Size-based cytotoxicity of silver nanoparticles in bovine retinal endothelial cells

Sriram, Muthu Irulappan; Kalishwaralal, Kalimuthu; BarathManiKanth, Selvaraj; Gurunathani, Sangiliyandi

doi:10.1080/17458080.2010.547878

Cited by 72 publications

(43 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…ROS are a double-edged sword, as under normal physiological conditions a moderate level of ROS is essential to maintain normal physiological processes. However, excessive ROS, exceeding the capacity of the cellular antioxidant defense system, leads to cellular damage targeting proteins, lipids, and DNA [24,108,111,112,113]. Mitochondria are the main intracellular source of ROS.…”

Section: Mechanism Of Action Of Agnps In Various Cellular Systemsmentioning

confidence: 99%

Silver Nanoparticle-Mediated Cellular Responses in Various Cell Lines: An in Vitro Model

Zhang

Shen

Gurunathan

2016

IJMS

231

137

View full text Add to dashboard Cite

Silver nanoparticles (AgNPs) have attracted increased interest and are currently used in various industries including medicine, cosmetics, textiles, electronics, and pharmaceuticals, owing to their unique physical and chemical properties, particularly as antimicrobial and anticancer agents. Recently, several studies have reported both beneficial and toxic effects of AgNPs on various prokaryotic and eukaryotic systems. To develop nanoparticles for mediated therapy, several laboratories have used a variety of cell lines under in vitro conditions to evaluate the properties, mode of action, differential responses, and mechanisms of action of AgNPs. In vitro models are simple, cost-effective, rapid, and can be used to easily assess efficacy and performance. The cytotoxicity, genotoxicity, and biocompatibility of AgNPs depend on many factors such as size, shape, surface charge, surface coating, solubility, concentration, surface functionalization, distribution of particles, mode of entry, mode of action, growth media, exposure time, and cell type. Cellular responses to AgNPs are different in each cell type and depend on the physical and chemical nature of AgNPs. This review evaluates significant contributions to the literature on biological applications of AgNPs. It begins with an introduction to AgNPs, with particular attention to their overall impact on cellular effects. The main objective of this review is to elucidate the reasons for different cell types exhibiting differential responses to nanoparticles even when they possess similar size, shape, and other parameters. Firstly, we discuss the cellular effects of AgNPs on a variety of cell lines; Secondly, we discuss the mechanisms of action of AgNPs in various cellular systems, and try to elucidate how AgNPs interact with different mammalian cell lines and produce significant effects; Finally, we discuss the cellular activation of various signaling molecules in response to AgNPs, and conclude with future perspectives on research into AgNPs.

show abstract

Section: Mechanism Of Action Of Agnps In Various Cellular Systemsmentioning

confidence: 99%

Silver Nanoparticle-Mediated Cellular Responses in Various Cell Lines: An in Vitro Model

Zhang

Shen

Gurunathan

2016

IJMS

231

137

View full text Add to dashboard Cite

show abstract

“…Currently, nanobiotechnology is seen as means of producing the synthesis of nanoparticles an economical option for chemical and physical methods (1,2). Nanoparticles have unique chemical, physical and biological properties, and they are used in a lot of fields like therapeutics, cosmetics and drug delivery (3). Nanoparticles present a new perspective for detection, protection and treatment of tumors.…”

Section: Introductionmentioning

confidence: 99%

Silver nanoparticles; a new hope in cancer therapy?

