Size-Controlled Iron Oxide Nanoplatforms with Lipidoid-Stabilized Shells for Efficient Magnetic Resonance Imaging-Trackable Lymph Node Targeting and High-Capacity Biomolecule Display

Abstract

Nanoplatforms for biomolecule delivery to the lymph nodes have attracted considerable interest as vectors for immunotherapy. Core-shell iron oxide nanoparticles are particularly appealing because of their potential as theranostic magnetic resonance imaging (MRI)-trackable vehicles for biomolecule delivery. The key challenge for utilizing iron oxide nanoparticles in this capacity is control of their coating shells to produce particles with predictable size. Size determines both the carrier capacity for biomolecule display and the carrier ability to target the lymph nodes. In this study, we develop a novel coating method to produce core-shell iron oxide nanoparticles with controlled size. We utilize lipidlike molecules to stabilize self-assembled lipid shells on the surface of iron oxide nanocrystals, allowing the formation of consistent coatings on nanocrystals of varying size (10-40 nm). We further demonstrate the feasibility of leveraging the ensuing control of nanocarrier size for optimizing the carrier functionalities. Coated nanoparticles with 10 and 30 nm cores supported biomolecule display at 10-fold and 200-fold higher capacities than previously reported iron oxide nanoparticles, while preserving monodisperse sub-100 nm size populations. In addition, accumulation of the coated nanoparticles in the lymph nodes could be tracked by MRI and at 1 h post injection demonstrated significantly enhanced lymph node targeting. Notably, lymph node targeting was 9-40 folds higher than that for previously reported nanocarriers, likely due to the ability of these nanoparticles to robustly maintain their sub-100 nm size in vivo. This approach can be broadly applicable for rational design of theranostic nanoplatforms for image-monitored immunotherapy.

Antimicrobial and Physicochemical Properties of Artificial Saliva Formulations Supplemented with Core-Shell Magnetic Nanoparticles

“…Among many types of available nanoparticles, metallics, especially iron-oxide nanoparticles (NPs), have attracted more attention, due to their unique physicochemical properties and biocompatibility [25]. They are promising candidates in the biomedical application, including carriers in drug delivery systems, MRI-contrast agents, hyperthermia inducers and photosensitizers in the diagnostic and therapeutic areas [26,27]. Moreover, several studies proved their potential as antimicrobial agents that prevent biofilm formation [28,29].…”

Susceptibility of microbial cells to the modified PIP2-binding sequence of gelsolin anchored on the surface of magnetic nanoparticles

“…In the past decade, surface modified magnetic nanoparticles composed of an iron oxide core and a metallic or polymeric shell have received attention as potential nanomaterials that might be used in theranostic applications, for example as drug transporters, inducers of magnetic hyperthermia, MRI contrast agents and separators and trackers of macromolecules [1–4]. Many studies have focused on the development of an efficient and repeatable synthesis process to obtain size- and shape-controlled monodisperse and biocompatible nanoparticles [5]. For this purpose, different methods such as co-precipitation, thermal decomposition, microemulsion, sol–gel synthesis, and green synthesis using plant or bacterial extracts have been proposed [6].…”

Folic acid-conjugated magnetic mesoporous silica nanoparticles loaded with quercetin: a theranostic approach for cancer management

“…This isotherm could be classied as a type IV isotherm with two steep rises in the N 2 uptakes. 22,23 This capillary condensation is more pronounced in the desorption isotherm, which showed a large hysteresis. The BET surface area and pore volume of this FA-FE-SBA15QN material were 402 m 2 g À1 and 0.0737 cm 3 g À1 , respectively.…”

“…The small increase in the 2q values indicated a minor decrease in the dspacing values, which conrmed the surface functionalization of the SBA-15 base material. [21][22][23] In contrast, the broad peak in FA-FE-SBA15QN appears at a 2q value of 20-23 due to the amorphous nature of the SBA-15 material. Another broad peak and a sharp diffraction peak were observed at 2q values of 9.27-15.53 and 18.19 , respectively, corresponding to the folic acid moiety bound at the mesopore surface.…”