1987
DOI: 10.1093/nar/15.16.6315
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Site specificity of bleomycin cleavage in purified and intracellular simian virus 40 DNA

Abstract: The sites of bleomycin-induced cleavage of purified and intracellular simian virus 40 DNA were examined. Breaks in purified DNA were mapped to several discrete sites that were distributed throughout the viral genome, but were not associated with a conmnon genetic element. Double-stranded breaks were made in positions of the first single-stranded nick, and regions of cuts were unaffected by changes In DNA superhelicity. Bleomycin cut intracellular chromosomes at the same sites that were cleaved in purified DNA.… Show more

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Cited by 19 publications
(9 citation statements)
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References 26 publications
(32 reference statements)
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“…African green monkey kidney BSC-1 cells (from Dr. Terry Beerman, Roswell Park Cancer Inst., Buffalo, NY) were cultured as described previously (24,27). When drug effects on intracellular SV40 DNA were examined, cells were infected with SV40 virus as described (24,27,28) and at 40 h postinfection treated with drug for 4 h at 37 °C. SV40 DNA was then isolated as described previously (28).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…African green monkey kidney BSC-1 cells (from Dr. Terry Beerman, Roswell Park Cancer Inst., Buffalo, NY) were cultured as described previously (24,27). When drug effects on intracellular SV40 DNA were examined, cells were infected with SV40 virus as described (24,27,28) and at 40 h postinfection treated with drug for 4 h at 37 °C. SV40 DNA was then isolated as described previously (28).…”
Section: Methodsmentioning
confidence: 99%
“…When drug effects on intracellular SV40 DNA were examined, cells were infected with SV40 virus as described (24,27,28) and at 40 h postinfection treated with drug for 4 h at 37 °C. SV40 DNA was then isolated as described previously (28).…”
Section: Methodsmentioning
confidence: 99%
“…(African green monkey kidney) cells was in Minimal Essential Medium plus 10% calf serum (MEM), and infection with 5-15 plaque-forming units of SV40 virus per 0.6 x 106 cells was as described elsewhere (Grimwade et al, 1987).…”
Section: Methodsmentioning
confidence: 99%
“…Maintenance of BSC-I (African green monkey kidney) cells was in Minimal Essential Medium, 10% calf serum (MEM). Infection with 5-15 plaque-forming units of SV40 virus per 6 X 10 5 cells was as described elsewhere [14]. BSC-1 cells at 6 X 10 5 per 35 mm plate were infected as described above with SV40 virus.…”
Section: Cleurlage Of Intracellular Dna B! the Holoatrtibioticmentioning
confidence: 99%