Site‐specific N‐terminal Auto‐degradation of Human Serum Albumin

Chan, Bernard; Dodsworth, Neil; Woodrow, John R.; Tucker, Alan; Harris, R.

doi:10.1111/j.1432-1033.1995.tb20419.x

Cited by 103 publications

(59 citation statements)

References 14 publications

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“…Generation of ROS can transiently modify the N-terminal region of albumin and produce an increase in IMA levels (23,24). Some authors (24,25) reported that ROS generation in vitro causes structural changes in a synthetic N-terminus tetrapeptide, an octapeptide, and human albumin with loss of Co 21 binding capacity. High or low albumin concentrations may affect IMA testing, producing lower or higher values, respectively, even within the reference interval.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of ischemia‐modified albumin in anemia associated to chronic kidney disease

Cichota

Moresco

Duarte

et al. 2008

Clinical Laboratory Analysis

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Chronic kidney disease (CKD) is highly prevalent, with increasing numbers of patients affected by the disease world‐wide, and anemia is a common finding in patients with CKD. Anemia impacts negatively on cardiovascular disease, exercise capacity, and quality of life, resulting in significant mortality and morbidity. The aim of this study was to evaluate the levels of ischemia‐modified albumin and lactate in patients with established anemia associated with CKD and its correlations with hemoglobin levels. Hematocrit, hemoglobin, iron, ferritin, albumin, creatinine, lactate, and ischemia‐modified albumin (IMA) were measured in 17 patients with established anemia associated to CKD and 19 controls by standard methods. The results of hematocrit, hemoglobin, iron, and albumin were lower in the anemia group than in the control group. Ferritin, creatinine, and lactate levels were higher in anemia of the CKD group than the control group. IMA increase in the anemia group (0.8115±0.1304 absorbance units [ABSU]) compared to control (0.4951±0.0393 ABSU). Significant correlations between IMA and lactate, IMA and hemoglobin, IMA and creatinine, and hemoglobin and lactate were observed. IMA and lactate increase during anemia and this elevation could be associated to hypoxia due to low hemoglobin levels. However, our data suggest that lactate is more sensitive to anemia compared to IMA. J. Clin. Lab. Anal. 22:1–5, 2008. © 2008 Wiley‐Liss, Inc.

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Section: Discussionmentioning

confidence: 99%

Evaluation of ischemia‐modified albumin in anemia associated to chronic kidney disease

Cichota

Moresco

Duarte

et al. 2008

Clinical Laboratory Analysis

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“…According to Marx G and Chevion M [29], albumin, the predominant protein in circulation, undergoes site-specific modification in N-terminus, especially of the N AspAla-His-Lys sequence during overproduction of ROS resulting from conditions related to ischemia, hypoxia, acidosis, free radicals [30][31][32][33] is unable to bind transition metals like, cobalt [31]. This is the basic mechanism to detect the ischemia modified albumin.…”

Section: Discussionmentioning

confidence: 99%

Effect of Curcumin Against Oxidation of Biomolecules by Hydroxyl Radicals

Borra

Mahendra

Gurumurthy

et al. 2014

JCDR

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Background: Among various reactive oxygen species, hydroxyl radicals have the strongest chemical activity, which can damage a wide range of essential biomolecules such as lipids, proteins, and DNA.Objective: The objective of this study was to investigate the beneficial effects of curcumin on prevention of oxidative damage of biomolecules by hydroxyl radicals generated in in vitro by a Fenton like reaction. Materials and Methods:We have incubated the serum, plasma and whole blood with H 2 O 2 /Cu 2 + / Ascorbic acid system for 4 hours at 37 0 C and observed the oxidation of biomolecules like albumin, lipids, proteins and DNA.Results: Curcumin at the concentrations of 50,100 and 200 µmoles, prevented the formation of ischemia modified albumin, MDA, protein carbonyls, oxidized DNA and increased the total antioxidant levels and GSH significantly. Conclusion:These observations suggest the hydroxyl radical scavenging potentials of curcumin and protective actions to prevent the oxidation of biomolecules by hydroxyl radicals.

