Site-Directed Mutagenesis in Barley by Expression of TALE Nuclease in Embryogenic Pollen

Gurushidze, Maia; Hiekel, Stefan; Otto, Ingrid; Hensel, Goetz; Kumlehn, Jochen

doi:10.1007/978-3-319-45021-6_7

Cited by 7 publications

(4 citation statements)

References 28 publications

(23 reference statements)

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“…Double-haploid plants containing two identical sets of chromosomes can then be generated from the haploid lines in a second step. Haploid induction can be used to e.g., produce homozygous plant lines from genome edited plants (Gurushidze et al, 2017 ). However, CENH3-mediated haploid induction could be applied as a general tool to speed up all breeding activities by substituting time-consuming back-crossing steps with the faster HI approach.…”

Section: Risk Assessment Considerationsmentioning

confidence: 99%

An EU Perspective on Biosafety Considerations for Plants Developed by Genome Editing and Other New Genetic Modification Techniques (nGMs)

Eckerstorfer

Dolezel

Heissenberger

et al. 2019

Front. Bioeng. Biotechnol.

101

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The question whether new genetic modification techniques (nGM) in plant development might result in non-negligible negative effects for the environment and/or health is significant for the discussion concerning their regulation. However, current knowledge to address this issue is limited for most nGMs, particularly for recently developed nGMs, like genome editing, and their newly emerging variations, e.g., base editing. This leads to uncertainties regarding the risk/safety-status of plants which are developed with a broad range of different nGMs, especially genome editing, and other nGMs such as cisgenesis, transgrafting, haploid induction or reverse breeding. A literature survey was conducted to identify plants developed by nGMs which are relevant for future agricultural use. Such nGM plants were analyzed for hazards associated either (i) with their developed traits and their use or (ii) with unintended changes resulting from the nGMs or other methods applied during breeding. Several traits are likely to become particularly relevant in the future for nGM plants, namely herbicide resistance (HR), resistance to different plant pathogens as well as modified composition, morphology, fitness (e.g., increased resistance to cold/frost, drought, or salinity) or modified reproductive characteristics. Some traits such as resistance to certain herbicides are already known from existing GM crops and their previous assessments identified issues of concern and/or risks, such as the development of herbicide resistant weeds. Other traits in nGM plants are novel; meaning they are not present in agricultural plants currently cultivated with a history of safe use, and their underlying physiological mechanisms are not yet sufficiently elucidated. Characteristics of some genome editing applications, e.g., the small extent of genomic sequence change and their higher targeting efficiency, i.e., precision, cannot be considered an indication of safety per se, especially in relation to novel traits created by such modifications. All nGMs considered here can result in unintended changes of different types and frequencies. However, the rapid development of nGM plants can compromise the detection and elimination of unintended effects. Thus, a case-specific premarket risk assessment should be conducted for nGM plants, including an appropriate molecular characterization to identify unintended changes and/or confirm the absence of unwanted transgenic sequences.

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Section: Risk Assessment Considerationsmentioning

confidence: 99%

An EU Perspective on Biosafety Considerations for Plants Developed by Genome Editing and Other New Genetic Modification Techniques (nGMs)

Eckerstorfer

Dolezel

Heissenberger

et al. 2019

Front. Bioeng. Biotechnol.

101

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“…These aforementioned endonucleases can be customized to target a specific DNA sequence motif within live cells, and subsequently, the cellular DNA repair machinery processes the cleaved DNA. The mechanisms of cellular DNA repair include non-homologous end-joining (NHEJ) and homology-directed repair (HDR) [102] CRISPR CRISPR genome-editing technology offers exciting opportunities for engineering desirable traits in plants with precise genome engineering and without the need for transgene integration. The CRISPR/Cas platform is based on RNA-guided Cas endonucleases, which are derived from the adaptive immune system of microbes.…”

Section: Genome Editing In Oatsmentioning

confidence: 99%

From Petri Dish to Field: Plant Tissue Culture and Genetic Engineering of Oats for Improved Agricultural Outcomes

