Six New Steroidal Saponins from Helleborus thibetanus

Zhang, Hui; Su, Yi; Yang, Fan; Zhao, Ze-Qing; Gao, Xiumei

doi:10.1002/hlca.201400080

Cited by 9 publications

(2 citation statements)

References 16 publications

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“…9) Recently, various bufadienolides from H. orientalis and H. foetidus were isolated and identified, some of which showed significantly potent cytotoxic activity and induced apoptosis via a mitochondria dependent pathway in cultured tumor cells. 10,11) Because Helleborus species has been reported to concomitantly contain the glycoside derivatives of C 27 steroids (spirostanols and furostanols), [12][13][14][15][16][17][18] further phytochemical examination of the methanolic extract of H. foetidus was performed using whole plants with a specific focus on steroidal glycosides. The isolation of 28 steroidal glycosides , including 20 novel spirostanol glycosides (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20) and a novel furostanol glycoside (21) was achieved.…”

Section: Introductionmentioning

confidence: 99%

Novel Steroidal Glycosides from the Whole Plants of Helleborus foetidus

et al. 2020

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Phytochemical analysis of the whole Helleborus foetidus plants identified 28 steroidal glycosides (1-28), including 20 novel spirostanol glycosides (1-20) and a novel furostanol glycoside (21). The structures of the newly identified compounds were elucidated by two-dimensional NMR spectroscopy and hydrolytic cleavage. Compounds 12, 13, and 15 were determined to be spirostanol trisdesmosides bearing sugar moieties at the C-1,-21, and-24 hydroxy groups of the aglycone unit. The isolated compounds were subsequently evaluated for cytotoxic activity against HL-60 human promyelocytic leukemia cells and A549 human lung carcinoma cells. In particular, 7 showed cytotoxic activity against the HL-60 and A549 cells, with IC 50 values of 5.9 and 6.6 µM, respectively, whereas 19 was selectively cytotoxic to A549 cells with an IC 50 value of 5.5 µM.

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Section: Introductionmentioning

confidence: 99%

Novel Steroidal Glycosides from the Whole Plants of Helleborus foetidus

et al. 2020

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“…It is also called “Tie Kuai Zi” or “Xiao Tao Er Qi” locally . The roots and rhizomes of H. thibetanus are ethnopharmacologically useful in the treatment of asthma, cystitis, injury, urethritis, sores, traumatic injury, and occasionally cancers. , In traditional Chinese medicine, H. thibetanus is believed to have the abilities of clearing heat, detoxifying and activating blood circulation, dissipating blood stasis, detumescence, and pain relief . The chemical constituents and biological activities of H. thibetanus have attracted a broad attention.…”

Section: Introductionmentioning

confidence: 99%

Cardiac Glycoside Compound Isolated from Helleborus thibetanus Franch Displays Potent Toxicity against HeLa Cervical Carcinoma Cells through ROS-Independent Autophagy

Man

Wang

Zhou

et al. 2019

Chem. Res. Toxicol.

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The current study aimed to examine the anticancer activity of HTF-1, a cardiac glycoside (CG) isolated from Helleborus thibetanus Franch, using a cell-based model and to discover the underlying mechanisms with specific focus on autophagy. We found that HTF-1 was able to potently decrease the viability of several cancer cell lines especially for HeLa cervical carcinoma cells. It was discovered that HTF-1 dose dependently induced overproduction of ROS in HeLa cells, and the cell viability can be rescued when adding ROS scavenger N-acetyl-L-cysteine (NAC). More, we found that HTF-1 induced ROS-independent autophagy in concentration-and time-dependent manners in HeLa cells. This can be collectively verified by LC3-II and p62 abundance and also eGFP-LC3 puncta assay, bafilomycin clamp experiment, and acidotropic dye fluorescent labeling experiment. Additionally, TEM examination showed more autophagic vacuoles for HTF-1-treated HeLa cells. In HeLa cells, pretreatment with wortmannin (an inhibitor of the initial stages of autophagy to block autophagosome formation, thus, it should weaken the autophagy induction effect of HTF-1) decreased the autophagic flux and partially antagonized cell death induced by HTF-1, indicating that autophagy induced by HTF-1 played a cancer-suppressing role. Furthermore, coadministration of BAF (as a distal inhibitor of autophagy) with HTF-1 demonstrated a synergistic anticancer effect against HeLa cells. We believe that our work will enrich the understanding of CGs and especially anticarcinoma activity, also, pave the way for natural-product-based anticancer drug development.

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A cardiac glycoside HTF-1 isolated from Helleborus thibetanus Franch displays potent in vitro anti-cancer activity via caspase-9, MAPK and PI3K-Akt-mTOR pathways

Meng

et al. 2018

European Journal of Medicinal Chemistry

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Six New Steroidal Saponins from Helleborus thibetanus

Cited by 9 publications

References 16 publications

Novel Steroidal Glycosides from the Whole Plants of <i>Helleborus foetidus</i>

Novel Steroidal Glycosides from the Whole Plants of <i>Helleborus foetidus</i>

Cardiac Glycoside Compound Isolated from Helleborus thibetanus Franch Displays Potent Toxicity against HeLa Cervical Carcinoma Cells through ROS-Independent Autophagy

A cardiac glycoside HTF-1 isolated from Helleborus thibetanus Franch displays potent in vitro anti-cancer activity via caspase-9, MAPK and PI3K-Akt-mTOR pathways

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