Six phenylalanine ammonia-lyases from Camellia sinensis: Evolution, expression, and kinetics

Wu, Ying-Ling; Wang, Wenzhao; Li, Yanzhi; Dai, Xinlong; Ma, Guoliang; Xing, Dawei; Zhu, Mengqing; Gao, Liping; Xia, Tao

doi:10.1016/j.plaphy.2017.06.030

Cited by 40 publications

(27 citation statements)

References 48 publications

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“…Previous research shown that NnPAL1 as an ancient member of the PAL family, and was found to be a polybasic origin in the evolution of PAL in angiosperms (Wu et al, 2014; Wu et al, 2017). To investigate the phylogenetic relationships of PbPAL s with other plants and bacterias, which a neighbor-joining tree was created.…”

Section: Resultsmentioning

confidence: 98%

“…Gene duplication events not only can promote the functional differentiation of PAL family during plant growth and development, but also PAL family under abiotic stress (Wu et al, 2017). For example, only AtPAL1 and AtPAL2 have functional specificity for nitrogen deficiency and low temperature in A. thaliana (Olsen et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…At present, the PAL family is screened and identified in A. thaliana , Camellia sinensis and other plants, and their critical roles in the formation of catechins, flavonols and their derivatives have also been clarified (Wu et al, 2017). However, genome-wide analysis of the PAL family in Rosaceae plants is rarely reported.…”

Section: Introductionmentioning

confidence: 99%

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Comparative genomic analysis of the PAL genes in five Rosaceae species and functional identification of Chinese white pear

Wang

Cheng

et al. 2019

PeerJ

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Phenylalanine ammonia lyase (PAL) plays an important role in the biosynthesis of secondary metabolites regulating plant growth response. To date, the evolutionary history of the PAL family in Rosaceae plants remains unclear. In this study, we identified 16 PAL homologous genes in five Rosaceae plants (Pyrus bretschneideri, Fragaria vesca, Prunus mume, Prunus persica, and Malus × domestica). We classified these PALs into three categories based on phylogenetic analysis, and all PALs were distributed on 13 chromosomes. We tracked gene duplication events and performed sliding window analysis. These results revealed the evolution of PALs in five Rosaceae plants. We predicted the promoter of the PbPALs by PLANT CARE online software, and found that the promoter region of both PbPAL1 and PbPAL3 have at least one AC element. The results of qRT-PCR analysis found that PbPAL1 and PbPAL2 were highly expressed in the stems and roots, while expression level of PbPAL3 was relatively low in different tissues. The expression of PbPAL1 and PbPAL2 increased firstly and then decreased at different developmental periods of pear fruit. Among them, the expression of PbPAL1 reached the highest level 55 days after flowering. Three PbPALs were induced by abiotic stress to varying degrees. We transfected PbPAL1 and PbPAL2 into Arabidopsis thaliana, which resulted in an increase in lignin content and thickening of the cell walls of intervascular fibres and xylem cells. In summary, this research laid a foundation for better understanding the molecular evolution of PALs in five Rosaceae plants. Furthermore, the present study revealed the role of PbPALs in lignin synthesis, and provided basic data for regulating lignin synthesis and stone cells development in pear plants. . 2019. Comparative genomic analysis of the PAL genes in five Rosaceae species and functional identification of Chinese white pear. PeerJ 7:e8064 http://doi.Chapple C. 2015. Manipulation of guaiacyl and syringyl monomer biosynthesis in an Arabidopsis cinnamyl alcohol dehydrogenase mutant results in atypical lignin biosynthesis and modified cell wall structure.

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Section: Resultsmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

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Comparative genomic analysis of the PAL genes in five Rosaceae species and functional identification of Chinese white pear

Wang

Cheng

et al. 2019

PeerJ

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show abstract

“…These results suggested that the genetic diversity and functional differentiation of PbPAL genes are necessary for plants to adapt to the environment. Not only can gene replication events promote the functional differentiation of PAL family during plant growth and development, but also PAL gene family under abiotic stress (Wu et al, 2017). For example, only AtPAL1 and AtPAL2 have functional specificity for nitrogen deficiency and low temperature in A. thaliana (Olsen et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…In a recent study shown that NnPAL1 as an ancient member of the PAL gene family, and was found to be a polybasic origin in the evolution of PAL in angiosperms (Wu et al, 2014;Wu et al, 2017). To investigate the phylogenetic relationships of PbPAL genes with other plants PAL genes, which a neighbor-joining tree was created.…”

Section: Phylogenetic Analysis Of Pal Genes In Pear and Other Plantsmentioning

confidence: 99%

Genome-wide analysis of phenylalanine ammonia-lyase (PAL) gene family in five Rosaceae plants and expression analysis and functional identification of Chinese white pear

Wang

Xiong

et al. 2019

Preprint

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Phenylalanine ammonia lyase (PAL) plays an important role in the biosynthesis of secondary metabolites regulating plant growth response. To date, the evolutionary history of the PAL family in Rosaceae plants remains unclear. In this study, we identified 16 PAL homologous genes in five Rosaceae plants (Pyrus bretschneideri, Fragaria vesca, Prunus mume, Prunus persica, and Malus × domestica). We classified these PAL genes into three categories based on phylogenetic analysis，and all PAL genes were distributed on 13 chromosomes. Subsequently, we track gene replication events and perform sliding window analysis. These results revealed the evolution of PAL genes in five Rosaceae plants. We predicted the promoter of the PbPAL genes by PLANTR CARE online software, and found that the 5'regulatory region of both PbPAL1 and PbPAL3 has at least one AC element motif. The results of qRT-PCR analysis found that PbPAL1 and PbPAL2 were highly expressed in the stem and root , while expression level of PbPAL3 was relatively low in different tissues. The expression of PbPAL1 and PbPAL2 genes increased firstly and then decreased at different developmental periods of pear fruit. Among them, the expression of PbPAL1 reached the highest level 55 days after flower. Three PbPAL genes were induced by abiotic stress to varying degrees. We transfected PbPAL1 and PbPAL2 genes into Arabidopsis thaliana, resulting in lignin content increased significantly and thick cell wall of intervascular fibers and xylem cells. In summary, This study laid a foundation for better understanding the molecular evolution of PAL genes in five Rosaceae plants. Furthermore, the present study revealed the role of PbPAL genes in lignin synthesis, and provided basic data for regulating lignin synthesis and stone cell development of pear.

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Recent Advances in Seedling Research: Phenylalanine and Phenylalanine Ammonia-Lyase (PAL)

Warpeha

2024

Progress in Botany

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Six phenylalanine ammonia-lyases from Camellia sinensis: Evolution, expression, and kinetics

Cited by 40 publications

References 48 publications

Comparative genomic analysis of the PAL genes in five Rosaceae species and functional identification of Chinese white pear

Comparative genomic analysis of the PAL genes in five Rosaceae species and functional identification of Chinese white pear

Genome-wide analysis of phenylalanine ammonia-lyase (PAL) gene family in five Rosaceae plants and expression analysis and functional identification of Chinese white pear

Recent Advances in Seedling Research: Phenylalanine and Phenylalanine Ammonia-Lyase (PAL)

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