Site‐Specific Control of Distances between Gold Nanoparticles Using Phosphorothioate Anchors on DNA and a Short Bifunctional Molecular Fastener

Abstract

Going for double gold: Precise control of the positions of and distances between gold nanoparticles (AuNP; gold spheres in picture) is achieved by linking the AuNPs to phosphorothioate‐modified DNA (green and red helices) with a bifunctional fastener. The distance between AuNPs is controlled simply by changing the position of the modifications on the DNA template.

Quantum dots–DNA bioconjugates: synthesis to applications

“…3000Â) for Cd 2þ than oxygen and hence forms stronger complexes with the inorganic shells, including QDs. First demonstrated by Kumar et al on colloidal gold, this affinity has been utilized to conjugate phosphorothioate-modified DNA to gold nanoparticles (GNPs) [53,54] and later to QDs [55][56][57] either post or during synthesis. This method is advantageous to link DNA on NPs, but suffers from the limitation that the 'attached' DNA is conformationally distorted and often loses its ability to hybridize complementary DNA [56].…”

Cleavable Molecular Beacon for Hg2+ Detection Based on Phosphorothioate RNA Modifications

“…14,[18][19][20][21][22] In addition, 4 because of its affinity for thiophilic metals, PS-modified DNA can functionalize and assemble both metal and semiconductor nanoparticles. [23][24][25][26][27] We recently selected a lanthanide-dependent DNAzyme, named Ce13d. 28 Gel-based assays.…”

Sensing Parts-per-Trillion Cd2+, Hg2+, and Pb2+ Collectively and Individually Using Phosphorothioate DNAzymes

“…The PS modification is often used in the antisense technology to increase DNA stability against nuclease degradation. 28 It is also useful for studying the mechanism of (deoxy)ribozyme catalysis, [29][30][31][32] assembling nanoparticles, 33 and forming DNA structures. 34 However, PS-modified RNA-cleaving DNAzymes have not yet been studied.…”