Sixty polymorphic microsatellite markers for the oldfield mouse developed in <i>Peromyscus polionotus</i> and <i> Peromyscus maniculatus</i>

Mullen, Linda; Hirschmann, Rachel J.; Prince, Kelly L.; Glenn, Travis C.; Dewey, Michael J.; Hoekstra, Hopi E.

doi:10.1111/j.1471-8286.2005.01128.x

Cited by 22 publications

(21 citation statements)

References 18 publications

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“…First, we genotyped 212 individuals at 20 microsatellite markers (table S2 in the electronic supplementary material); primer sequences and reaction conditions are reported in Mullen et al (2006). Second, for 70 individuals (10-12 individuals from each subspecies, including two diverged populations within Choctawhatchee beach mice, and one from each of two outgroups), we sequenced two mitochondrial regions: ND3 and COIII (ND3-H and CO3-L; GenBank no.…”

Section: (C) Genetic Variationmentioning

confidence: 99%

Adaptive basis of geographic variation: genetic, phenotypic and environmental differences among beach mouse populations

et al. 2009

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A major goal in evolutionary biology is to understand how and why populations differentiate, both genetically and phenotypically, as they invade a novel habitat. A classical example of adaptation is the pale colour of beach mice, relative to their dark mainland ancestors, which colonized the isolated sandy dunes and barrier islands on Florida's Gulf Coast. However, much less is known about differentiation among the Gulf Coast beach mice, which comprise five subspecies linearly arrayed on Florida's shoreline. Here, we test the role of selection in maintaining variation among these beach mouse subspecies at multiple levels-phenotype, genotype and the environments they inhabit. While all beach subspecies have light pelage, they differ significantly in colour pattern. These subspecies are also genetically distinct: pair-wise F ST -values range from 0.23 to 0.63 and levels of gene flow are low. However, we did not find a correlation between phenotypic and genetic distance. Instead, we find a significant association between the average 'lightness' of each subspecies and the brightness of the substrate it inhabits: the two most genetically divergent subspecies occupy the most similar habitats and have converged on phenotype, whereas the most genetically similar subspecies occupy the most different environments and have divergent phenotypes. Moreover, allelic variation at the pigmentation gene, Mc1r, is statistically correlated with these colour differences but not with variation at other genetic loci. Together, these results suggest that natural selection for camouflage-via changes in Mc1r allele frequency-contributes to pigment differentiation among beach mouse subspecies.

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Section: (C) Genetic Variationmentioning

confidence: 99%

Adaptive basis of geographic variation: genetic, phenotypic and environmental differences among beach mouse populations

et al. 2009

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“…All samples were collected in accordance with regulations for threatened organisms and American Society of Mammalogists' guidelines (Sikes et al 2011). SEBM were genotyped using 10 microsatellite loci (PO2-40, PO3-59, PO-71, PO-116A, PO3-85, PO3-68, PPA-01, PO-31, PO2-23, PO-17) developed for Peromyscus polionotus (Wooten et al 1999;Prince et al 2002;Mullen et al 2006). Standard tissue extraction protocols were performed to isolate DNA from tail clippings using QIAGEN DNeasy Tissue Kits (Qiagen Inc., Valencia, CA).…”

Section: Tissue Sampling and Microsatellite Analysesmentioning

confidence: 99%

“…Standard tissue extraction protocols were performed to isolate DNA from tail clippings using QIAGEN DNeasy Tissue Kits (Qiagen Inc., Valencia, CA). Microsatellite loci were amplified using polymerase chain reaction protocols (Mullen et al 2006). Samples were sequenced on an ABI 3130 automated sequencer (Applied Biosystems, Culver City, CA).…”

Section: Tissue Sampling and Microsatellite Analysesmentioning

confidence: 99%

Microspatial sampling reveals cryptic influences on gene flow in a threatened mammal

et al. 2015

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We studied the population structure and historical demography of the last remaining core population of the threatened southeastern beach mouse (SEBM; Peromyscus polionotus niveiventris) located on a federally protected barrier island complex at the Kennedy Space Center (KSC), Merritt Island National Wildlife Refuge (MINWR) and Cape Canaveral Air Force Station (CCAFS) in Florida, USA. Beach mice (N = 171) were collected from 33 trapping locations along 30 km of coastline on KSC/MINWR/ CCAFS and were genotyped using 10 microsatellite loci. We found four genetic clusters of mice that likely form a metapopulation. Gene flow among clusters, assessed using assignment tests, was limited suggesting that human development can serve to inhibit dispersal of beach mice. However, when the presence of roads were examined as possible barriers to movement, gene flow appeared to be facilitated suggesting that removal of thick vegetation along roadsides increases movement. We used approximate Bayesian computation (ABC) to estimate divergence time among clusters and effective population sizes for each cluster and for the pre-divergence population. Results of ABC analyses suggest that barriers to movement likely formed following the construction of the John F. Kennedy Space Center beginning in the 1960s but that this has not heavily impacted the effective size of populations. Pre-divergence and contemporary effective sizes are similar, thus, population sizes likely remained relatively large over the last 50-100 years. The population of SEBM on KSC/MINWR/CCAFS appears to be a genetically diverse core population and individuals from this population will most likely be good candidates for any future reintroduction and translocation programs.

