Site-Specific Association of c-SRC with Epidermal Growth Factor Receptor in A431 Cells

Adaptor protein Shc undergoes translocation and mediates up‐regulation of the tyrosine kinase c‐Src in EGF‐stimulated A431 cells

“…As we described in the Introduction section, we have previously demonstrated that, in A431 cells, EGF induces an association of EGFR with c-Src and the activation of c-Src (Sato et al 1995a). So, it is attractive to surmise that EGFR, GFP-Shc and c-Src assemble with each other in EGF-stimulated A431 cells.…”

Modulation of hepatic acute phase gene expression by epidermal growth factor and src protein tyrosine kinases in murine and human hepatic cells

“…In as much as the intrinsic PTK activity of EGFR is critical for the overall signal initiation process, receptor-recruited Src kinases are implicated to participate in signal propagation. 26,28,65,66 Because association of Src kinases has similarly been detected with IL-6R cytokine receptors and the ability of Src kinases to engage STATs has been noted before, we wanted to determine the extent to which JAK and Src kinases act as mediators of EGF and IL-6-regulated APPs. First, we determined the kinase specificity of STAT3 activation by gp130 and EGFR.…”

“…Signaling to STAT3 and the reporter gene by EGFR, however, was mostly prevented by Csk (lanes 12 and 13), suggesting that a Csk-sensitive pathway communicates between EGFR, STAT3, and IL-6RE. To assess whether Src kinases were found in association with EGFR and therefore could account for signal communication as reported for EGFR in other cell systems, 28,65,66 we determined by coimmunoprecipitation the complex of c-Src with endogenous EGFR in clone 86-6 HepG2 cells (Fig. 4B) and in mouse hepatocytes (Fig.…”

Inhibition of pp60c-Src reduces Bcl-XL expression and reverses the transformed phenotype of cells overexpressing EGF and HER-2 receptors

“…pp60 c-Src (c-Src) has been shown to associate directly with the platelet derived growth factor receptor (PDGFR) (Broome and Hunter, 1996) and CSF-1 receptor (Courtneidge et al, 1993). Src was also reported to interact with EGFR (Sato et al, 1995;Stover et al, 1995), and to phosphorylate the HER-2/ neu and IGF-1 receptors (Stover et al, 1995;Peterson et al, 1996). In these cases, c-Src phosphorylates unique phosphorylation sites on these receptors that serve as docking sites for downstream signal transducers like p85, the regulatory subunit of PI 3'-kinase and Shc (Stover et al, 1995).…”