Size and mass determination of silver nanoparticles in an aqueous matrix using asymmetric flow field flow fractionation coupled to inductively coupled plasma mass spectrometer and ultraviolet–visible detectors

Simultaneous Determination of Size and Quantification of Silica Nanoparticles by Asymmetric Flow Field-Flow Fractionation Coupled to ICPMS Using Silica Nanoparticles Standards

“…The limits of detection (LOD), calculated as three times the average signal of the background noise, are summarized in Table 3. The LODs obtained were slightly lower than the value reported by Dekkers and co-workers 34 for SiO 2 aqueous suspensions (1 mg L −1 ), and in general higher than values reported for the detection of NPs of a different nature by ICPMS, such as Ag-NPs 38,30 or AuNPs, 18 usually in μg L −1 level. The relatively high LOD for SiO 2 is an inherent consequence of the monitoring of isotope m/z 28, which is hampered by the natural occurrence of interfering species such as carbon monoxide and nitrogen.…”

“…This is in agreement with previously reported results on Ag-NPs. 30 However, such an explanation should be considered as hypothesis that could be confirmed in future experiments replacing the separation channel with a bypass connector. As a result of the observed differences in the recovery rates which vary with the particle size, it is important to note that quantification of samples containing SiO 2 −NPs by prechannel injection of NPs standards with a different particle size to that of the analyte sample, could lead to under/overestimate the concentration in test samples.…”

Exploring influences on the cellular uptake of medium-sized silver nanoparticles into THP-1 cells

“…Asymmetric flow field flow fraction (AF4)-inductively coupled plasma mass spectrometry (ICPMS) is applied for quantification of metallic ENPs [15][16][17]. Commonly, Ag ENPs in less complex matrices like aqueous matrices can be separated by AF4 [18,19]. To the best of our knowledge, only one publication is dealing about the analytical method of nanosilver in relation to cell line; in this case smaller (15 nm) ENPs and Ag(I) were studied with HepG2 cells by AF4-ICPMS [13].…”

Assessing silver nanoparticles behaviour in artificial seawater by mean of AF4 and spICP-MS

“…Samples were analysed on an AF4 system (Postnova Analytics) coupled to a UV detector, based on the method previously optimized for size fractionation of citrate stabilized AgNP from 20 up to100 nm described by Geiss (Geiss et al, 2013), varying the injected volume in order to compensate for the low concentration.…”