cross, Joshua D.; Strychalski, Elizabeth A.; Craighead, Harold G.

doi:10.1063/1.2757202

Cited by 87 publications

(124 citation statements)

References 32 publications

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“…This type of axial nanostructure has been applied to physical studies of DNA conformation and dynamics [10], diffusion [11], and electrokinetic mobility [12], as well as applications including DNA barcoding [13] and separation by analyte motion across boundaries formed by arrays of constrictions within a channel [14].…”

Section: Introductionmentioning

confidence: 99%

Nanofluidic structures with complex three-dimensional surfaces

2009

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Nanofluidic devices have typically explored a design space of patterns limited by a single nanoscale structure depth. A method is presented here for fabricating nanofluidic structures with complex three-dimensional (3D) surfaces, utilizing a single layer of grayscale photolithography and standard integrated circuit manufacturing tools. This method is applied to construct nanofluidic devices with numerous (30) structure depths controlled from ≈10 to ≈620 nm with an average standard deviation of <10 nm over distances of >1 cm. A prototype 3D nanofluidic device is demonstrated that implements size exclusion of rigid nanoparticles and variable nanoscale confinement and deformation of biomolecules.

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Section: Introductionmentioning

confidence: 99%

Nanofluidic structures with complex three-dimensional surfaces

2009

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“…However, the interaction between DNA and nanopore is still not well understood due to the small size of DNA/nanopore and dynamic translocation process. Studies on electrophoretic transportation of DNA in nanochannels have revealed significant contribution of DNAchannel surface interaction, which leads to a diffusion rate much lower than that predicted by traditional diffusion theory (47). Moreover, various chemical modifications are applied on nanopore surface for improved signal yield.…”

Section: Solid-state Nanoporesmentioning

confidence: 99%

Silicon-Based Novel Bio-Sensing Platforms at the Micro and Nano Scale

Liu

Iqbal

2009

ECS Trans.

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Microfabrication traditionally has been limited to silicon as demonstrated by the multi-billion dollar industry. The field is now understandably developing into a combination of physics, chemistry and biology in very non-traditional approaches to develop functional devices.Some examples are on-chip hybridization of deoxyribonucleic acid (DNA) and optical detection, DNA/protein/cell detection at the micro scale for diagnostics in medical, military, and food safety applications, etc. One area where silicon based nanotechnology can have an immediate and far reaching impact is sensing biological molecules and their processes, especially in genomics and proteomics. The promise of nanofabrication lies in the special ability to fabricate devices not only down to the scales of bacteria and viruses, but even down to the scales of the smallest biological entities, such as DNA! Nanotechnology and BioMEMS will have a significant impact on medicine and biology in the areas of single cell detection, diagnosis and combating disease, providing specificity of drug delivery for therapy, and avoiding time consuming steps to provide faster results and solutions to the patient. Integration of biology and silicon at the micro and nano scale offers tremendous opportunities for solving important problems in biology and medicine and enables a wide range of applications in diagnostics, therapeutics, and tissue engineering. This paper reviews state of the art in silicon-based bioMEMS and bionanotechnology as well as the future challenges and opportunities. A range of silicon devices integrating microsystems engineering with biology are discussed with focus on rapid detection of biological entities and development of point of care devices using electrical or mechanical phenomena at the micro and nano scale.

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“…The physical mechanism for the reduction purportedly results from the PVP either decreasing the absolute value of the zeta potential or increasing the viscosity within a Debye length from the silica boundary or a combination thereof. 30 The addition of PVP to nanoslits with depth on the order of 20 nm has been correlated 31,32 with modulated DNA mobility (high molecular weight PVP is used as sieving medium for this purpose 33 ). For some values of E av , the T4-PVP DNA mobility is larger than the T4-5x mobility and for other values it is lower.…”

Section: A Mobility Measurementsmentioning

confidence: 99%

Fluctuations of DNA mobility in nanofluidic entropic traps

Levy

2014

Biomicrofluidics

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We studied the mobility of DNA molecules driven by an electric field through a nanofluidic device containing a periodic array of deep and shallow regions termed entropic traps. The mobility of a group of DNA molecules was measured by fluorescent video microscopy. Since the depth of a shallow region is smaller than the DNA equilibrium size, DNA molecules are trapped for a characteristic time and must compress themselves to traverse the boundary between deep and shallow regions. Consistent with previous experimental results, we observed a nonlinear relationship between the mobility and electric field strength, and that longer DNA molecules have larger mobility. In repeated measurements under seemingly identical conditions, we measured fluctuations in the mobility significantly larger than expected from statistical variation. The variation was more pronounced for lower electric field strengths where the trapping time is considerable relative to the drift time. To determine the origin of these fluctuations, we investigated the dependence of the mobility on several variables: DNA concentration, ionic strength of the solvent, fluorescent dye staining ratio, electroosmotic flow, and electric field strength. The mobility fluctuations were moderately enhanced in conditions of reduced ionic strength and electroosmotic flow. V C 2014 AIP Publishing LLC. [http://dx

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Size-dependent DNA mobility in nanochannels

Cited by 87 publications

References 32 publications

Nanofluidic structures with complex three-dimensional surfaces

Nanofluidic structures with complex three-dimensional surfaces

Silicon-Based Novel Bio-Sensing Platforms at the Micro and Nano Scale

Fluctuations of DNA mobility in nanofluidic entropic traps

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