Yeşilot¹,

Acar²

2019

Eastern J Med

View full text Add to dashboard Cite

Nowadays, with the rapid developments in nanotechnology, there has been a significant increase in the usage of nanoparticles within the biomedical field. Nanoparticles are known as particles ranging in size between 1 and 100 nm. Especially, silver nanoparticles (AgNPs) have been extensively carried out biomedical are as because of their physicochemical, and biological therapeutic properties. Recent studies have shown that there are anti-fungal, anti-viral, anti-inflammatory, anti-angiogenic biological activities, especially anti-bacterial and anti-cancer features of silver nanoparticles. Also, the appropriate knowledge of these characteristics is crucial since this knowledge enhances their potential usage in many areas while decreasing their possible dangers for human and environment. Recent researches demonstrate that multifunctional cytotoxic biological activities of biosynthesized silver nanoparticles can be used as an anti-cancer agent. In this work, it is aimed to give a literature review of the studies on silver nanoparticles, synthesis techniques and their possible usages especially in cancer therapy.

show abstract

“…Our studies are consistent with previously published reports suggesting that PDNPs and combination of TSA and PdNPs significantly increase the level of oxidative stress in human cervical cancer cells. 25,34,66,67 The ROS which increased as a result of combination treatment could easily react with membrane lipids, causing an alteration of membrane permeability, DNA damage and genomic instability, and loss of activity of enzymes and eventually proteins rendering the cells more susceptible to proteolytic degradation. 68 Some studies showed that rGO-Ag/TSA toxicity was accompanied by increased production of ROS and/or GSH depletion 34,71,72 Altogether, the elevated level of ROS causes the imbalance of oxidant and antioxidant levels in the cells, which is one of the reasons for cell death.…”

mentioning

confidence: 99%

Novel biomolecule lycopene-reduced graphene oxide-silver nanoparticle enhances apoptotic potential of trichostatin A in human ovarian cancer cells (SKOV3)

Zhang¹,

Huang²,

Zhang³

et al. 2017

IJN

View full text Add to dashboard Cite

Background Recently, there has been much interest in the field of nanomedicine to improve prevention, diagnosis, and treatment. Combination therapy seems to be most effective when two different molecules that work by different mechanisms are combined at low dose, thereby decreasing the possibility of drug resistance and occurrence of unbearable side effects. Based on this consideration, the study was designed to investigate the combination effect of reduced graphene oxide-silver nanoparticles (rGO-AgNPs) and trichostatin A (TSA) in human ovarian cancer cells (SKOV3). Methods The rGO-AgNPs were synthesized using a biomolecule called lycopene, and the resultant product was characterized by various analytical techniques. The combination effect of rGO-Ag and TSA was investigated in SKOV3 cells using various cellular assays such as cell viability, cytotoxicity, and immunofluorescence analysis. Results AgNPs were uniformly distributed on the surface of graphene sheet with an average size between 10 and 50 nm. rGO-Ag and TSA were found to inhibit cell viability in a dose-dependent manner. The combination of rGO-Ag and TSA at low concentration showed a significant effect on cell viability, and increased cytotoxicity by increasing the level of malondialdehyde and decreasing the level of glutathione, and also causing mitochondrial dysfunction. Furthermore, the combination of rGO-Ag and TSA had a more pronounced effect on DNA fragmentation and double-strand breaks, and eventually induced apoptosis. Conclusion This study is the first to report that the combination of rGO-Ag and TSA can cause potential cytotoxicity and also induce significantly greater cell death compared to either rGO-Ag alone or TSA alone in SKOV3 cells by various mechanisms including reactive oxygen species generation, mitochondrial dysfunction, and DNA damage. Therefore, this combination chemotherapy could be possibly used in advanced cancers that are not suitable for radiation therapy or surgical treatment and facilitate overcoming tumor resistance and disease progression.

show abstract

Size-based cytotoxicity of silver nanoparticles in bovine retinal endothelial cells

Cited by 72 publications

References 45 publications

Silver Nanoparticle-Mediated Cellular Responses in Various Cell Lines: An in Vitro Model

Silver Nanoparticle-Mediated Cellular Responses in Various Cell Lines: An in Vitro Model

Silver nanoparticles; a new hope in cancer therapy?

Novel biomolecule lycopene-reduced graphene oxide-silver nanoparticle enhances apoptotic potential of trichostatin A in human ovarian cancer cells (SKOV3)

Contact Info

Product

Resources

About