Size and complexity of polyadenylated RNAs induced in tobacco infected with sonchus yellow net virus

Rezaian, M. Ali; Heaton, Louis A.; Pedersen, Karl; Milner, Joel J.; Jackson, A.O.

doi:10.1016/0042-6822(83)90547-0

Cited by 31 publications

(32 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They have, however, been reported for the hordeivirus, barley stripe mosaic virus (Stanley et al, 1984) and for the negative sense plant rhabdovirus, sonchus yellow net virus (Rezaian et al, 1983). Tobacco etch virus has been reported to produce 10 minor polyadenylated RNA species (Otal & Hari, 1983), but these RNAs may be artefacts of the electrophoresis procedure (Dougherty, 1983).…”

Section: Discussionmentioning

confidence: 91%

Detection of Polyadenylated Subgenomic RNAs in Leaves Infected with the Potexvirus Daphne Virus X

Guilford

Forster

1986

Journal of General Virology

View full text Add to dashboard Cite

SUMMARYThe genomic RNA (6.0 kb) and five subgenomic RNAs of 4-9, 4.0, 2.1, 1.4 and 0-8 kb were detected by Northern hybridization in leaves ofNicotiana clevelandii infected with daphne virus X (DVX). Also, five species of dsRNA specific to DVX were extracted from infected leaves. In denaturing gels the dsRNAs gave bands with mobilities similar to those of the genomic RNA and the 4.9, 4.0, 2.1 and 0-8 kb subgenomic RNAs. The single-stranded RNAs apparently contain a tract of poly(A) as shown by oligo(dT)-cellulose chromatography and by an increased mobility on agarose gels following digestion with RNase H in the presence of oligo(dT)l 2-~ 8. When added to reticulocyte lysate, DVX RNA directed the synthesis of a 160000 mol. wt. (160K) protein and several smaller proteins. The coat protein (26K) was identified by immunoprecipitation with antiserum to DVX particles among the translation products of poly(A)-containing RNA isolated from infected leaves. This protein (26K) was translated from the smallest subgenomic RNA (0-8 kb) but was not translated from fulllength DVX RNA isolated by centrifugation through formamide-sucrose gradients.

show abstract

Section: Discussionmentioning

confidence: 91%

Detection of Polyadenylated Subgenomic RNAs in Leaves Infected with the Potexvirus Daphne Virus X

Guilford

Forster

1986

Journal of General Virology

View full text Add to dashboard Cite

show abstract

“…Total RNA was isolated using standard methods (6,21) modified by H. Klee (personal communication). Frozen pulverized plant material was extracted in a buffer containing 100 mm Tris-HCl (pH 7.6), 100 mM NaCl, 50 mM EGTA, 1% (w/v) SDS, 10 mm DTT, 6% (w/v) p-aminosalicylic acid (sodium salt), 1% (w/v) tri-isopropylnaphthalenesulfonic acid (sodium salt), with an equal volume of buffer-saturated phenol:chloroform:isoamyl alcohol (25:24:1, v/v/v).…”

mentioning

confidence: 99%

Cold Acclimation in Arabidopsis thaliana

Gilmour¹,

Hajela²,

Thomashow³

1988

Plant Physiol.

