“…It is evident from the data in table 1 that the ICG fluorescence in D,O and CH,OD are both significantly higher than in H,O and CH,OH, respectively. This behaviour was observed by Webb, Webster and Plourde (1974) in other dyes that are similar to ICG, and a quenching mechanism has been proposed by Drexhage (1973a, b). However, these solvent-dye interactions were found to be of secondary importance to dye-dye and dye-protein interactions.…”