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“…This large set of microsatellites contributes to a growing number of genomic resources (Mullen et al 2006; Glenn et al 2008) for studies of Peromyscus evolution.…”

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“…Based on previous studies, it is likely that many of these markers will also amplify in additional Peromyscus species (Prince et al 2002) as well as other cricetid rodents. Moreover, even those markers that are not polymorphic in lab strains of Peromyscus are likely to be polymorphic in natural populations (Mullen et al 2006). Thus, this collection of markers will be useful for studies of ecological genetics in a large number of rodent taxa.…”

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Five hundred microsatellite loci for Peromyscus

et al. 2009

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Mice of the genus Peromyscus, including several endangered subspecies, occur throughout North America and have been important models for conservation research. We describe 526 primer pairs that amplify microsatellite DNA loci for P. maniculatus bairdii, 467 of which also amplify in P. polionotus subgriseus. For 12 of these loci, we report diversity data from a natural population. These markers will be an important resource for future genomic studies of Peromyscus evolution and mammalian conservation. Keywords microsatellite; Peromyscus maniculatus; Peromyscus polionotus; SSR; STR; PCR primersAs genomic markers, tools and techniques are becoming increasingly accessible, a handful of new "model" systems have emerged, allowing us to begin to better understand the evolution of organismal diversity (Abzhanov et al. 2008). One of these emerging models is Peromyscus (Cricetidae: Neotominae), arguably the most diverse and well-studied group of non-commensal rodents. Mice of the genus Peromyscus are ubiquitous throughout North America with more than 50 species distributed across a variety of habitats (Hall 1981). The most widespread species, the deer mouse Peromyscus maniculatus, has been the subject of conservation, ecological and evolutionary studies starting in the early 1900 's (e.g., Osgood 1909Dice 1940;Sullivan 1977;Jimenez et al. 1994;Storz et al. 2007) and is considered a model organism for such research (Dewey and Dawson 2001). Peromyscus maniculatus is comprised of more than 65 subspecies that display a wide range of morphological and behavioral variation (King 1968;Hall 1981). In addition, as a carrier of both Lyme disease (Spielman et al. 1985) and hantavirus (Childs et al. 1994), P. maniculatus recently emerged as a health concern and warrants continued research and monitoring for environmental and human health effects.Correspondence: Travis C. Glenn, Department of Environmental Health Science, University of Georgia, Athens, GA 30602 USA. travisg@uga.edu. NIH Public Access Author ManuscriptConserv Genet. Author manuscript; available in PMC 2011 June 1. NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author ManuscriptThe maniculatus species group includes several geographically peripheral taxa, including its sister species, the oldfield mouse Peromyscus polionotus (Blair 1950;Avise et al. 1983;Bradley et al. 2007). Blair (1950) proposed that P. polionotus split from P. maniculatus in the Pleistocene, and populations of P. polionotus were isolated to the southeastern states and along the Gulf and Atlantic coasts. P. polionotus has sixteen subspecies (Hall 1981), eight of which are 'beach mice' that occur along the sandy dunes and barrier islands of the Atlantic and Gulf coast (Bowen 1968;Bowen and Dawson 1977). Six of these eight subspecies are classified as endangered or threatened and one, P. p. decoloratus, is now considered extinct (USFWS 1999). Subspecies of P. polionotus exhibit broad variation in pelage coloration (Bowen 1968;Mullen and Hoekstra 2008) and frequently match their loca...

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Sixty polymorphic microsatellite markers for the oldfield mouse developed in Peromyscus polionotus and Peromyscus maniculatus

Cited by 22 publications

References 18 publications

Adaptive basis of geographic variation: genetic, phenotypic and environmental differences among beach mouse populations

Adaptive basis of geographic variation: genetic, phenotypic and environmental differences among beach mouse populations

Microspatial sampling reveals cryptic influences on gene flow in a threatened mammal

Five hundred microsatellite loci for Peromyscus

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