Site-directed introduction of disulfide groups on antibodies for highly sensitive immunosensors

Sánchez, Josep Ll. Acero; Fragoso, Alex; Joda, Hamdi; Suárez, Guillaume; McNeil, Calum J.; O’Sullivan, Ciara K.

doi:10.1007/s00216-016-9630-9

Cited by 15 publications

(4 citation statements)

References 38 publications

(2 reference statements)

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“…Research efforts with mixed OEG films have sought to create surfaces that have the potential to serve as biosensors. ,, For example, Jeong and co-workers grafted poly(ethylene glycol methacrylate) brushes from the surface of a mixed SAM and determined that the concentration of the initiator molecule in the SAM dictated the lateral packing density and the overall amount of bound streptavidin . Additionally, Clare and co-workers expanded research on OEG-terminated films by covalently attaching OEG monolayers to silicon and diamond surfaces as a potential electronic biosensor .…”

Section: Pursuit Of Uniquely Blended Interfacesmentioning

confidence: 99%

Homogeneously Mixed Monolayers: Emergence of Compositionally Conflicted Interfaces

et al. 2017

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The ability to manipulate interfaces at the nanoscale via a variety of thin-film technologies offers a plethora of avenues for advancing surface applications. These include surfaces with remarkable antibiofouling properties as well as those with tunable physical and electronic properties. Molecular self-assembly is one notably attractive method used to decorate and modify surfaces. Of particular interest to surface scientists has been the thiolate-gold system, which serves as a reliable method for generating model thin-film monolayers that transform the interfacial properties of gold surfaces. Despite widespread interest, efforts to tune the interfacial properties using mixed adsorbate systems have frequently led to phase-separated domains of molecules on the surface with random sizes and shapes depending on the structure and chemical composition of the adsorbates. This feature article highlights newly emerging methods for generating mixed thin-film interfaces, not only to enhance the aforementioned properties of organic thin films, but also to give rise to interfacial compositions never before observed in nature. An example would be the development of monolayers formed from bidentate adsorbates and other unique headgroup architectures that provide the surface bonding stability necessary to allow the assembly of interfaces that expose mixtures of chains that are fundamentally different in character (i.e., either phase-incompatible or structurally dissimilar), producing compositionally "conflicted" interfaces. By also exploring the prior efforts to produce such homogeneously blended interfaces, this feature article seeks to convey the relationships between the methods of film formation and the overall properties of the resulting interfaces.

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Section: Pursuit Of Uniquely Blended Interfacesmentioning

confidence: 99%

Homogeneously Mixed Monolayers: Emergence of Compositionally Conflicted Interfaces

et al. 2017

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“…SAMs composed of 4-mercaptophenylboronic acid (4-MBA) formed directly on gold [ 71 ] and 3-aminophenylboronic acid, immobilized on modified SPR transducers using graphene oxide carboxyl groups, have been described as compounds directly responsible for the capture of antibodies [ 74 ]. Additionally, amine-terminated ligands can be employed for carbohydrate-mediated immobilization through the previous oxidation of polysaccharide residues to aldehydes, followed by Schiff base formation [ 75 ].…”

Section: Functional Surfaces and Non-oriented Ab Immobilization Strategies For Spr Immunosensingmentioning

confidence: 99%

Recent Advancements in Receptor Layer Engineering for Applications in SPR-Based Immunodiagnostics

Drozd

Karoń

Malinowska

2021

Sensors

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The rapid progress in the development of surface plasmon resonance-based immunosensing platforms offers wide application possibilities in medical diagnostics as a label-free alternative to enzyme immunoassays. The early diagnosis of diseases or metabolic changes through the detection of biomarkers in body fluids requires methods characterized by a very good sensitivity and selectivity. In the case of the SPR technique, as well as other surface-sensitive detection strategies, the quality of the transducer-immunoreceptor interphase is crucial for maintaining the analytical reliability of an assay. In this work, an overview of general approaches to the design of functional SPR-immunoassays is presented. It covers both immunosensors, the design of which utilizes well-known and often commercially available substrates, as well as the latest solutions developed in-house. Various approaches employing chemical and passive binding, affinity-based antibody immobilization, and the introduction of nanomaterial-based surfaces are discussed. The essence of their influence on the improvement of the main analytical parameters of a given immunosensor is explained. Particular attention is paid to solutions compatible with the latest trends in the development of label-free immunosensors, such as platforms dedicated to real-time monitoring in a quasi-continuous mode, the use of in situ-generated receptor layers (elimination of the regeneration step), and biosensors using recombinant and labelled protein receptors.

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“…Several immobilization methods such as self-assembled monolayers (SAM) [19], electropolymerization [20], site-directed techniques [21], silanization [22] and direct covalent binding [23] have been utilized in the fabrication of immunosensors to handle these problems [24]. Immunosensors offer a number of advantages over traditional analytical techniques, including portability, specificity, cheapness and real-time monitoring as well as having good versatility, robustness, selectivity and sensitivity depending on the transducer [25].…”

Section: Introductionmentioning

confidence: 99%

Development of a functional impedimetric immunosensor for accurate detection of thy- roid-stimulating hormone

Asav¹

2021

Turk J Chem

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Thyroid-stimulating hormone (TSH) that regulates the synthesis of thyroid gland hormones controlling all metabolism is one of the pituitary hormones. Determination of TSH is crucial for monitoring thyroid gland-related disorders and some metabolic diseases.In this study, a non-labeled immunosensor based on covalent immobilization of anti-TSH antibody by using the formation of self-assembled monolayers (SAM) of 4mercaptophenylacetic acid (4-MPA) and functionalization of carboxyl ends with 1ethyl-3-(3-dimetilaminopropil) carbodiimide (EDC)/N-Hydroxysuccinimide (NHS) was fabricated for detection of TSH. Immobilization steps including the concentration of 4-2 MPA, the concentration of anti-TSH antibody and duration of anti-TSH antibody incubation were optimized by utilizing electrochemical impedance spectroscopy. Under optimal conditions, a sensitive, rapid and accurate determination of TSH at a concentration range between 0.7 -3.5 mIU/L was accomplished with a notable linearity and LOD value as 0.034 mIU/L as well as reproducibility and repeatability. Moreover, for comparison, linear range experiments were also carried out by using other electrochemical methods including Linear Sweep Voltammetry (LSV), Cyclic Voltammetry (CV) and capacitance spectroscopy (C). Finally, the constructed immunosensor was used for analyzing TSH levels spiked in the artificial serum samples.

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Site-directed introduction of disulfide groups on antibodies for highly sensitive immunosensors

Cited by 15 publications

References 38 publications

Homogeneously Mixed Monolayers: Emergence of Compositionally Conflicted Interfaces

Homogeneously Mixed Monolayers: Emergence of Compositionally Conflicted Interfaces

Recent Advancements in Receptor Layer Engineering for Applications in SPR-Based Immunodiagnostics

Development of a functional impedimetric immunosensor for accurate detection of thy- roid-stimulating hormone

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