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“…231 It is also known that the N-terminal portion of human serum albumin is susceptible to biochemical degradation and is less stable than the albumin of other species. 232 Ischaemia-modified albumin (IMA) is a form of human serum albumin in which the N-terminal amino acids have been affected so that they are unable to bind transition metals. Measurement of IMA is with the albumin cobalt-binding (ACB ® , Ischemia Technologies, Denver, CO) test.…”

Section: Ischaemia-modified Albuminmentioning

confidence: 99%

Randomised Assessment of Treatment using Panel Assay of Cardiac markers – Contemporary Biomarker Evaluation (RATPAC CBE)

Collinson

Gaze²,

Thokala³

et al. 2013

Health Technol Assess

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Reports are published in Health Technology Assessment (HTA) if (1) they have resulted from work for the HTA programme, and (2) they are of a sufficiently high scientific quality as assessed by the reviewers and editors.Reviews in Health Technology Assessment are termed 'systematic' when the account of the search appraisal and synthesis methods (to minimise biases and random errors) would, in theory, permit the replication of the review by others. HTA programmeThe HTA programme, part of the National Institute for Health Research (NIHR), was set up in 1993. It produces high-quality research information on the effectiveness, costs and broader impact of health technologies for those who use, manage and provide care in the NHS. 'Health technologies' are broadly defined as all interventions used to promote health, prevent and treat disease, and improve rehabilitation and long-term care.The journal is indexed in NHS Evidence via its abstracts included in MEDLINE and its Technology Assessment Reports inform National Institute for Health and Care Excellence (NICE) guidance. HTA research is also an important source of evidence for National Screening Committee (NSC) policy decisions.For more information about the HTA programme please visit the website: http://www.hta.ac.uk/ This reportThe research reported in this issue of the journal was funded by the HTA programme as project number 09/22/16. The contractual start date was in August 2010. The draft report began editorial review in February 2012 and was accepted for publication in June 2012. The authors have been wholly responsible for all data collection, analysis and interpretation, and for writing up their work. The HTA editors and publisher have tried to ensure the accuracy of the authors' report and would like to thank the reviewers for their constructive comments on the draft document. However, they do not accept liability for damages or losses arising from material published in this report.This report presents independent research funded by the National Institute for Health Research (NIHR). The views and opinions expressed by authors in this publication are those of the authors and do not necessarily reflect those of the NHS, the NIHR, NETSCC, the HTA programme or the Department of Health. Published by the NIHR Journals Library, produced by Prepress Projects Ltd, Perth, Scotland (www.prepress-projects.co.uk). Objectives: To test the diagnostic accuracy for detecting an acute myocardial infarction (AMI) using highly sensitive troponin assays and a range of new cardiac biomarkers of plaque destabilisation, myocardial ischaemia and necrosis; to test the prognostic accuracy for detecting adverse cardiac events using highly sensitive troponin assays and this range of new cardiac biomarkers; and to estimate the cost-effectiveness of using highly sensitive troponin assays or this range of new cardiac biomarkers instead of an admission and 10-to 12-hour troponin measurement. NIHR Journals LibraryDesign: Substudy of the point-of-care arm of the RATPAC (Randomised Assessment ...

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Site‐specific N‐terminal Auto‐degradation of Human Serum Albumin

Cited by 103 publications

References 14 publications

Evaluation of ischemia‐modified albumin in anemia associated to chronic kidney disease

Evaluation of ischemia‐modified albumin in anemia associated to chronic kidney disease

Effect of Curcumin Against Oxidation of Biomolecules by Hydroxyl Radicals

Randomised Assessment of Treatment using Panel Assay of Cardiac markers – Contemporary Biomarker Evaluation (RATPAC CBE)

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