Site-selective heat boosting electrochemiluminescence for single cell imaging

Abstract

In operando visualization of local electrochemical reactions provides mechanical insights into the dynamic transport of interfacial charge and reactant/product. Electrochemiluminescence is a crossover technique that quantitatively determines faraday current and...

Electrochemiluminescence Microscopy

“…Gou et al addressed this issue by accelerating the reaction rate and enhancing thermal convection of the ECL process through a photothermal effect. [76] This advancement led to a notable 20-fold increase in signal contrast for ECL cell imaging, offering an effective solution to the weak light emission issue. In the abovedescribed works, ECL labels were required for successful visualization.…”

Infrared photoinduced electrochemiluminescence microscopy of single cells

“…11,12 Since ECL provides an optical readout, in the last decade, it has evolved into a powerful technique for microscopy applications, allowing for the study of micro/nanoparticles and biological entities such as cells or organelles. 13–23 Two main imaging approaches have been developed: (i) positive ECL (ECL+) with a light-emitting object, which directly generates ECL or is labeled with an ECL tag, on a dark background; and (ii) shadow or negative label-free ECL (ECL−), which is based on a non-ECL emissive object hindering the ECL reagents diffusion and thus shadowing the ECL background. 18,24 Both provide complementary information about the imaged object.…”

Shadow electrochemiluminescence imaging of giant liposomes opening at polarized electrodes

“…40–44 In addition, it was demonstrated that ECL can be performed under microscopy, 45–50 allowing the imaging of single micro- and nano-objects, 51–58 particularly those of biological interest. 59–70…”