Site-specific distribution and competitive ability of indigenous bean-nodulating rhizobia isolated from organic fields in Minnesota

Wongphatcharachai, Manoosak; Wang, Ping; Staley, Christopher; Chun, Chan Lan; Ferguson, John A.; Moncada, Kristine M.; Sadowsky, Michael J.

doi:10.1016/j.jbiotec.2015.09.009

Cited by 3 publications

(2 citation statements)

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“…These tools include the use of antibiotic resistance markers, enzyme linked immunosorbent assay (ELISA) (Spriggs and Dakora 2009), DNA-based methods [polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) (Krasova-Wade et al 2006), enterobacterial repetitive intergenic consensus (ERIC) PCR (Ampomah et al 2008), quantitative polymerase chain reaction (qPCR) (Checcucci et al 2016) and fluorophore-enhanced, repetitive, extragenic, and palindromic-PCR (HFERP) DNA fingerprinting (Wongphatcharachai et al 2015)] and proteomic profiling by matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry (MS) (Ziegler et al 2012(Ziegler et al , 2015.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of MALDI-TOF mass spectrometry for the competitiveness analysis of selected indigenous cowpea (Vigna unguiculata L. Walp.) Bradyrhizobium strains from Kenya

Ndungu

Messmer

Ziegler³

et al. 2018

Appl Microbiol Biotechnol

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Cowpea N fixation and yield can be enhanced by selecting competitive and efficient indigenous rhizobia. Strains from contrasting agro-ecologies of Kilifi and Mbeere (Kenya) were screened. Two pot experiments were established consisting of 13 Bradyrhizobium strains; experiment 1 (11 Mbeere + CBA + BK1 from Burkina Faso), experiment 2 (12 Kilifi + CBA). Symbiotic effectiveness was assessed (shoot biomass, SPAD index and N uptake). Nodule occupancy of 13 simultaneously co-inoculated strains in each experiment was analyzed by matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry (MS) to assess competitiveness. Strains varied in effectiveness and competitiveness. The four most efficient strains were further evaluated in a field trial in Mbeere during the 2014 short rains. Strains from bacteroids of cowpea nodules from pot and field experiments were accurately identified as Bradyrhizobium by MALDI-TOF based on the SARAMIS™ database. In the field, abundant indigenous populations 7.10 × 10 rhizobia g soil, outcompeted introduced strains. As revealed by MALDI-TOF, indigenous strains clustered into six distinct groups (I, II, III, IV, V and VI), group III were most abundant occupying 80% of nodules analyzed. MALDI-TOF was rapid, affordable and reliable to identify Bradyrhizobium strains directly from nodule suspensions in competition pot assays and in the field with abundant indigenous strains thus, its suitability for future competition assays. Evaluating strain competitiveness and then symbiotic efficacy is proposed in bioprospecting for potential cowpea inoculant strains.

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Section: Introductionmentioning

confidence: 99%

“…In the above-mentioned competition studies (Checcucci et al 2016;Krasova-Wade et al 2006;Spriggs and Dakora 2009;Wongphatcharachai et al 2015), assays of two coinoculated strains were assessed. Using ERIC-PCR, Ampomah et al (2008) carried out a competition assay in which up to 5 strains were assessed simultaneously.…”

Section: Introductionmentioning

confidence: 99%