Pathi,

Sprink

2023

Plants

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Oats (Avena sativa) hold immense economic and nutritional value as a versatile crop. They have long been recognized as an exceptional choice for human consumption and animal feed. Oats’ unique components, including proteins, starches, and β-glucans, have led to its widespread use in various food products such as bread, noodles, flakes, and milk. The popularity of oat milk as a vegan alternative to dairy milk has soared due to the increasing number of vegetarians/vegans and growing environmental awareness. Oat milk offers a sustainable option with reduced greenhouse gas emissions during its production, rendering it an appropriate choice for individuals who are lactose-intolerant or have dairy allergies. To ensure improved adaptability and enhanced nutrition, the development of new oat varieties is crucial, considering factors like cultivation, climate, and growing conditions. Plant cell culture plays a crucial role in both traditional and contemporary breeding methods. In classical breeding, plant cell culture facilitates the rapid production of double haploid plants, which can be employed to accelerate the breeding process. In modern breeding methods, it enables genetic manipulation and precise genome editing at the cellular level. This review delves into the importance of oats and their diverse applications, highlighting the advantages of plant cell culture in both classical and modern breeding methods. Specifically, it provides an overview of plant tissue culture, encompassing genetic transformation, haploid technology, protoplast technology, and genome editing.

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“…Plants that have been irradiated with gamma-rays may germinate faster as a result. Furthermore, gamma-ray-induced mutations are known to be the most effective in a wide range of mutations because gamma-rays penetrate deeply into target tissues, are less destructive, and can produce a broader spectrum of mutations raging from SNPs and small indels to deletions greater than one million base pairs ( Gurushidze et al, 2017 ; Ramchander et al, 2022 ).…”

Section: Impact and Mode Of Action Of Important Physical Mutagens On ...mentioning

confidence: 99%

A systematic review on physical mutagens in rice breeding in Southeast Asia

Baadu,

Chong,

Gansau

et al. 2023

PeerJ

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In the 1920s, Lewis Stadler initiated the introduction of permanent improvements to the genetic makeup of irradiated plants. Since then, studies related to breeding mutations have grown, as efforts have been made to expand and improve crop productivity and quality. Stadler’s discovery began with x-rays on corn and barley and later extended to the use of gamma-rays, thermal, and fast neutrons in crops. Radiation has since been shown to be an effective and unique method for increasing the genetic variability of species, including rice. Numerous systematic reviews have been conducted on the impact of physical mutagens on the production and grain quality of rice in Southeast Asia. However, the existing literature still lacks information on the type of radiation used, the rice planting materials used, the dosage of physical mutagens, and the differences in mutated characteristics. Therefore, this article aims to review existing literature on the use of physical mutagens in rice crops in Southeast Asian countries. Guided by the PRISMA Statement review method, 28 primary studies were identified through a systematic review of the Scopus, Science Direct, Emerald Insight, Multidisciplinary Digital Publishing, and MDPI journal databases published between 2016 and 2020. The results show that 96% of the articles used seeds as planting materials, and 80% of the articles focused on gamma-rays as a source of physical mutagens. The optimal dosage of gamma-rays applied was around 100 to 250 Gy to improve plant development, abiotic stress, biochemical properties, and nutritional and industrial quality of rice.

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Site-Directed Mutagenesis in Barley by Expression of TALE Nuclease in Embryogenic Pollen

Cited by 7 publications

References 28 publications

An EU Perspective on Biosafety Considerations for Plants Developed by Genome Editing and Other New Genetic Modification Techniques (nGMs)

An EU Perspective on Biosafety Considerations for Plants Developed by Genome Editing and Other New Genetic Modification Techniques (nGMs)

From Petri Dish to Field: Plant Tissue Culture and Genetic Engineering of Oats for Improved Agricultural Outcomes

A systematic review on physical mutagens in rice breeding in Southeast Asia

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