238

192

View full text Add to dashboard Cite

The abilities of two races ofArabidopsis thaliana L. (Heyn), Landsberg erecta and Columbia, to cold harden were examined. Landsberg, grown at 22 to 24°C, increased in freezing tolerance from an initial 50% lethal temperature (LT5o) of about -3°C to an LT5o of about -6°C after 24 hours at 4°C; LT5o values of -8 to -10°C were achieved after 8 to 9 days at 4°C. Similar increases in freezing tolerance were obtained with Columbia. In vitro translation of poly(A ) RNA isolated from control and coldtreated Columbia showed that low temperature induced changes in the population of translatable mRNAs. An mRNA encoding a polypeptide of about 160 kilodaltons (isoelectric point about 4.5) increased markedly after 12 to 24 h at 4°C, as did mRNAs encoding four polypeptides of about 47 kilodaltons (isoelectric points ranging from 5-5.5). Incubation of Columbia callus tissue at 4°C also resulted in increased levels of the mRNAs encoding the 160 kilodalton polypeptide and at least two of the 47 kilodalton polypeptides. In vivo labeling experiments using Columbia plants and callus tissue indicated that the 160 kilodalton polypeptide was synthesized in the cold and suggested that at least two of the 47 kilodalton polypeptides were produced. Other differences in polypeptide composition were also observed in the in vivo labeling experiments, some of which may be the result of posttranslational modifications of the 160 and 47 kilodalton polypeptides.Many plants become more resistant to freezing temperatures when first exposed to low nonfreezing temperatures, a process known as cold acclimation or cold hardening (11,23 (25). Plants were maintained in a controlled environment chamber at 22 to 24°C under constant illumination from cool white fluorescent lights. Light intensity was approximately 100 ,uE m 2 s-' PAR at soil level, and the RH was about 40 to 50%. Plants were subirrigated as necessary with distilled water. Cold treatment was at 4°C under constant illumination from cool-white fluorescent lights with light intensity ranging from approximately 20 to 50 ,uE m-2 s-' PAR, and the RH was between 75 and 95%. Experiments were performed before the plants had started to bolt, generally about 19 d after planting.For RNA extraction, rosette leaves and stems were excised at soil level, frozen in liquid N2, pulverized using a mortar and pestle, and stored at -80°C prior to extraction.Callus Culture. Arabidopsis (Columbia) seeds were surfacesterilized and placed on semisolid MSa2 medium (MSal) (17) and containing 3% (w/v)

show abstract

“…Alternatively, the RNA was Northern blotted to a Biodyne nylon membrane (Pall, Portsmouth, U.K.) and hybridized according to Gustafson et al (1982). The probe used was a mixture of plasmid DNAs (pSYN302, pSYN402 and pSYN503) containing inserts derived from the mRNAs for proteins N, MI and M2 (Rezaian et al, 1983;Ismail et al, 1987 and our unpublished data) These plasmids wer~ labelled by nick translation with [32P]dCTP to a specific activity of 1.1 × 108 d.p.m./gtg. cytoplasm.…”

Section: Methodsmentioning

confidence: 99%

Isolation of Defective Interfering Particles of Sonchus Yellow Net Virus from Chronically Infected Plants

Ismail

Milner

1988

Journal of General Virology

View full text Add to dashboard Cite

SUMMARYNicotiana edwardsonii plants were examined by electron microscopy 5 months after inoculation with Sonchus yellow net virus (SYNV). No virions were observed in leaf or root cells, but cells in sections of calyx tissue contained large numbers of virus particles. Most of these particles were only 73 to 86 ~ as tong as particles of standard SYNV. Nicotiana edwardsonii inoculated with sap extracted from chronically infected calyx became systemically infected but exhibited chlorotic mottling, instead of the normal vein-clearing symptoms. Most virus particles in these plants were short, and when purified, sedimented more slowly than standard SYNV. Purified short particles were not infective, but plants inoculated with a mixture of short and standard particles developed mottling symptoms and yielded predominantly short particles. Proteins from short particles were electrophoretically and antigenically identical to those from standard virus. RNA from short particles was 77~ the size of RNA from standard SYNV and hybridized to cloned SYNV cDNA. These short particles have all the characteristics of defective interfering particles.

show abstract

Size and complexity of polyadenylated RNAs induced in tobacco infected with sonchus yellow net virus

Cited by 31 publications

References 16 publications

Detection of Polyadenylated Subgenomic RNAs in Leaves Infected with the Potexvirus Daphne Virus X

Detection of Polyadenylated Subgenomic RNAs in Leaves Infected with the Potexvirus Daphne Virus X

Cold Acclimation in Arabidopsis thaliana

Isolation of Defective Interfering Particles of Sonchus Yellow Net Virus from Chronically Infected Plants

Contact Info

Product

Resources

About