Size- and surface chemistry-dependent intracellular localization of luminescent silicon quantum dot aggregates

Ohta, Seiichi; Shen, Peng; Inasawa, Susumu; Yamaguchi, Yukio

doi:10.1039/c2jm31112g

Cited by 31 publications

(29 citation statements)

References 53 publications

(64 reference statements)

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“…Of these characteristics, the particle size is a crucial factor which determines NP endocytosis pathway [17,18], uptake rate and efficiency [6,16,[19][20][21][22], in vitro cytotoxicity [23][24][25][26][27][28], immune responses [27][28][29][30], and the final localisation [15,31,32]. Thus size-dependent uptake of various NPs has been intensively investigated and widely reported.…”

Section: Introductionmentioning

confidence: 99%

Particle size- and number-dependent delivery to cells by layered double hydroxide nanoparticles

Dong

Parekh

2015

Journal of Colloid and Interface Science

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a b s t r a c tIt is well known that delivery efficiency to cells is highly dependent on particle size and the administered dose. However, there is a marked discrepancy in many reports, mainly due to the inconsistency in assessment of various parameters. In this particular research, we designed experiments using layered double hydroxide nanoparticles (LDH NPs) to specifically elucidate the effect of particle size, dose and dye loading manner on cellular uptake. Using the number of LDH NPs taken up by HCT-116 cells as the indicator of delivery efficiency, we found that (1) the size of sheet-like LDH in the range of 40-100 nm did not significantly affect their cellular uptake; (2) cellular uptake of 40 and 100 nm LDH NPs was increased proportionally to the number concentration below a critical value, but remained relatively constant beyond the critical value; and (3) the effect of the dye loading manner is mainly dependent on the loading capacity or yield. In particular, the loading capacity is determined by the NP specific surface area. This research may be extended to a larger size range to examine the size effect, but suggests that it is necessary to set up a protocol to evaluate the effects of NP's physicochemical properties on the cellular delivery efficiency.

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Section: Introductionmentioning

confidence: 99%

Particle size- and number-dependent delivery to cells by layered double hydroxide nanoparticles

Dong

Parekh

2015

Journal of Colloid and Interface Science

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“…In addition to the above-mentioned exciting progress, SiNPs-based probes were recently also used to specifically target certain organelles, such as lysosomes, endoplasmic reticulum (ER), cytosol, and nuclei [32,33]. For example, Ohta and coworkers demonstrated that lysosomes and ER could be selectively labeled by surface modified SiNPs [33].…”

Section: In Vitro Imagingmentioning

confidence: 98%

“…For example, Ohta and coworkers demonstrated that lysosomes and ER could be selectively labeled by surface modified SiNPs [33]. In their work, the as-prepared SiNPs were treated In particular, the SiNPs/protein bioconjugates specifically target microtubules exhibiting red fluorescence.…”

Section: In Vitro Imagingmentioning

confidence: 99%

Silicon-Based Nanoprobes for Bioimaging Applications

He¹,

Su²

2014

SpringerBriefs in Molecular Science

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Bioimaging, serving as one of the most important techniques, provides direct visualization of biological systems. Biological probes are essential tools for bioimaging applications. Scientists have devoted tremendous efforts to developing various kinds of fluorescent nanomaterials (e.g., II-VI semiconductor quantum dots, fluorescent carbon nanodots, fluorescent nanodiamonds, silicon nanoparticles (SiNPs))-based bioprobes, significantly facilitating the advancement of bioimaging applications. Among them, SiNPs are regarded as potentially ideal fluorescent bioprobes due to their unique optical properties (e.g., high fluorescence and strong antiphotobleaching property) and excellent biocompatibility. In the past decade, SiNPs-based fluorescent bioprobes have been extensively explored for in vitro and in vivo imaging. Of particular significance, taking advantage of their ultrahigh photostability and non or lowly toxic properties, fluorescent SiNPs-based nanoprobes are demonstrated to be superbly suited to long-term and real-time bioimaging applications. Also of note, multifunctional SiNPs with fluorescent and magnetic properties have been developed for multimodel bioimaging studies in recent years.

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“…275,276 Pluronic F127 was also used as a dispersing agent for Si NCs produced by laser pyrolysis and capped with ethyl undecylenate. Luminescent and stable micelles were produced without preventive covalent surface capping of the Si NCs.…”

Section: Non-covalent Functionalizationmentioning

confidence: 99%

“…This method produced Si NC aggregates able to selectively label the endoplasmic reticulum (ER) in live cells. 276 In particular, size of the aggregates was controlled from ca. They form, in water, micelles where the PPG core constitutes a low polar nano-environment.…”

Section: Si Qds From the Plasma-assisted Processmentioning

confidence: 99%

Nanodiamonds and silicon quantum dots: ultrastable and biocompatible luminescent nanoprobes for long-term bioimaging

2015

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Fluorescence bioimaging is a powerful, versatile, method for investigating, both in vivo and in vitro, the complex structures and functions of living organisms in real time and space, also using super-resolution techniques. Being poorly invasive, fluorescence bioimaging is suitable for long-term observation of biological processes. Long-term detection is partially prevented by photobleaching of organic fluorescent probes. Semiconductor quantum dots, in contrast, are ultrastable, fluorescent contrast agents detectable even at the single nanoparticle level. Emission color of quantum dots is size dependent and nanoprobes emitting in the near infrared (NIR) region are ideal for low back-ground in vivo imaging. Biocompatibility of nanoparticles, containing toxic elements, is debated. Recent safety concerns enforced the search for alternative ultrastable luminescent nanoprobes. Most recent results demonstrated that optimized silicon quantum dots (Si QDs) and fluorescent nanodiamonds (FNDs) show almost no photobleaching in a physiological environment. Moreover in vitro and in vivo toxicity studies demonstrated their unique biocompatibility. Si QDs and FNDs are hence ideal diagnostic tools and promising non-toxic vectors for the delivery of therapeutic cargos. Most relevant examples of applications of Si QDs and FNDs to long-term bioimaging are discussed in this review comparing the toxicity and the stability of different nanoprobes.

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Size- and surface chemistry-dependent intracellular localization of luminescent silicon quantum dot aggregates

Cited by 31 publications

References 53 publications

Particle size- and number-dependent delivery to cells by layered double hydroxide nanoparticles

Particle size- and number-dependent delivery to cells by layered double hydroxide nanoparticles

Silicon-Based Nanoprobes for Bioimaging Applications

Nanodiamonds and silicon quantum dots: ultrastable and biocompatible luminescent nanoprobes for long-term bioimaging

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