In this paper, we present a method for achieving precise evaluation of amplitude of refractive index modulation (RIM) inside the volume Bragg grating (VBG) recorded in photo-thermo-refractive (PTR) glasses. The Gaussian divergence characteristics of the incident beam is theoretically considered when calculating the angular selectivity of VBG, and the profiles of experimental angular selectivity curves are utilized to determine the value of RIM with one step. The effectiveness of our proposed method is experimentally verified. This method is applicable even if the full width at half maximum (FWHM) of VBG's angular selectivity curve has the same order of magnitude as or is less than the beam divergence.