Site-directed placement of three-dimensional DNA origami

Abstract

Assembling hybrid substrates with nanometer-scale precision and molecular addressability enables advances in such distant elds as material research and biosensing. As such, the combination of lithographic methods with 2D DNA origami self-assembly has led, among others, to the development of photonic crystal cavity arrays and the exploration of sensing nanoarrays where molecular devices are patterned on the sub-micron scale. Here we extend this concept to the third dimension through mounting 3D DNA origami onto nano-patterned substrates followed by silici cation to provide mechanical and chemical stability. Our versatile and scalable method relying on self-assembly at ambient temperatures offers the potential to 3D-position any inorganic and organic components that are compatible with DNA architectures. This way, complex and 3D-patterend surfaces designed on the molecular level while reaching macroscopic dimensions could supersede energy-intensive manufacturing steps in substrate processing.

Self-assembled methodologies for the construction of DNA nanostructures and biological applications

“…1d). 32 DNA origami building blocks can also be used to form lattices and crystals based on the interactions including sticky-ended hybridization, blunt-ended interactions/shape complementarity, and substrate-assisted self-assembly. 1,16,33,34 Liedl's group proposed a 3D rhombohedral crystalline assembled with a DNA-origami-based “tensegrity triangle” structure (Fig.…”

DNA-Based Gold Nanoparticle Assemblies: From Structure Constructions to Sensing Applications

“…Bottom-up assembly based on DNA origami can also be combined with top-down lithography [70]. Martynenko et al demonstrated a method for the precisely targeted placement of various shapes of DNA origami on the micrometer to millimeter scale [71]. They utilized two approaches of connector-mediated binding (hollow tubes) and direct binding (the tetrapod) through self-aligned binding to realize the upright positioning of various DNA origami shapes.…”

“…(c) AuNPs assembled on the DNA origami structure and placed on the x-y plane to form a periodic array. Reprinted with permission from ref [71]…”

Laser-Based Reshaping of Self-Organized Metasurfaces of Supported Ag Nanoparticles for Encoding Tunable Linear Dichroism Patterns

“…These applications are possible due to the excitation of localized surface plasmon resonance in metallic nanoparticles (NPs) (collective oscillations of conduction electrons) when interacting with incident light. The spectral position of the plasmon resonance depends on the composition and size of the nanoparticle, the dielectric environment, and to a greater extent on the shape of the NP. − In relatively dense arrays of such NPs, the interparticle plasmonic coupling will cause changes in the plasmon resonance characteristics and the extinction spectrum. − Moreover, for individual anisotropic NPs or as in array, the absorption and scattering will strongly depend on the polarization of the incident electromagnetic wave. − More complex structures, such as chiral nanostructures, are more challenging to produce. , These can be either individual anisotropic nanoparticles obtained by chemical synthesis , or complexes of isotropic NPs helically arranged, for example, on DNA. , The latter method shows promising possibilities for obtaining complex chiral structures using DNA origami to obtain various plasmonic “enantiomers” from nanorods or nanospheres. − …”