Six1 homeoprotein drives myofiber type IIA specialization in soleus muscle

Sakakibara, Iori; Wurmser, Maud; Santos, Matthieu Dos; Santolini, Marc; Ducommun, Serge; Davaze, Romain; Guernec, Anthony; Sakamoto, Kei; Maire, Pascal

doi:10.1186/s13395-016-0102-x

Cited by 26 publications

(28 citation statements)

References 77 publications

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“…In adult fast‐type muscles, Six1 functions as a main mediator of fast‐fibre acquisition and maintenance . Deletion of Six1 in soleus muscles impaired fast‐type myofiber specialization , while overexpressing Six1 and eyes absent 1 (Eya1) in the adult slow‐twitch muscle can induce a transformation from slow‐twitch to fast‐twitch fibre type .…”

Section: Mechanisms Involved In Controlling Myofiber Types During Embmentioning

confidence: 88%

Metabolic control of myofibers: promising therapeutic target for obesity and type 2 diabetes

Duan

Tan

et al. 2017

Obesity Reviews

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Mammalian skeletal muscles are composed of two major fibre types (I and II) that differ in terms of size, metabolism and contractile properties. In general, slow-twitch type I fibres are rich in mitochondria and have a greater insulin sensitivity than fast-twitch type II skeletal muscles. Although not widely appreciated, a forced induction of the slow skeletal muscle phenotype may inhibit the progress of obesity and diabetes. This potentially forms the basis for targeting slow/oxidative myofibers in the treatment of obesity. In this context, a better understanding of the molecular basis of fibre-type specification and plasticity may help to identify potential therapeutic targets for obesity and diabetes.

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Section: Mechanisms Involved In Controlling Myofiber Types During Embmentioning

confidence: 88%

Metabolic control of myofibers: promising therapeutic target for obesity and type 2 diabetes

Duan

Tan

et al. 2017

Obesity Reviews

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“…This suggests that the expression of Myh1 and Myh2 in the soleus is less under the control of innervation than the expression of Myh4 in fast muscles. Knowing that in adult myofibers the homeoprotein SIX1 controls the expression of many fast/glycolytic muscle genes among which fMyh genes (Sakakibara et al, 2014;Sakakibara et al, 2016) and that SIX binding motifs accessibility are enriched in Myh4 myonuclei ( (Ramachandran et al, 2019), Figure 3C), we looked at the nuclear accumulation of this protein in fast myonuclei along EDL myofibers.…”

Section: Innervation Controls Myonuclei Coordination Along the Myofibermentioning

confidence: 99%

Single-nucleus RNA-seq and FISH reveal coordinated transcriptional activity in mammalian myofibers

Santos

Backer

Saintpierre

et al. 2020

Preprint

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Skeletal muscle fibers are large syncytia but it is currently unknown whether gene expression is coordinately regulated in their numerous nuclei. By snRNA-seq and snATAC-seq, we showed that slow, fast, myotendinous and neuromuscular junction myonuclei each have different transcriptional programs, associated with distinct chromatin states and combinations of transcription factors. In adult mice, identified myofiber types predominantly express either a slow or one of the three fast isoforms of Myosin heavy chain (MYH) proteins, while a small number of hybrid fibers can express more than one MYH. By snRNA-seq and FISH, we showed that the majority of myonuclei within a myofiber are synchronized, coordinately expressing only one fast Myh isoform with a preferential panel of muscle-specific genes. Importantly, this coordination of expression occurs early during post-natal development and depends on innervation. These findings highlight a unique mechanism of coordination of gene expression in a syncytium. Introduction:

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“…It has been found that white streak muscles have up-regulated expression of striated muscle contraction-related genes compared with normal meat [40]. Six1 converts slow muscle fibres into fast muscle fibres [41,42]. The proportion of fast muscle fibres was negatively correlated with post-mortem pH [43].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis reveals a molecular understanding of nicotinamide and butyrate sodium on meat quality of broilers under high stocking density

Wang

Yin

et al. 2020

BMC Genomics

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Background: In recent years, increased attention has been focused on breast muscle yield and meat quality in poultry production. Supplementation with nicotinamide and butyrate sodium can improve the meat quality of broilers. However, the potential molecular mechanism is not clear yet. This study was designed to investigate the effects of supplementation with a combination of nicotinamide and butyrate sodium on breast muscle transcriptome of broilers under high stocking density. A total of 300 21-d-old Cobb broilers were randomly allocated into 3 groups based on stocking density: low stocking density control group (L; 14 birds/m 2), high stocking density control group (H; 18 birds/m 2), and high stocking density group provided with a combination of 50 mg/kg nicotinamide and 500 mg/kg butyrate sodium (COMB; 18 birds/m 2), raised to 42 days of age. Results: The H group significantly increased cooking losses, pH decline and activity of lactate dehydrogenase in breast muscle when compared with the L group. COMB showed a significant decrease in these indices by comparison with the H group (P < 0.05). The transcriptome results showed that key genes involved in glycolysis, proteolysis and immune stress were up-regulated whereas those relating to muscle development, cell adhesion, cell matrix and collagen were down-regulated in the H group as compared to the L group. In contrast, genes related to muscle development, hyaluronic acid, mitochondrial function, and redox pathways were up-regulated while those associated with inflammatory response, acid metabolism, lipid metabolism, and glycolysis pathway were down-regulated in the COMB group when compared with the H group. Conclusions: The combination of nicotinamide and butyrate sodium may improve muscle quality by enhancing mitochondrial function and antioxidant capacity, inhibiting inflammatory response and glycolysis, and promoting muscle development and hyaluronic acid synthesis.

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Six1 homeoprotein drives myofiber type IIA specialization in soleus muscle

Cited by 26 publications

References 77 publications

Metabolic control of myofibers: promising therapeutic target for obesity and type 2 diabetes

Metabolic control of myofibers: promising therapeutic target for obesity and type 2 diabetes

Single-nucleus RNA-seq and FISH reveal coordinated transcriptional activity in mammalian myofibers

Transcriptome analysis reveals a molecular understanding of nicotinamide and butyrate sodium on meat quality of broilers under high stocking density

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